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Enterobacter tabaci sp. nov., a novel member of the genus Enterobacter isolated from a tobacco stem

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Abstract

A Gram-stain negative, motile, rod-shaped bacterium, designated strain YIM Hb-3T, was isolated from the stem of a tobacco plant. The strain was observed to form convex, circular and yellow-colored colonies. The predominant respiratory quinone was identified as Q-8. The major fatty acids (>5 %) detected were C16:1ω7c and/or C16:1ω6c (summed feature 3), C16:0, C17:0cyclo, C18:1ω7c and/or C18:1ω6c (summed feature 8), C14:03-OH and/or iso-C16:1I (summed feature 2), C14:0 and C12:0. The genomic DNA G+C content was determined to be 54.8 mol%. Phylogenetic trees based on 16S rRNA gene sequences and multilocus sequence analysis showed that strain YIM Hb-3T had the closest phylogenetic relationship with Enterobacter mori LMG 25706T. DNA–DNA relatedness value between strain YIM Hb-3T and E. mori LMG 25706T was 46.9 ± 3.8 %. On the basis of phenotypic and chemotaxonomic data, phylogenetic analysis, and DNA–DNA relatedness value, strain YIM Hb-3T is considered to represent a novel species of the genus Enterobacter, for which the name Enterobacter tabaci sp. nov. is proposed. The type strain is YIM Hb-3T (=KACC 17832T =KCTC 42694T).

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Acknowledgments

The authors are grateful to Miss Sang Mi Lee (KCTC) and ClaudineVereecke (LMG) for their kind providing the reference type strains. This research was supported by National Natural Science Foundation of China (No. 31160432), China National Tobacco Corporation (110201402040) and HKZB201508012-51, and by Foundation of Yunnan Province (Nos. 2012BA015 and 2014-01). W.-J. Li was also supported by Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme (2014).

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    Authors and Affiliations

    1. China Tobacco Yunnan Industrial Co. Ltd, Kunming, 650231, People’s Republic of China

      Yan-Qing Duan, Xing-Kui Zhou & Li-Zhi Dang

    2. Life Science College, Southwest Forestry University, Kunming, 650224, People’s Republic of China

      Xing-Kui Zhou

    3. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

      Li Di-Yan, Yong-Guang Zhang, Li-Hong Qiu, Salam Nimaichand & Wen-Jun Li

    4. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People’s Republic of China

      Yong-Guang Zhang & Wen-Jun Li

    5. Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China

      Li Di-Yan

    6. Kunming Xianghao Technology Co. Ltd., Kunming, 650204, People’s Republic of China

      Qing-Qing Li

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    1. Yan-Qing Duan
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    Yan-Qing Duan, Xing-Kui Zhou and Li Di-Yan contributed equally to this work.

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    Duan, YQ., Zhou, XK., Di-Yan, L. et al. Enterobacter tabaci sp. nov., a novel member of the genus Enterobacter isolated from a tobacco stem. Antonie van Leeuwenhoek 108, 1161–1169 (2015). https://doi.org/10.1007/s10482-015-0569-1

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