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A novel tenuivirus infecting wheat in Brazil

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Abstract

Since 1948, pale yellow wheat spike have been reported in southern Brazil. This symptom was associated with tenuiviruses due to the observation of cytoplasmic inclusions constituted by a mass of filamentous particles (7–10 nm in diameter) with indeterminate length, identical to those found in “leaf dip” preparations. Such symptoms are still seen in wheat crops; however, there is a lack of information regarding this pathosystem. Decades after the first report, the first sequences of wheat white spike virus were characterized. Wheat plants with symptoms such as pale yellowing, chlorotic streaks, and leaf mosaic were collected in Paraná State, Southern Brazil. High-throughput sequencing was used to determine the nearly complete nucleotide sequence of the viral genome. The genome is composed of five RNAs with a total size of 18,129 nucleotides, with eight open reading frames (ORFs). The virus identified in this study can be included in a new species in the family Phenuiviridae, genus Tenuivirus, and we have tentatively named this virus “wheat white spike virus”.

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Acknowledgements

The authors would like to thank Professor Murilo Zerbini for providing reagents and support in RACE analysis. This work was supported by EMBRAPA (project 32.16.04.037.00.00: Viral population analysis and management strategies for the common mosaic in wheat in Brazil), Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina (FAPESC – PAP) (Grant number: PAP2018), and UDESC. F.S.P. and S.C.N are Brazilian Federation for Support and Evaluation of Graduate Education (CAPES) fellows.

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Authors and Affiliations

  1. Universidade do Estado de Santa Catarina, Lages, SC, Brazil

    Fernando Sartori Pereira, Samara Campos do Nascimento, Amauri Bogo, Ricardo Trezzi Casa & Fabio Nascimento da Silva

  2. Universidade Federal de Viçosa, Viçosa, MG, Brazil

    Lucas Antonio Stempkowski

  3. Embrapa Uva e Vinho, Bento Gonçalves, RS, Brazil

    Thor Vinícius Martins Fajardo

  4. Embrapa Agricultura Digital, Campinas, SP, Brazil

    Antonio Nhani Júnior

  5. Embrapa Trigo, Passo Fundo, RS, Brazil

    Douglas Lau & Talita Bernardon Mar

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  1. Fernando Sartori Pereira
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  2. Lucas Antonio Stempkowski
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  3. Thor Vinícius Martins Fajardo
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  4. Antonio Nhani Júnior
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  5. Douglas Lau
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  6. Talita Bernardon Mar
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  7. Samara Campos do Nascimento
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  8. Amauri Bogo
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  9. Ricardo Trezzi Casa
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  10. Fabio Nascimento da Silva
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Corresponding author

Correspondence to Fabio Nascimento da Silva.

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None of the authors have a conflict of interest. This article does not contain any studies with animals performed by any of the authors.

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Pereira, F.S., Stempkowski, L.A., Fajardo, T.V.M. et al. A novel tenuivirus infecting wheat in Brazil. Arch Virol 167, 989–993 (2022). https://doi.org/10.1007/s00705-022-05361-6

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  • DOI: https://doi.org/10.1007/s00705-022-05361-6

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