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Field application of mycorrhizal bio-inoculants affects the mineral uptake of a forage legume (Hedysarum coronarium L.) on a highly calcareous soil

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Abstract

The efficiency of two mycorrhizal bio-inoculants on the mineral uptake during the growth stages of a Mediterranean forage legume sulla (Hedysarum coronarium L.) was studied in the field on a highly calcareous soil. The first inoculum (Mm) was made up of a mixture of native arbuscular mycorrhizal fungi (AMF) isolated from calcareous soils: Septoglomus constrictum, Funneliformis geosporum, Glomus fuegianum, Rhizophagus irregularis and Glomus sp. The second was a commercial inoculum (Mi) containing one AMF species: R. irregularis. Both mycorrhizal inoculants increased total and arbuscular colonization of sulla roots. Inoculation with Mm showed a positive effect on sulla shoot dry weight (SDW) when compared to Mi and non-inoculated plants (control). Mineral contents (P, Mg, Mn, Fe) were higher in the shoots of sulla plants cultivated on mycorrhiza-inoculated plots compared to non-inoculated ones. This enhancement was observed during the flowering stage for P, Mg and Mn and during the rosette stage for Fe. An increase in P content of 50 % in plants inoculated with Mm compared to non-inoculated ones may be explained by the induction of root alkaline and acid phosphatase activities. Higher efficiency of native AMF species adapted to calcareous soils opens the way towards the development of mycorrhiza bio-fertilizers targeted to improve sustainable fertilization management in such soils.

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Acknowledgments

This work was supported by the Tunisian Ministry of High Education and Research which partially financed S. Labidi’s PhD thesis. The authors are grateful to Natacha Bourdon and Jebrane Chrigui for their technical help.

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  1. Laboratoire des Sciences Horticoles, Institut National Agronomique de Tunisie, Université de Carthage, 43 Ave Charles Nicolle, 1082, Tunis, Mahrajène, Tunisie

    S. Labidi, F. Ben Jeddi & M. Yousfi

  2. Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), EA 4492, Université Lille Nord de France, F-59000 Lille, Université du Littoral Côte d’Opale, 50 rue Ferdinand Buisson, 62228, Calais, France

    S. Labidi, B. Tisserant & A. Lounès-Hadj Sahraoui

  3. Laboratoire des Sciences du Sol et Environnement, Institut National Agronomique de Tunisie, Université de Carthage, 43 Ave Charles Nicolle, 1082, Tunis, Mahrajène, Tunisie

    M. Sanaa

  4. Centre de recherche de l’est sur les céréales et oléagineux (CRECO), 960 Ave Carling, Ottawa, K1A 0C6, Canada

    Y. Dalpé

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  1. S. Labidi
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Labidi, S., Jeddi, F.B., Tisserant, B. et al. Field application of mycorrhizal bio-inoculants affects the mineral uptake of a forage legume (Hedysarum coronarium L.) on a highly calcareous soil. Mycorrhiza 25, 297–309 (2015). https://doi.org/10.1007/s00572-014-0609-0

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