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The effect of combined curcumin-mediated photodynamic therapy and artificial skin on Staphylococcus aureus–infected wounds in rats

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Abstract

Healing wounds represent a major public health problem, mainly when it is infected. Besides that, the antibiotics misuse and overuse favor the development of bacterial resistance. This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) combined with artificial skin on disinfection of infected skin wound in rats. Twenty-four Wistar rats were randomly distributed into 4 groups (n = 6): (i) control—untreated; (ii) aPDT—treated with curcumin-mediated aPDT (blue light); (iii) artificial skin—treated with artificial skin alcohol-based; and (iv) aPDT plus artificial skin—treated with aPDT associated with artificial skin alcohol-based. For the in vivo model, a full-thickness biopsy with 0.80 cm was performed in order to inoculate the microorganism Staphylococcus aureus (ATCC 25923). The aPDT was performed with a curcumin gel and a blue LED light (450 nm, 80 mW/cm2) at the dose of 60 J/cm2 and the treatment with alcohol-based artificial skin was done with the topical application of 250 μL. Additional animals were submitted to aPDT combined with the artificial skin. After treatments, the number of colony-forming units (CFU) and the damage area were determined. Data were analyzed by two-way repeated measures ANOVA and Tukey tests. The highest reduction of the bacterial viability was observed in the PDT plus artificial skin group (4.14 log10), followed by artificial skin (2.38 log10) and PDT (2.22 log10) groups. In addition, all treated groups showed higher relative area of wound contraction (36.21% for the PDT, 38.41% for artificial skin, and 35.02% for PDT plus artificial) in comparison with the control group. These findings provide evidence for the positive benefits of aPDT with blue light and curcumin associated with artificial skin to decontaminate and accelerate the wound contraction.

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Acknowledgments

We would like to congratulate Mr. Rubens Janota, the inventor of the artificial skin alcohols-based.

Funding

This study was financed in part by the Foundation for the Coordination and Improvement of Higher Level or Education Personnel—Brazil (CAPES)—grant no 88887.302709/2018-00 and the São Paulo Research Foundation (FAPESP)—grant nos. 2013/14001-9 and 2013/07276-1 (CEPOF-CEPID Program).

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Authors and Affiliations

  1. School of Physical Education, State University of Minas Gerais (UEMG), R. Colorado, 700 - Bairro São Francisco, Passos, MG, CEP 37902-092, Brazil

    Fernanda Rossi Paolillo

  2. Motricity Science Institute, Rehabilitation Science Program from Federal University of Alfenas (UNIFAL), Av. Jovino Fernandes Sales, 2600 - Santa Clara, Alfenas, MG, CEP: 37133-840, Brazil

    Fernanda Rossi Paolillo

  3. Optics Group from Physics Institute of São Carlos (IFSC), University of São Paulo (USP), Av. Trabalhador Sãocarlense, 400 – Centro, São Carlos, SP, CEP 13560-970, Brazil

    Fernanda Rossi Paolillo, Phamilla Gracielli Sousa Rodrigues, Vanderlei Salvador Bagnato & Fernanda Alves

  4. Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network, Toronto, ON, M5G 2C4, Canada

    Layla Pires

  5. Medical School, Federal University of Mato Grosso do Sul, Cap. Olinto Mancini Avenue, 1662 - Colinos, Três Lagoas, MS, CEP 79600-080, Brazil

    Adalberto Vieira Corazza

Authors
  1. Fernanda Rossi Paolillo
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  2. Phamilla Gracielli Sousa Rodrigues
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  3. Vanderlei Salvador Bagnato
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  4. Fernanda Alves
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  6. Adalberto Vieira Corazza
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Corresponding author

Correspondence to Fernanda Rossi Paolillo.

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The authors declare that they have no conflict of interest.

Ethical approval

This study was approved by the Ethics and Research Committee of the Federal University of São Carlos (N. 053/2012). All animal procedures were performed in accordance with the principles in the Guide for the Care and Use of Laboratory Animals.

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Paolillo, F.R., Rodrigues, P.G.S., Bagnato, V.S. et al. The effect of combined curcumin-mediated photodynamic therapy and artificial skin on Staphylococcus aureus–infected wounds in rats. Lasers Med Sci 36, 1219–1226 (2021). https://doi.org/10.1007/s10103-020-03160-6

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  • DOI: https://doi.org/10.1007/s10103-020-03160-6

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