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Photobiomodulation of red and green lights in the repair process of third-degree skin burns

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Abstract

The aim of this study was to evaluate the photobiomodulation of red and green lights in the repair process of third-degree skin burns in rats through clinicopathological and immunohistochemical parameters. Sixty male Wistar rats were divided into three groups: control (CTRL) (n = 20), red LED (RED) (n = 20), and green LED (GREEN) (n = 20), with subgroups (n = 5) for each time of euthanasia (7, 14, 21, and 28 days). Daily applications in RED (λ630 ± 10 nm, 300 mW) and GREEN groups (λ520 ± 30 nm, 180 mW) were performed at four points of the wound (total 36 J/cm2 in RED and 240 J/cm2 in GREEN). After euthanasia, the wound retraction index (WRI) was evaluated. In histological sections, the re-epithelialization degree, the angiogenic index (AI), and the amount of myofibroblasts in wounds were analyzed. At 14 and 21 days, the RED group induced higher re-epithelialization and WRI compared to CTRL (p > 0.05) and GREEN groups (p < 0.05). At 7 and 14 days, greater AI were observed in the GREEN group, with significant difference in relation to CTRL group at 7 days (p < 0.05). At 21 and 28 days, a trend was observed for greater amount of myofibroblasts in the GREEN group, with significant difference in relation to CTRL group at 21 days (p < 0.05). The results suggest greater potential of the green light to stimulate angiogenesis in the initial periods and myofibroblastic differentiation in the final periods of the repair of third-degree skin burns. Red light may stimulate further re-epithelialization and wound retraction, especially in advanced repair phases.

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Acknowledgments

The authors acknowledge UniFacisa (Campina Grande-PB) for having authorized the development of part of this research in its animal facilities and the Coordination for the Improvement of Higher Education Personnel (CAPES) for granting a postgraduate scholarship. CFWN is research fellow at CNPq.

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Authors and Affiliations

  1. Postgraduate Program in Dentistry, State University of Paraiba, R. Baraúnas, 531, Bodocongó, Campina Grande, PB, 58429-500, Brazil

    Thamyres Maria Silva Simões, José de Alencar Fernandes Neto, Cassiano Francisco Weege Nonaka & Maria Helena Chaves de Vasconcelos Catão

  2. Medical Sciences Faculty, University Center UniFacisa, Av. Sen. Argemiro de Figueiredo, 1901, Itararé, Campina Grande, PB, 58411-020, Brazil

    Tharcia Kiara Beserra de Oliveira

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  1. Thamyres Maria Silva Simões
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  2. José de Alencar Fernandes Neto
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  3. Tharcia Kiara Beserra de Oliveira
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  4. Cassiano Francisco Weege Nonaka
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  5. Maria Helena Chaves de Vasconcelos Catão
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Correspondence to Maria Helena Chaves de Vasconcelos Catão.

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This research was approved by the Ethics Committee in the Use of Animals (CEUA) of the Center of Higher Education and Development (CESED) of Campina Grande-PB (protocol number: 6809092016).

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Simões, T.M.S., Fernandes Neto, J.d., de Oliveira, T.K.B. et al. Photobiomodulation of red and green lights in the repair process of third-degree skin burns. Lasers Med Sci 35, 51–61 (2020). https://doi.org/10.1007/s10103-019-02776-7

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