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Schumannella soli sp. nov., a novel actinomycete isolated from mangrove soil by in situ cultivation

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Abstract

A novel actinobacterial strain, designated 10F1D-1T, was isolated from soil sample collected from Futian mangrove nature reserve, China using of the in situ cultivation technique. Preliminary analysis based on the 16S rRNA gene sequence revealed that strain 10F1D-1T was the member of genus Schumannella with sharing highest sequence similarity (99.7%) to Schumannella luteola DSM 23141T. Phylogenetic analyses based on 16S rRNA gene sequences and core proteome consistently exhibited that strain 10F1D-1T formed a monophyletic clade with Schumannella luteola DSM 23141T. Comparative genomic analyses clearly separated strain 10F1D-1T from the only species of the genus Schumannella based on average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values below the thresholds for species delineation. The genome of strain 10F1D-1T contains the biosynthetic gene clusters for osmoprotectants to adapt to the salt environment of mangrove. Strain 10F1D-1T also contains the biosynthetic gene clusters for bioactive compounds as secondary metabolites. On the basis of the polyphasic analysis, strain 10F1D-1T is considered to represent a novel species of the genus Schumannella, for which the name Schumannella soli sp. nov. (type strain 10F1D-1T = CGMCC1.16699T = JCM 33146T) is proposed.

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Data availability

All data generated and analyzed in present study are included in this published article, supplementary files and GenBank/EMBL/DDBJ.

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Funding

This research was partly supported by the Drug Innovation Major Project of China (Grant No. 2018ZX09711001-007-001), CAMS Innovation Fund for Medical Sciences (Grant No. CAMS 2017-I2M-B&R-08) and PUMC Youth Fund (Grant No. 2017350022).

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Authors and Affiliations

  1. Laboratory of Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Center for Children’s Health, Beijing, 100045, China

    Feina Li & Adong Shen

  2. Beijing Key Laboratory of Antimicrobial Agents, Department of Microbial Chemistry, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China

    Feina Li, Qinpei Lu, Shaowei Liu, Qin Yang & Chenghang Sun

  3. Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, 510005, China

    Shuilin Liao

  4. Life Sciences Institute, Zunyi Medical University, Zunyi, 563006, China

    Li Tuo

Authors
  1. Feina Li
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  2. Qinpei Lu
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  3. Shuilin Liao
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  4. Li Tuo
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  5. Shaowei Liu
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  6. Qin Yang
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  7. Adong Shen
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  8. Chenghang Sun
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Contributions

FL designed the experiments and performed the identification. CS, FL, QL and SL carried out the sampling. SL, LT and QY supervised the phylogenetic analysis. QL and SL supervised the analyses of menaquinone and fatty acid. FL, CS and AS analyzed the results, wrote and approved the manuscript. All authors have read and agreed to the published version of the manuscript.

Corresponding authors

Correspondence to Adong Shen or Chenghang Sun.

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Li, F., Lu, Q., Liao, S. et al. Schumannella soli sp. nov., a novel actinomycete isolated from mangrove soil by in situ cultivation. Antonie van Leeuwenhoek 114, 1657–1667 (2021). https://doi.org/10.1007/s10482-021-01631-6

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  • DOI: https://doi.org/10.1007/s10482-021-01631-6

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