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Molecular characterization of a novel wheat-infecting virus of the family Betaflexiviridae

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Abstract

Wheat plants showing yellowing and mosaic in leaves and stunting were collected from wheat fields in Henan Province, China. Analysis of these plants by transmission electron microscopy showed that they contained two types of filamentous virus-like particles with a length of 200-500 nm and 1000-1300 nm, respectively. RNA-seq revealed a coinfection with wheat yellow mosaic virus (WYMV) and an unknown wheat-infecting virus. The genome of the unknown virus is 8,410 nucleotides long, excluding its 3’ poly(A) tail. It has six open reading frames (ORFs). ORF1 encodes a putative viral replication-associated protein (Rep), and ORFs 2, 3, and 4 encode the triple gene block (TGB) proteins. ORFs 5 and 6 encode the capsid protein (CP) and a protein with unknown function, respectively. Phylogenetic analysis showed that this novel virus is evolutionarily related to members of the subfamily Quinvirinae, family Betaflexiviridae. It is, however, distinct from the viruses in the currently established genera. Based on the species and genus demarcation criteria set by the International Committee on Taxonomy of Viruses (ICTV), we tentatively name this novel virus "wheat yellow stunt-associated betaflexivirus" (WYSaBV), and we propose it to be a member of a new genus in the family Betaflexiviridae.

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Acknowledgements

This research was supported by grants from the National Natural Science Foundation of China for Youth Scholars (31801701) and the China Postdoctoral Science Foundation (2019T120521 and 2018M642449). We thank Dr. Xinshun Ding (retired, New York, USA) for his help on the language of this manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China

    Shuai Fu, Tianze Zhang, Mengzhu He, Xueping Zhou & Jianxiang Wu

  2. College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, China

    Bingjian Sun

  3. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Xueping Zhou

Authors
  1. Shuai Fu
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  2. Tianze Zhang
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  3. Mengzhu He
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  4. Bingjian Sun
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  5. Xueping Zhou
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  6. Jianxiang Wu
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Contributions

JW designed the experiments; SF, MH, and BS performed the experiments; TZ performed the bioinformatics analysis; JW, XZ, and SF wrote the manuscript.

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Correspondence to Jianxiang Wu.

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All authors declare they have no conflict of interest.

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Fu, S., Zhang, T., He, M. et al. Molecular characterization of a novel wheat-infecting virus of the family Betaflexiviridae. Arch Virol 166, 2875–2879 (2021). https://doi.org/10.1007/s00705-021-05175-y

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  • DOI: https://doi.org/10.1007/s00705-021-05175-y

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