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One-pot green preparation of deep-ultraviolet and dual-emission carbon nanodots for dual-channel ratiometric determination of polyphenol in tea sample

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Abstract

A novel deep-ultraviolet and dual-emission carbon nanodots (DUCDs)-based dual-channel ratiometric probe was prepared by a one-pot environmental-friendly hydrothermal process using guanidine as the only starting material for sensing polyphenol in tea sample (TPPs). Under the exposure to TPPs, the DUCDs not only provided a characteristic colorimetric response to TPPs, but also displayed TPPs-sensitive ratiometric fluorescence quenching. The detection mechanism was proved to be that enrichment-specific hydroxyl sites (e.g., -NH2 and -COOH) of DUCDs can specifically react with phenolic hydroxyl groups of TPPs to generate dynamic amide and carboxylate bonds by dehydration and/or condensation reaction. As a result, a new carbon nanomaterial with decrement of surface passivation groups, inherent light-absorbing, and invalid fluorescence emission was generated. The ratio (FL297nm/FL395nm) of fluorescence intensity at 297 nm and 395 nm of DUCDs excited at 275 nm decreased with increasing TPPs concentration. The linearity range was 5.0 ng/mL to 100 µg/mL with a detection limit (DL) of 3.5 ± 0.04 ng/mL for TPPs (n = 3, 3σ/k). Colorimetry of DUCDs, best measured as absorbance at 320 nm, was increased linearly in the TPP concentration range 200 ng/mL–200 µg/mL with a DL of 94.7 ± 0.04 ng/mL (n = 3, 3σ/k). The probe was successfully applied to the determination of TPPs in real tea samples, showing potential application prospects in food analysis.

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Funding

Financial support for this work was provided by the National Natural Science Foundation of China (21705140, 21876144, 21974120), and the Natural Science Foundation of Jiangsu Province (BK20170474).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, 224051, China

    Zhong-Xia Wang, Lei Hu, Wen-Juan Wang, Fen-Ying Kong, Mei-Jie Wei, Hai-Lin Fang & Wei Wang

  2. School of Science, Jiangsu Ocean University, Lianyungang, 222005, China

    Qi-Le Li

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Wang, ZX., Hu, L., Wang, WJ. et al. One-pot green preparation of deep-ultraviolet and dual-emission carbon nanodots for dual-channel ratiometric determination of polyphenol in tea sample. Microchim Acta 189, 241 (2022). https://doi.org/10.1007/s00604-022-05330-5

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