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Evaluation of the Efficiency of Chitosan Hydrogel Containing Berberis integerrima Root Extract on a Full-Thickness Skin Wound in a Rat Model

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Abstract

Some species of the Berberidaceae family and natural biopolymer chitosan have good effects on wound healing. In this research, a new formulation of chitosan hydrogel containing alcoholic root extract of Berberis integerrima (Chi-BIE) was designed and evaluated for the treatment of full-thickness skin wounds in an animal model. Alcoholic extract of Berberis integerrima root (BIE) was prepared, and its total amount of berberine was determined using UV/visible spectroscopy. Chitosan gel containing 10 and 20% BIE was prepared, and their extract release profile was assayed. The antimicrobial activity of Chi-BIE was investigated by the minimum inhibitory concentration assay. Finally, the efficiency of Chi-BIE formulations on the full-thickness wound healing was physically and histologically evaluated in an animal model. BIE was successfully loaded into the chitosan hydrogel and showed a burst release of extract during the first 4 h (60%) followed by a sustained release. Formulation of BIE in chitosan hydrogel could increase the antimicrobial activity of chitosan and BIE alone against Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilius, Escherichia coli, and Candida albicans. Chi-20% BIE indicated more antimicrobial activity compared to Chi-10%BIE. Formulation of BIE in chitosan hydrogel could increase the wound healing effects of chitosan and BIE alone. Chi-20% BIE had a faster restorative effect than other treatment groups due to more formation of epithelial tissue and faster maturation of bud tissue. Based on the results of this study, the chitosan hydrogel containing BIE is proposed as a suitable dressing for wound-healing.

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Author information

Author notes

  1. Maryam Hashemi and Fatemeh Kalalinia contributed equally to this work.

Authors and Affiliations

  1. Departments of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Maryam Hashemi & Fatemeh Kalalinia

  2. Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Maryam Hashemi

  3. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Fatemeh Kalalinia, Bibi Sedigheh Fazly Bazzaz & Mehrdad Iranshahi

  4. Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Mobina Razi, Fatemeh Moameri & Jebrail Movaffagh

  5. Pharmaceutical Control Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Bibi Sedigheh Fazly Bazzaz

  6. Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Mehrdad Iranshahi

  7. Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Jebrail Movaffagh

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  1. Maryam Hashemi
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Correspondence to Jebrail Movaffagh.

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Acknowledgment: The authors would like to express their sincere grateful to the Research Council of Mashhad University of Medical Sciences, Mashhad, Iran, for approval and financial support of this project. The results described in this paper were part of Pharm.D thesis of Mobina Razi.

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Hashemi, M., Kalalinia, F., Razi, M. et al. Evaluation of the Efficiency of Chitosan Hydrogel Containing Berberis integerrima Root Extract on a Full-Thickness Skin Wound in a Rat Model. Macromol. Res. 30, 527–535 (2022). https://doi.org/10.1007/s13233-022-0043-7

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  • DOI: https://doi.org/10.1007/s13233-022-0043-7

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