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Papiliotrema phichitensis f.a., sp. nov., a novel yeast species isolated from sugarcane leaf in Thailand

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Abstract

Strain DMKU-SP105T representing a novel yeast species was isolated from the external surface of a sugarcane leaf (Saccharum officinarum L.) collected from a sugarcane plantation field in Phichit province, Thailand. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, the strain DMKU-SP105T differed by 7–16 substitutions in the D1/D2 region of LSU rRNA gene and 6–22 substitutions in the ITS region from a group of related species, Papiliotrema aspenensis, Papiliotrema odontotermitis, Papiliotrema rajasthanensis and Papiliotrema laurentii. A phylogenetic analysis based on the concatenated sequences of ITS region and the D1/D2 region of the LSU rRNA gene indicated that strain DMKU-SP105T belongs to the laurentii clade of Papiliotrema in the Tremellales and is distinct from other related species in the clade. It therefore represents a novel species of the genus Papiliotrema although the formation of basidiospores was not observed. The name Papiliotrema phichitensis f.a., sp. nov. is proposed. The type is DMKU-SP105T (= CBS 13390T = BCC 61187T = NBRC 109699T).

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Funding

This work was supported by the Thailand Research Fund through the TRF Research-Team Promotion Grant (RTA5480009 and RTA6080004).

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

    Pannida Khunnamwong, Janjira Surussawadee, Nantana Srisuk, Chanita Boonmak & Savitree Limtong

  2. Academy of Science, The Royal Society of Thailand, Bangkok, 10300, Thailand

    Savitree Limtong

Authors
  1. Pannida Khunnamwong
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  2. Janjira Surussawadee
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  3. Nantana Srisuk
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  4. Chanita Boonmak
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  5. Savitree Limtong
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Contributions

PK: performed research and wrote the paper; JS: performed research; NS: analyzed data; CB: analyzed data; SL: designed study and wrote the paper.

Corresponding author

Correspondence to Savitree Limtong.

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The authors declare that there are no conflicts of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

The GenBank/EMBL/DDBJ accession numbers for the combined sequences of the ITS region and the D1/D2 region of the LSU rRNA gene of strain DMKU-SP105 are AB915388 and AB826437, respectively. The MycoBank number for Papiliotrema phichitensis f.a., sp. nov. is MB 825565.

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Khunnamwong, P., Surussawadee, J., Srisuk, N. et al. Papiliotrema phichitensis f.a., sp. nov., a novel yeast species isolated from sugarcane leaf in Thailand. Antonie van Leeuwenhoek 111, 2455–2461 (2018). https://doi.org/10.1007/s10482-018-1134-5

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  • DOI: https://doi.org/10.1007/s10482-018-1134-5

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