Abstract
White clover (Trifolium repens L.) plants were grown in a calcareous soil in pots with three compartments, a central one for root growth and two outer ones for growth of vesicular-arbuscular (VA) mycorrhizal (Glomus mosseae [Nicol. & Gerd.] Gerdemann & Trappe) hyphae (hyphal compartments). Phosphorus (P) was applied at three levels (0, 20 and 50 mg kg−1 soil) in the outer compartments in mycorrhizal treatments. Root and shoot dry weight were increased in mycorrhizal plants with hyphal access to outer compartments. Growth of the mycorrhizal hyphae in the outer compartments was not significantly affected by variation in P level in these compartments. However, both concentration and amount of P in roots and shoots sharply increased with increasing P supply in the outer (hyphal) compartments. With increasing P levels the calculated delivery of P by the hyphae from the outer compartments increased from 34% to 90% of total P uptake.
Hyphal access to the outer compartments also significantly increased both concentration and quantity of Cu in the plants. The calculated delivery of Cu by the hyphae from the outer compartments ranged from 53% to 62% of total Cu uptake, irrespective of the P levels and the amounts of P taken up and transported by the hyphae. However, the distribution of Cu over roots and shoots was largely dependent on P levels. With increase in P level in the outer compartments the calculated hyphal contribution to the total amount of Cu in the shoots increased from 12% to 58%, but decreased in the roots from 75% to 46%.
In conclusion, uptake and transport by VA-mycorrhizal hyphae may contribute substantially not only to P nutrition, but also to Cu nutrition of the host.
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References
Ames R N, Reid C P P, Porter L K and Cambardella C 1983 Hyphal uptake and transport of nitrogen from two15N labelled sources byGlomus mosseae, a vesicular-arbuscular mycorrhizal fungus. New Phytol. 95, 381–396.
Ashford A E, Peterson R L, Dwarte D and Chilvers G A 1986 Polyphosphate granules in eucalypt mycorrhizas: Determination by energy dispersive X-ray microanalysis. Can. J. Bot. 64, 677–687.
Bowen G D 1980 Mycorrhizal roles in tropical plants and ecosystems.In Tropical Mycorrhiza Research. Ed. P. Mikola. pp 165–190. Clarendon Press, Oxford.
Bradley R, Burt A J and Read D J 1981 Mycorrhizal infection and resistance to heavy metal toxicity inCalluna vulgaris. Nature (London) 292, 335–337.
Bradley R, Burt A J and Read D J 1982 The biology of mycorrhiza in the Ericaceae. VIII. The role of mycorrhizal infection in heavy metal resitance. New Phytol. 91, 197–209.
Buwalda J G, Stribley D P and Tinker P B 1983 Increased uptake of bromide and chloride by plants infected with vesicular-arbuscular mycorrhizas. New Phytol. 93, 217–225.
Bååth E and Söderström B 1979 Fungal biomass and fungal immobilization of plant nutrients in Swedish coniferous forest soils. Rev. Ecol. Biol. Sol 16, 477–489.
Callow J A, Capaccio L C M, Parish G and Tinker P B 1978 Detection and estimation of polyphosphate in vesicular-arbuscular mycorrhizas. New Phytol. 80, 125–134.
Cooper K M and Tinker P B 1978 Translocation and transfer of nutrients in vesicular-arbuscular mycorrhizas. II. Uptake and translocation of phosphorus, zinc and sulphur. New Phytol. 81, 43–52.
Cox G, Moran K J, Sanders F E, Nockolds C and Tinker P B 1980 Translocation and transfer of nutrients in vesicular-arbuscular mycorrhizas. III. Polyphosphate granules and phosphorus translocation. New Phytol. 84, 649–659.
Gericke S and Kurmies B 1952 Die kolorimetrische Phosphorsäurebestimmung mit Ammonium-Vanadat-Molybdat und ihre Anwendung in der Pflanzenanalyse. Z. Pflanzenernaehr. Dueng. Bodenkd. 59, 235–247.
