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High-performance colorimetric immunoassay for determination of chloramphenicol using metal–organic framework-based hybrid composites with increased peroxidase activity

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Abstract

Metal–organic frameworks (MOFs) as carriers for high-capacity loading of HRP-IgG and gold nanoparticles are introduced, to prepare MOF hybrids with enhanced peroxidase activity. The prepared MOF hybrids were employed to establish an indirect competitive colorimetric immunoassay for chloramphenicol (CAP) detection, in which the limit of detection for CAP is 0.006 μg·L−1, only one-fifth of that of the conventional ELISA using the same antibodies and antigens. The linear range was 0.008–0.108 μg·L−1, and the recovery of spiked milk samples varied in the range 76.0–106.0% through three independent experiments. Our proposed colorimetric immunoassay using the MOF hybrid immunoprobe provides a novel platform for ultra-sensitive determination of CAP residues, and it also could be used as a signal amplification model for the high-performance colorimetric immunoassay in food safety monitoring.

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Acknowledgements

This work was financially supported by funding from the National Natural Science Foundation of China (31672605), Natural Science Foundation of Chongqing, China, grant number (cstc2019jcyj-msxmX0314, cstc2018jcyjAX0242), Zhejiang Natural Science Foundation, grant number (LGN18C200026), and The innovation platform for Academicians of Hainan Province.

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Authors and Affiliations

  1. College of Food Science, Southwest University, Chongqing, 400715, People’s Republic of China

    Xin Wang & Xiaoqi Tao

  2. Institute for Clean Energy & Advanced Materials, School of Materials & Energy, Southwest University, Chongqing, 400715, People’s Republic of China

    Zhisong Lu

  3. Key Laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Province, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, 571158, People’s Republic of China

    Wei Sun

  4. Zhejiang Institute of Economics and Trade, Hangzhou, 310018, People’s Republic of China

    Sudan Ye

  5. Chongqing Key Laboratory of Speciality Food Co-Built By Sichuan and Chongqing, Chongqing, 400715, People’s Republic of China

    Xiaoqi Tao

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  1. Xin Wang
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  3. Wei Sun
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  4. Sudan Ye
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Contributions

Xin Wang: experiment, writing original draft. Wei Sun: software, data curation. Sudan Ye: software, data curation. Zhisong Lu: methodology, conceptualization; funding acquisition, review, supervision. Xiaoqi Tao: conceptualization; review & editing, funding acquisition, supervision.

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Correspondence to Zhisong Lu or Xiaoqi Tao.

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Wang, X., Lu, Z., Sun, W. et al. High-performance colorimetric immunoassay for determination of chloramphenicol using metal–organic framework-based hybrid composites with increased peroxidase activity. Microchim Acta 189, 484 (2022). https://doi.org/10.1007/s00604-022-05586-x

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