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Microbial diversity in acidic thermal pools in the Uzon Caldera, Kamchatka

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Abstract

Microbial communities of four acidic thermal pools in the Uzon Caldera, Kamchatka, Russia, were studied using amplification and pyrosequencing of 16S rRNA gene fragments. The sites differed in temperature and pH: 1805 (60 °C, pH 3.7), 1810 (90 °C, pH 4.1), 1818 (80 °C, pH 3.5), and 1807 (86 °C, pH 5.6). Archaea of the order Sulfolobales were present among the dominant groups in all four pools. Acidilobales dominated in pool 1818 but were a minor fraction at the higher temperature in pool 1810. Uncultivated Archaea of the Hot Thaumarchaeota-related clade were present in significant quantities in pools 1805 and 1807, but they were not abundant in pools 1810 and 1818, where high temperatures were combined with low pH. Nanoarchaeota were present in all pools, but were more abundant in pools 1810 and 1818. A similar abundance pattern was observed for Halobacteriales. Thermophilic Bacteria were less diverse and were mostly represented by aerobic hydrogen- and sulfur-oxidizers of the phylum Aquificae and sulfur-oxidising Proteobacteria of the genus Acidithiobacillus. Thus we showed that extremely acidic hot pools contain diverse microbial communities comprising different metabolic groups of prokaryotes, including putative lithoautotrophs using energy sources of volcanic origin, and various facultative and obligate heterotrophs.

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Acknowledgements

This work was supported by the Russian Science Foundation [Grant Number 14-14-01016]. The work of AVM and VMG was supported by the Russian Foundation for Basic Research [Grant Number 11-04-00671] and the Program “Molecular and Cellular Biology” of the Russian Academy of Sciences [project of AVM]. We are grateful to the Editor, Dr. Brian P. Hedlund, and three anonymous reviewers for their appropriate and constructive suggestions and for their proposed corrections to improve the manuscript. We thank Dr. Elizaveta Bonch-Osmolovskaya, her colleagues from the Winorgadsky Institute of Microbiology RAS (Moscow, Russia), and the staff of the Kronotsky Nature Reserve for their assistance in field studies in the Uzon Caldera. This work was performed using the scientific equipment of the Core Research Facility “Bioengineering”.

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  1. Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Prosp., 33-2, Moscow, Russia, 119071

    Andrey V. Mardanov, Vadim M. Gumerov, Alexey V. Beletsky & Nikolai V. Ravin

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  1. Andrey V. Mardanov
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  2. Vadim M. Gumerov
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  3. Alexey V. Beletsky
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Correspondence to Nikolai V. Ravin.

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Mardanov, A.V., Gumerov, V.M., Beletsky, A.V. et al. Microbial diversity in acidic thermal pools in the Uzon Caldera, Kamchatka. Antonie van Leeuwenhoek 111, 35–43 (2018). https://doi.org/10.1007/s10482-017-0924-5

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