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Novel Caulobacter bacteriophages illustrate the diversity of the podovirus genus Rauchvirus

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Abstract

The podovirus BPP-1 is currently the only member of the Podovirus genus Rauchvirus. Here, we describe three new Caulobacter bacteriophages (Jess A, SR18, and RW) that show genetic similarity to BPP-1 but have many different genetic and structural features that differentiate them from BPP-1. Jess A and SR18 are closely related to each other and should be considered two members of a new species. They share a similar gene order with BPP-1. However, they do not appear to form lysogens or have the tropism switching mechanism that has been described for BPP-1. Bacteriophage RW also exhibits some homology to BPP-1. However, it is quite different from the other three phages, and we propose that it should be considered a representative of a third species of the genus Rauchvirus. Taken together, the differences among these four members of the genus Rauchvirus indicate that this divergent genus has a long evolutionary history and that there are many more rauchviruses waiting to be discovered.

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Funding

This work was funded in part by National Institutes of Health grant GM076277 to BE.

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Author notes

  1. Doreen Nguyen

    Present address: Department of Microbiology, University of Georgia, Athens, USA

  2. Kiesha Wilson

    Present address: Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, USA

Authors and Affiliations

  1. Department of Biological Sciences, University of South Carolina, Columbia, SC, 29208, USA

    Bert Ely, Jana Liese, Scott Corley, Doreen Nguyen, Kiesha Wilson & Louis Berrios

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  1. Bert Ely
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  2. Jana Liese
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  3. Scott Corley
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  4. Doreen Nguyen
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  6. Louis Berrios
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Correspondence to Bert Ely.

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Ely, B., Liese, J., Corley, S. et al. Novel Caulobacter bacteriophages illustrate the diversity of the podovirus genus Rauchvirus. Arch Virol 165, 2549–2554 (2020). https://doi.org/10.1007/s00705-020-04791-4

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  • DOI: https://doi.org/10.1007/s00705-020-04791-4

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