Abstract
A multi pathogenic microorganisms determination method is reported using DNA composites encapsulated DNA silver nanocluster (AgNCs)/graphene oxide (GO)-based system through rolling cycle (RCA) amplification. Firstly, two different RCA-based DNA composites are assembled, coupled with thousands of DNA-stabilized AgNCs probe and ssDNA aptamer specific for two pathogen bacteria targets. GO was then introduced into the system to capture ssDNA aptamer of DNA composites and as a selective fluorescence quencher of DNA/AgNCs. Upon recognizing the target bacteria, ssDNA aptamer part would combine with bacteria and release from the surface of GO. Thus, DNA/AgNCs of RCA-based DNA composites can generate strong fluorescence signal. With the fluorescent report of RCADNA-AgNCs/530 and RCADNA-AgNCs/625, the assay successfully detect Escherichia coli and Staphylococcus aureus at concentrations as low as 38 CFU/mL, and a highly selective and efficient sensing platform was achieved. Therefore, this RCA/DNA-AgNCs/GO-based platform shows excellent application in multi pathogenic microorganisms determination and potential clinic therapy.
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Funding
The study was funded by National Natural Science Foundation of China (41906037, and 41876101), and Nantong Scientific Plan Foundation (JC2020090 and JC2021054), Open fund of Wenzhou Key Laboratory of Sanitary Microbiology (ZD202003KF01), and Shandong Provincial Marine Science and Technology Projects of 2018.
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Zeng, Y., Qi, P., Zhou, Y. et al. Multi pathogenic microorganisms determination using DNA composites-encapsulated DNA silver nanocluster/graphene oxide-based system through rolling cycle amplification. Microchim Acta 189, 403 (2022). https://doi.org/10.1007/s00604-022-05505-0
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DOI: https://doi.org/10.1007/s00604-022-05505-0