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Complete genome sequence of a novel mitovirus detected in Paris polyphylla var. yunnanensis

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Abstract

Paris mitovirus 1 (ParMV1) is a positive-sense RNA virus that was detected in diseased Paris polyphylla var. yunnanensis plants in Wenshan, Yunnan. The complete genome sequence of ParMV1 is 2,751 nucleotides in length, and the genome structure is typical of mitoviruses. The ParMV1 genome has a single open reading frame (ORF; nt 358-2,637) that encodes an RNA-dependent RNA polymerase (RdRp) with a predicted molecular mass of 86.42 kDa. ParMV1 contains six conserved motifs (Ι-VΙ) that are unique to mitoviruses. The 5′ and 3′ termini of the genome are predicted to have a stable secondary structure, with the reverse complementary sequence forming a panhandle structure. Comparative genome analysis revealed that the RdRp of ParMV1 shares 23.1-40.6% amino acid (aa) and 32.3-45.7% nucleotide (nt) sequence identity with those of other mitoviruses. Phylogenetic analysis based on RdRp aa sequences showed that ParMV1 clusters with mitoviruses and hence should be considered a new member of the genus Mitovirus in the family Mitoviridae. This is the first report of a novel mitovirus infecting Paris polyphylla var. yunnanensis.

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Data availability

The dataset generated during the current study is available in the GenBank database (accession no. MT269666).

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Funding

This study was funded by the National Natural Science Foundation of China (81860774).

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Author notes

  1. Zeli Chen and Lu Chen contributed equally to this study.

Authors and Affiliations

  1. Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Zeli Chen, Lu Chen, Zhe Wang, Like Gao, Shangyun Li & Mingfu Zhao

  2. College of Agronomy and Biotechnology, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Zeli Chen, Shangyun Li & Guosong Wen

  3. Research & Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, 32 Jiaochang Donglu, Kunming, 650201, Yunnan, China

    Zeli Chen, Lu Chen, Zhe Wang, Like Gao, Shangyun Li, Guosong Wen & Mingfu Zhao

  4. Center for Excellence in Animal Evolution and Genetics, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, 650223, Yunnan, China

    Rex Frimpong Anane

  5. State Key Laboratory for Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, 650223, Yunnan, China

    Rex Frimpong Anane

  6. Plant Protection and Quarantine Station of Yuanjiang County, Yuxi, 653300, Yunnan, China

    Daihong Yu

Authors
  1. Zeli Chen
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  2. Lu Chen
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  3. Rex Frimpong Anane
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  4. Zhe Wang
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  8. Daihong Yu
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  9. Mingfu Zhao
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Corresponding author

Correspondence to Mingfu Zhao.

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Chen, Z., Chen, L., Anane, R.F. et al. Complete genome sequence of a novel mitovirus detected in Paris polyphylla var. yunnanensis. Arch Virol 167, 645–650 (2022). https://doi.org/10.1007/s00705-021-05339-w

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  • DOI: https://doi.org/10.1007/s00705-021-05339-w

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