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Aptamer-involved fluorescence amplification strategy facilitated by directional enzymatic hydrolysis for bioassays based on a metal-organic framework platform: Highly selective and sensitive determination of thrombin and oxytetracycline

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Abstract

The authors describe a fluorescence amplification strategy for selective and sensitive fluorescent assays based on aptamer-triggered directional hydrolysis and on the use of metal organic frameworks (MOFs) of type MIL-101. The method is implemented by mixing MIL-101, fluorescein-labeled DNA probes, exonuclease of type RecJf, and targets. A smart design of the three-adenine bulge on the DNA probe facilitates exonuclease-assisted directional hydrolysis, making the strategy universal for determination of both proteins and small molecules as well. Good selectivity is accomplished due to the use of MIL-101 protected aptamers, while high sensitivity resulted from exonuclease-assisted target-recycling signal amplification. The power of the method is demonstrated by analyzing the two model analytes thrombin (a fairly large protein) and oxytetracycline (OTC; a small molecule antibiotic). The limits of detection are 15 pM for thrombin and 4.2 nM for OTC. This is two orders of magnitude lower than that of conventional 1:1 homogeneous fluorescence assays. The strategy was successfully applied to the analysis of thrombin and OTC in real samples. In our perception, the strategy presented here has a wide scope for selective and sensitive detection of trace analytes for which appropriate DNA probes can be identified.

Schematic of an aptamer-triggered amplification strategy for sensitive bioassays based on fluorescence quenching of a MOF and 5′→3′ directional hydrolysis of RecJf exonuclease. The adenine-bulge on the DNA probe facilitates exonuclease hydrolysis, making the strategy universal for both proteins and small molecules detection.

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Acknowledgements

The work was supported by the National Natural Science FoundationᅟofᅟChinaᅟ(Nos.ᅟ21475153,ᅟ21575167ᅟand 21675178), the Guangdong Provincial Natural Science Foundation of China (No. 2015A030311020), and the Special Funds for Public Welfare Research and Capacity Building in Guangdong Province of China (No. 2015A030401036), and the Guangzhou Science and Technology Program of China (No. 201604020165), respectively.

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  1. School of Chemistry, Sun Yat-sen University, Guangzhou, Guangdong, 510275, People’s Republic of China

    Jincan He, Gongke Li & Yuling Hu

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  1. Jincan He
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  2. Gongke Li
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Correspondence to Gongke Li or Yuling Hu.

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He, J., Li, G. & Hu, Y. Aptamer-involved fluorescence amplification strategy facilitated by directional enzymatic hydrolysis for bioassays based on a metal-organic framework platform: Highly selective and sensitive determination of thrombin and oxytetracycline. Microchim Acta 184, 2365–2373 (2017). https://doi.org/10.1007/s00604-017-2263-7

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