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The titers of rice tungro bacilliform virus dictate the expression levels of genes related to cell wall dynamics in rice plants affected by tungro disease

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Abstract

Rice tungro disease (RTD) is a devastating disease of rice caused by combined infection with rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV), with one of the main symptoms being stunting. To dissect the molecular events responsible for RTD-induced stunting, the expression patterns of 23 cell-wall-related genes were examined in different rice lines with the same titers of RTSV but different titers of RTBV and in lines where only RTBV was present. Genes encoding cellulose synthases, expansins, glycosyl hydrolases, exostosins, and xyloglucan galactosyl transferase showed downregulation, whereas those encoding defensin or defensin-like proteins showed upregulation with increasing titers of RTBV. RTSV titers did not affect the expression levels of these genes. A similar relationship was seen for the reduction in the cellulose and pectin content and the accumulation of lignin. In silico analysis of promoters of the genes indicated a possible link to transcription factors reported earlier to respond to viral titers in rice. These results suggest a common network in which the genes related to the cell wall components are affected during infection with diverse viruses in rice.

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Acknowledgements

GK acknowledges a research associate fellowship funded by the Council of Scientific and Industrial Research, New Delhi. Funds for this work were made available as the J. C. Bose Fellowship by the Science and Engineering Research Board, Department of Science and Technology, Government of India, to ID. The authors acknowledge a DU-DST FIST infrastructural grant of the Department of Plant Molecular Biology, University of Delhi South Campus

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  1. Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India

    Gaurav Kumar & Indranil Dasgupta

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  1. Gaurav Kumar
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The work was conceived by GK and ID, GK performed the experiments and analyses, and GK and ID wrote and approved the manuscript.

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Correspondence to Indranil Dasgupta.

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Kumar, G., Dasgupta, I. The titers of rice tungro bacilliform virus dictate the expression levels of genes related to cell wall dynamics in rice plants affected by tungro disease. Arch Virol 166, 1325–1336 (2021). https://doi.org/10.1007/s00705-021-05006-0

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