Gildon A and Tinker P B 1983 Interaction of vesicular-arbuscular mycorrhizal infections and heavy metals in plants. II. The effects of infection on uptake of copper. New Phytol. 95, 263–268.
Gnekow M A and Marschner H 1989 Role of VA-mycorrhiza in growth and mineral nutrition of apple (Malus pumila var.domestica) rootstock cuttings. Plant and Soil 119, 285–293.
Killham K and Firestone M K 1983 Vesicular-arbuscular mycorrhizal mediation of grass response to acidic and heavy metal depositions. Plant and Soil 72, 39–48.
Kormanik P P and McGraw A C 1984 Quantification of vesicular-arbuscular mycorrhizae in plant roots.In Methods and Principles of Mycorrhizal Research. Ed. N C Schenck. pp 37–45. The American Phytopathological Society, St. Paul, MN.
Kothari S K, Marschner H and Römheld V 1991 Contribution of the VA mycorrhizal hyphae in acquisition of phosphorus and zinc by maize grown in a calcareous soil. Plant and Soil 131, 177–185.
Lambert D H, Baker D E and Cole H 1979 The role of mycorrhizae in the interactions of phosphorus with zinc, copper, and other elements. Soil Sci. Soc. Am. J. 43, 976–980.
Li X-L, George E and Marschner H 1991 Extension of the phosphorus depletion zone in VA-mycorrhizal white clover in a calcareous soil. Plant and Soil 136, 41–48.
Loneragan J F 1982 Distribution and movement of copper in plants.In Copper in Soils and Plants. Eds. J F Loneragan, A D Robson and R D Graham. pp 165–188. Academic Press, Sydney.
Manjunath A and Habte M 1988 Development of vesicular-arbuscular mycorrhizal infection and the uptake of immobile nutrients inLeucaena leucocephala. Plant and Soil 106, 97–103.
Orlovich D A, Ashford A E and Cox G C 1989 A reassessment of polyphosphate granule composition in the ectomycorrhizal fungusPisolithus tinctorius. Aust. J. Plant Physiol. 16, 107–115.
Pacovsky R S 1986 Micronutrient uptake and distribution in mycorrhizal or phosphorus-fertilized soybeans. Plant and Soil 95, 379–388.
Rhodes L H and Gerdemann J W 1975 Phosphate uptake zones of mycorrhizal and non-mycorrhizal onions. New Phytol. 75, 555–561.
Rhodes L H and Gerdemann J W 1978 Translocation of calcium and phosphate by external hyphae of vesicular-arbuscular mycorrhizae. Soil Sci. 126, 125–126.
Sokal R R and Rohlf F J 1969 Biometry, 1st ed. W.H. Freeman, San Francisco, CA.
Swaminathan K and Verma B C 1979 Responses of three crop species to vesicular-arbuscular mycorrhizal infection on zinc-deficient Indian soils. New Phytol. 82, 481–487.
Timmer L W and Leyden R F 1980 The relationship of mycorrhizal infection to phosphorus-induced copper deficiency in sour organge seedlings. New Phytol. 85, 15–23.
Tinker P B and Gildon A 1983 Mycorrhizal fungi and ion uptake.In Metals and Micronutrients: Uptake and Utilization of Metals by Plants. Eds. D A Robb and W S Pierpoint. pp 21–32. Academic Press, London.
White J A and Brown M F 1979 Ultrastructure and X-ray analysis of phosphorus granules in a vesicular-arbuscular mycorrhizal fungus. Can. J. Bot. 57, 2812–2818.
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Li, XL., Marschner, H. & George, E. Acquisition of phosphorus and copper by VA-mycorrhizal hyphae and root-to-shoot transport in white clover. Plant Soil 136, 49–57 (1991). https://doi.org/10.1007/BF02465219
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DOI: https://doi.org/10.1007/BF02465219