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Peroxidase-mimetic activity of a nanozyme with uniformly dispersed Fe3O4 NPs supported by mesoporous graphitized carbon for determination of glucose

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Abstract

A Fe3O4/mesoporous graphitized carbon (Fe3O4/m-GC) composite was prepared through a facile calcination method with iron-based metal–organic frameworks (Fe-MOFs) as a sacrificial template. After carbonization, the Fe3O4 nanoparticles were uniformly dispersed in the mesoporous carbon support, resulting in spatial structural stability. The mesoporous carbon support obtained was highly graphitized and exhibited eminent electrical conductivity, which accelerated the electron transfer between the Fe3O4 nanoparticles by Fe(II)/Fe(III) redox cycles and m-GC by C = Csp2/C-Csp3 redox cycles, leading to the excellent peroxidase-mimetic activity of Fe3O4/m-GC. Km values for tetramethylbenzidine (TMB) and H2O2 were 26.8 and 15.8 times lower than that of natural horseradish peroxidase, respectively. Taking advantage of the peroxidase-mimetic activity of Fe3O4/m-GC, a colorimetric assay was fabricated for detecting glucose in the range 0.5 ~ 200 μM, with a limit of detection of 0.24 μM.

Graphical abstract

Fig 1 A Schematic illustration of the preparation process of Fe3O4/m-GC, B schematic illustration of a proposed synergistic catalytic mechanism of TMB oxidation by Fe3O4/m-GC.

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Funding

This work was supported by the Nature Science Foundation of Hunan (2021JJ30701), the National Natural Science Foundation of China (31401566, 31601550) and the National Key R&D Program of China (2016YFF0203701).

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Authors and Affiliations

  1. Hunan Provincial Key Laboratory of Cytochemistry, College of Chemistry and Food Engineering, Changsha University of Science & Technology, Changsha, 410114, People’s Republic of China

    Zhou Xu, Lin Li, Kai Li, Mao-Long Chen & Yun-Hui Cheng

  2. Hunan Academy of Forestry, Changsha, 410004, People’s Republic of China

    Jia Tu

  3. School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, People’s Republic of China

    Wei Chen

  4. Hunan Key Laboratory of Food Safety Science & Technology, Changsha Customs Technology Center, Changsha Customs District, Changsha, 410116, People’s Republic of China

    Shao-Hua Zhu

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  1. Zhou Xu
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Xu, Z., Li, L., Li, K. et al. Peroxidase-mimetic activity of a nanozyme with uniformly dispersed Fe3O4 NPs supported by mesoporous graphitized carbon for determination of glucose. Microchim Acta 188, 421 (2021). https://doi.org/10.1007/s00604-021-05035-1

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