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The ectomycorrhizal status of a tropical black bolete, Phlebopus portentosus, assessed using mycorrhizal synthesis and isotopic analysis

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Abstract

Phlebopus portentosus is one of the most popular wild edible mushrooms in Thailand and can produce sporocarps in the culture without a host plant. However, it is still unclear whether Phlebopus portentosus is a saprotrophic, parasitic, or ectomycorrhizal (ECM) fungus. In this study, Phlebopus portentosus sporocarps were collected from northern Thailand and identified based on morphological and molecular characteristics. We combined mycorrhizal synthesis and stable isotopic analysis to investigate the trophic status of this fungus. In a greenhouse experiment, ECM-like structures were observed in Pinus kesiya at 1 year after inoculation with fungal mycelium, and the association of Phlebopus portentosus and other plant species showed superficial growth over the root surface. Fungus-colonized root tips were described morphologically and colonization confirmed by molecular methods. In stable isotope measurements, the δ13C and δ15N of natural samples of Phlebopus portentosus differed from saprotrophic fungi. Based on the isotopic patterns of Phlebopus portentosus and its ability to form ECM-like structures in greenhouse experiments, we conclude that Phlebopus portentosus could be an ECM fungus.

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Acknowledgments

This work was supported by grants from the Thailand Research Fund for The Royal Golden Jubilee Ph.D. Program (PHD/0309/2550) and Research Team Association Grant RTA5580007, and Chiang Mai University, Thailand, and grant IOS-0843366 to Erik Hobbie from the US National Science Foundation. We thank Andrew Wilson and Jesse Sadowsky for very useful comments on the manuscript.

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  1. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand

    Jaturong Kumla, Nakarin Suwannarach & Saisamorn Lumyong

  2. Earth Systems Research Center, Morse Hall, University of New Hampshire, Durham, NH, 03824, USA

    Erik A. Hobbie

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  1. Jaturong Kumla
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Kumla, J., Hobbie, E.A., Suwannarach, N. et al. The ectomycorrhizal status of a tropical black bolete, Phlebopus portentosus, assessed using mycorrhizal synthesis and isotopic analysis. Mycorrhiza 26, 333–343 (2016). https://doi.org/10.1007/s00572-015-0672-1

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