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Effects of low-power laser irradiation (LPLI) at different wavelengths and doses on oxidative stress and fibrogenesis parameters in an animal model of wound healing

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Abstract

Gallium-arsenide (GaAs) and helium-neon (HeNe) lasers are the most commonly used low-energy lasers in physiotherapy for promoting wound healing and pain modulation. The aim of this study was investigate the effect of low-power laser irradiation (LPLI) at different wavelengths and doses on oxidative stress and fibrogenesis parameters in an animal model of wound healing. The animals were randomly divided into five groups (n = 6): Controls (skin injured animals without local or systemic treatment), skin injury treated with HeNe 1 J/cm2 (two seg); skin injury treated with HeNe 3 J/cm2 (six seg); skin injury treated with GaAs 1 J/cm2 (three seg); skin injury treated with GaAs 3 J/cm2 (nine seg). A single circular wound measuring 8 mm in diameter was surgically created on the back of the animal. The rats were irradiated at 2, 12, 24, 48, 72, 96, and 120 h after skin injury. The parameters, namely hydroxyproline content, activities of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), and lipid (TBARS) and protein oxidation (carbonyl groups) measurements were assessed. In addition, wound size regression was also analyzed. The results showed an improvement in the wound healing reflected by the reduction in wound size and increased collagen synthesis. Moreover, a significant reduction in TBARS levels, carbonyl content, and SOD and CAT activities were observed after laser irradiation, particularly with the treatments HeNe laser 1 and 3 J/cm2 dose and GaAs 3 J/cm2 dose. The data strongly indicate that LPLI therapy is efficient in accelerating the skin wound healing process after wounding, probably by reducing the inflammatory phase and inducing collagen synthesis.

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Authors and Affiliations

  1. Exercise Biochemistry and Physiology Laboratory, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, SC, Brazil

    Paulo Cesar Lock Silveira, Luciano A. Silva, Tiago P. Freitas & Ricardo A. Pinho

  2. Bioenergetics and Oxidative Stress Laboratory, Department of Biochemistry, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil

    Paulo Cesar Lock Silveira & Alexandra Latini

  3. Exercise Biochemistry and Physiology Laboratory/UNESC, Av. Universitária, 1105 – Bairro Universitário, 88806-000, Criciúma, SC, Brazil

    Paulo Cesar Lock Silveira

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  1. Paulo Cesar Lock Silveira
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  2. Luciano A. Silva
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Correspondence to Paulo Cesar Lock Silveira.

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Silveira, P.C.L., Silva, L.A., Freitas, T.P. et al. Effects of low-power laser irradiation (LPLI) at different wavelengths and doses on oxidative stress and fibrogenesis parameters in an animal model of wound healing. Lasers Med Sci 26, 125–131 (2011). https://doi.org/10.1007/s10103-010-0839-0

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  • DOI: https://doi.org/10.1007/s10103-010-0839-0

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