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Electrochemical sensor based on dual-template molecularly imprinted polymer and nanoporous gold leaf modified electrode for simultaneous determination of dopamine and uric acid

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Abstract

A novel electrochemical sensor based on dual-template molecularly imprinted polymer (MIP) with nanoporous gold leaf (NPGL) was established for the simultaneous determination of dopamine (DA) and uric acid (UA). NPGL acts as an enlarged loading platform to enhance sensing capacity, and the MIP layer was synthesized in situ in the presence of monomer and dual templates (DA and UA) to provide specific recognition. Under the optimal conditions, the sensor shows a good linear range of 2.0~180 μM for DA at a working potential of 0.15 V (vs. Ag/AgCl) and 5.0~160 μM for UA at 0.35 V (vs. Ag/AgCl), with the respective detection limit of 0.3 μM and 0.4 μM (S/N = 3). Good selectivity of the sensor to its dual templates was confirmed as the sensing signals are significantly different between templates and interfering species. The responses maintained higher than 96% of the initial values after 30-day storage, and the day-to-day relative standard deviation is less than 3.0%. Real sample simultaneous determination of DA and UA was conducted with bovine serum, and the results were in good agreement with those from high-performance liquid chromatography. It can be concluded that this work offers a reliable, facile, fast, and cost-effective method of simultaneous quantification of two or more chem-/bio-molecules.

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Acknowledgments

The work was financially supported by Shenzhen science and technology research and development fund (JCYJ20180305180609083), Shenzhen Science and Technology Program (KQJSCX20180328165437711, KQTD20170810105439418), the project on the key technique improvement of Xinjiang Licorice planting and quality control of Xinjiang Production & Construction Corps (2018AB012), Innovative team and talent training project of Shihezi (2018TD02), and National Natural Science Foundation of China (81773680, 81973280).

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Author notes

  1. Nan Li and Chuanchuan Nan contributed equally to this work.

Authors and Affiliations

  1. Department of Stomatology Center, Shenzhen People’s Hospital (Second Clinical Medical School of Jinan University; First Affiliated Hospital of Southern University of Science and Technology), Shenzhen, 518020, Guangdong, China

    Nan Li

  2. Department of ICU, Shenzhen People’s Hospital (Second Clinical Medical School of Jinan University; First Affiliated Hospital of Southern University of Science and Technology), Shenzhen, 518020, Guangdong, China

    Chuanchuan Nan

  3. College of Science, Harbin Institute of Technology (Shenzhen), Shenzhen, 518055, Guangdong, People’s Republic of China

    Xuecui Mei, Yudong Sun & Yingchun Li

  4. Research Centre of Printed Flexible Electronics, School of Materials Science and Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen, 518055, China

    Huanhuan Feng

  5. State Key Laboratory of Advanced Welding and Joining, Harbin Institute of Technology (Shenzhen), Shenzhen, 518055, China

    Huanhuan Feng

  6. College of Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, China

    Yingchun Li

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  1. Nan Li
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Correspondence to Huanhuan Feng or Yingchun Li.

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Li, N., Nan, C., Mei, X. et al. Electrochemical sensor based on dual-template molecularly imprinted polymer and nanoporous gold leaf modified electrode for simultaneous determination of dopamine and uric acid. Microchim Acta 187, 496 (2020). https://doi.org/10.1007/s00604-020-04413-5

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