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Complete genome sequence of a novel mitovirus from the ectomycorrhizal fungus Geopora sumneriana

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Abstract

A double-stranded RNA (dsRNA) segment was extracted from the ectomycorrhizal fungus Geopora sumneriana (Cooke) M. Torre, and its full-length cDNA sequence, comprising 3146 nucleotides, was determined. Sequence analysis revealed the presence of a large open reading frame (ORF) on the positive strand of this dsRNA segment when the mold mitochondrial genetic code was applied. The ORF encodes a putative RNA-dependent RNA polymerase (RdRp), which shares the highest degree of similarity with Tuber excavatum mitovirus, with 37.52% identity. This dsRNA segment represents the genome replication intermediate of a novel mitovirus that was tentatively designated as “Geopora sumneriana mitovirus 1” (GsMV1). Phylogenetic analysis further suggested that GsMV1 is a member of the family Narnaviridae. This is the first study reporting on a mitovirus genome sequence in the ectomycorrhizal fungus G. sumneriana.

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Acknowledgements

We are grateful to Dr. Aykut Ozkul and his research team for their valuable comments and moral support.

Funding

This study was financially supported by the Ankara University Research Funding Unit with the project numbers 18B0430001 and 19B0430002.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Ankara University, Tandogan, 06100, Ankara, Turkey

    Ergin Sahin & Ilgaz Akata

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  1. Ergin Sahin
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  2. Ilgaz Akata
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Correspondence to Ergin Sahin.

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Sahin, E., Akata, I. Complete genome sequence of a novel mitovirus from the ectomycorrhizal fungus Geopora sumneriana. Arch Virol 164, 2853–2857 (2019). https://doi.org/10.1007/s00705-019-04367-x

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  • DOI: https://doi.org/10.1007/s00705-019-04367-x

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