Skip to main content
SpringerLink
Log in

Sporosarcina siberiensis sp. nov., isolated from the East Siberian Sea

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A Gram-stain positive, non-motile, rod-shaped bacterium, designated strain 1111S-42T, was isolated from the East Siberian Sea. The organism was found to grow at 4–30 °C, pH 7.0–8.5 and in 0–8 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 7.5 and in 1 % NaCl. Based on 16S rRNA gene sequence similarity studies, strain 1111S-42T was found to belong to the genus Sporosarcina and to be most closely related to Sporosarcina contaminans CCUG53915T (97.3 %) and Sporosarcina soli I80T (97.2 %). The main polar lipids were found to include diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant menaquinone was identified as MK-7. The major cellular fatty acids were identified as anteiso-C15:0 (34.4 %), iso-C15:0 (29.8 %) and anteiso-C17:0 (22.4 %). The DNA G+C content of strain 1111S-42T was determined to be 39 mol %. The values of DNA–DNA relatedness between the strain 1111S-42T and related type strains of the genus Sporosarcina were less than 30 %. Based on the phylogenetic analysis, along with extensive physiological and chemotaxonomic testing, we conclude that the bacterium represents a novel species of the genus Sporosarcina, for which the name Sporosarcina siberiensis sp. nov. is proposed. The type strain is strain 1111S-42T (=CGMCC 1.12516T = LMG 27494T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • An SY, Haga T, Kasai H, Goto K, Yokota A (2007) Sporosarcina saromensis sp. nov., an aerobic endospore-forming bacterium. Int J Syst Evol Microbiol 57(8):1868–1871

    Article  PubMed  Google Scholar 

  • Cerny G (1978) Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5(2):113–122

    Article  CAS  Google Scholar 

  • Chester FD (1898) Report of the mycologist: bacteriological work. Del Agric Exp Stn Bull 10:47–137

    Google Scholar 

  • Collins M, Jones D (1980) Lipids in the Classification and Identification of Coryneform Bacteria Containing Peptidoglycans Based on 2, 4-diaminobutyric Acid. J Appl Microbiol 48(3):459–470

    CAS  Google Scholar 

  • Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100(2):221–230

    Article  CAS  PubMed  Google Scholar 

  • Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  • Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. The J Gen Appl Microbiol 29(4):319–322

    Article  CAS  Google Scholar 

  • Kampfer P, Falsen E, Lodders N, Schumann P (2010) Sporosarcina contaminans sp. nov. and Sporosarcina thermotolerans sp. nov., two endospore-forming species. Int J Syst Evol Microbiol 60(6):1353–1357

    Article  CAS  PubMed  Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62(Pt 3):716–721

    Article  CAS  PubMed  Google Scholar 

  • Kluyver AJ, van Niel CB (1936) Prospects for a natural classification of bacteria. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt II 94:369–403

    Google Scholar 

  • Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5(12):2359–2367

    Article  CAS  Google Scholar 

  • Kwon SW, Kim BY, Song J, Weon HY, Schumann P, Tindall BJ, Stackebrandt E, Fritze D (2007) Sporosarcina koreensis sp. nov. and Sporosarcina soli sp. nov., isolated from soil in Korea. Int J Syst Evol Microbiol 57(8):1694–1698

    Article  CAS  PubMed  Google Scholar 

  • Larkin JM, Stokes JL (1967) Taxonomy of psychrophilic strains of Bacillus. J Bacteriol 94(4):889–895

    CAS  PubMed Central  PubMed  Google Scholar 

  • Leifson E (1960) Atlas of Bacterial Flagellation. Academic Press, London

    Google Scholar 

  • Li WJ, Xu LH, Liu ZH, Jiang CL (2007a) Actinomycete systematic—principle, methods and practice. Science press, Beijing

    Google Scholar 

  • Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007b) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57(7):1424–1428

    Article  PubMed  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39(2):159–167

    Article  CAS  Google Scholar 

  • Minnikin D, Collins M, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Microbiol 47(1):87–95

    CAS  Google Scholar 

  • Nakamura LK (1984) Bacillus psychrophilus sp. nov., nom. rev. Int J Syst Bacteriol 34:121–123

    Article  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Tang SK, Wang Y, Chen Y, Lou K, Cao LL, Xu LH, Li WJ (2009) Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol 59(8):2025–2031

    Article  CAS  PubMed  Google Scholar 

  • Tominaga T, An SY, Oyaizu H, Yokota A (2009) Sporosarcina luteola sp. nov. isolated from soy sauce production equipment in Japan. J Gen Appl Microbiol 55(3):217–223

    Article  CAS  PubMed  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Truper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Wolfgang WJ, Coorevits A, Cole JA, De Vos P, Dickinson MC, Hannett GE, Jose R, Nazarian EJ, Schumann P, Van Landschoot A, Wirth SE, Musser KA (2012) Sporosarcina newyorkensis sp. nov. from clinical specimens and raw cow’s milk. Int J Syst Evol Microbiol 62(2):322–329

    Article  PubMed  Google Scholar 

  • Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu LH, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55(3):1149–1153

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Lee KC, Weiss N, Kho YH, Kang KH, Park YH (2001) Sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in Korea, and transfer of Bacillus globisporus (Larkin and Stokes 1967), Bacillus psychrophilus (Nakamura 1984) and Bacillus pasteurii (Chester 1898) to the genus Sporosarcina as Sporosarcina globispora comb. nov., Sporosarcina psychrophila comb. nov. and Sporosarcina pasteurii comb. nov., and emended description of th. Int J Syst Evol Microbiol 51(3):1079–1086

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from COMRA program (No. DY125-15-R-01), National Natural Science Foundation of China (41206160 and 312700040) and Scientific Research Foundation of Third Institute of Oceanography, SOA (2011002).

Author information

Authors and Affiliations

  1. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, P.R China

    Gaiyun Zhang, Huihui Ren, Yubian Zhang, Yanliu Yang & Shuang Wang

  2. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, China

    Xiu Chen & Yi Jiang

Authors
  1. Gaiyun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Huihui Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yubian Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yanliu Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shuang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yi Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gaiyun Zhang.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 285 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, G., Ren, H., Chen, X. et al. Sporosarcina siberiensis sp. nov., isolated from the East Siberian Sea. Antonie van Leeuwenhoek 106, 489–495 (2014). https://doi.org/10.1007/s10482-014-0217-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-014-0217-1

Keywords

Search

Navigation