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A Label-free mid Turn-on Fluorescence Strategy for DNA Detection with a Wide Detection Range Based on Exonuclease III-aided Target Recycling Amplification

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Abstract

In the present work, a simple and sensitive turn-on fluorescence method for DNA detection was developed. It was explored based on the N-methyl mesoporphyrin IX (NMM)/G-quadruplex DNA system as a reporter and exonuclease III (Exo III)-aided target recycling amplification to ensure sensitivity. Our method showed an ultra-wide detection range from 10 fM to 100 nM with a low linear detection limit of 0.76 nM. It also had excellent selectivity in a selectivity experiment.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (31601536, 31401477) and the Science and Technology Research Project from Hubei Provincial Education Department (D20161303).

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Authors and Affiliations

  1. Edible and Medicinal Fungi Research Center, College of Life Science, Yangtze University, Jingzhou, Hubei, 434025, China

    Hualin Yang, Baomiao Ding, Zhenshun Li & Xingping Zhang

  2. School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu, 210009, China

    Xiaoda Song

Authors
  1. Hualin Yang
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  2. Xiaoda Song
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  3. Baomiao Ding
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  4. Zhenshun Li
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  5. Xingping Zhang
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Yang, H., Song, X., Ding, B. et al. A Label-free mid Turn-on Fluorescence Strategy for DNA Detection with a Wide Detection Range Based on Exonuclease III-aided Target Recycling Amplification. ANAL. SCI. 33, 9–11 (2017). https://doi.org/10.2116/analsci.33.9

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  • DOI: https://doi.org/10.2116/analsci.33.9

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