Abstract
Key message
The seed reserves with 148 days after anthesis are sufficient to form 100% normal seedlings in vitro, obtaining a production gain of 101 days.
Abstract
Euterpe edulis (Arecaceae) is a typical palm of the Atlantic Forest with great ecological and economic value. Its seeds are characterized by hard coats, as well as recalcitrant, slow, and uneven germination, which hinder efficient seedling production. Given its risk of extinction, the objective of this study was to investigate reserve mobilization and how this impacted on the quality of maturing E. edulis seeds. Seven maturation stages separated by 20-day intervals, were assessed from 108 to 228 days after anthesis. The morphological characteristics, germination, vigor, and biochemical properties of in vitro germinated seeds, as well as the quality of the seedlings from each maturation stage were assessed. Four replications with ten seeds were evaluated at each stage. Over the experimental period, total relative growth rate of the fruits was 0.0037 mm day− 1, while moisture content decreased from 79.49 to 46.59%. The concentrations of phenols, soluble sugar, proteins, and starch decrease significantly during maturation, while there is an increase in lipids and fibers. Most variables displayed a positive correlation, except moisture, phenols, soluble sugar, proteins, and starch. E. edulis seeds possessed sufficient reserves to allow for normally developed embryos 148 days after anthesis, their 100% germination, and formation of 100% normal seedlings.
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References
Alabarce FS, Dillenburg LR (2017) Maternal light environment during seed development can affect seed quality of Euterpe edulis. Theor Exp Plant Physiol 29:1–11. https://doi.org/10.1007/s40626-016-0083-5
Alencar NL, Innecco R, Gomes-Filho E, Gallão MI, Alvarez-Pizarro JC, Prisco JT, Oliveira AB (2012) Seed reserve composition and mobilization during germination and early seedling establishment of Cereus jamacaru D.C. ssp. jamacaru (Cactaceae). An Acad Bras Ciênc 84:823–832. https://doi.org/10.1590/S0001-37652012000300024
Alqudah AM, Samarah NH, Mullen RE (2011) Drought stress effect on crop pollination, seed set, yield and quality. In: Lichtfouse E (ed) Alternative farming systems, biotechnology, drought stress and ecological fertilization. Springer, Dordrecht, pp 193–213
Andrade ACS (2001) The effect of moisture content and temperature on the longevity of heart of palm seeds (Euterpe edulis). Seed Sci Technol 29:171–182
Baskin CC, Baskin JM (2014) Seeds: ecology, biogeography, and evolution of dormancy and germination, 2nd edn. Elsevier, Amsterdam
Baskin JM, Baskin CC (2014b) What kind of seed dormancy might palms have? Seed Sci Res 24:17–22. https://doi.org/10.1017/S0960258513000342
Bligh EG, Dyer WJ (1959) A rapid method for total lipid extraction and purification. Can J Biochem Physiol 37:911–917. https://doi.org/10.1139/o59-099
Borges GSC, Vieira FGK, Copetti C, Gonzaga LV, Zambiazi RC, Mancini Filho J, Fett R (2011) Chemical characterization, bioactive compounds, and antioxidant capacity of jussara (Euterpe edulis) fruit from the Atlantic Forest in southern Brazil. Food Res Int 44:2128–2133. https://doi.org/10.1016/j.foodres.2010.12.006
Borges GDSC, Gonzaga LV, Jardini FA, Mancini Filho J, Heller M, Micke G, Costa ACO, Fett R (2013) Protective effect of Euterpe edulis M. on Vero cell culture and antioxidant evaluation based on phenolic composition using HPLC—ESI-MS/MS. Food Res Int 51:363–369. https://doi.org/10.1016/j.foodres.2012.12.035
Bovi MLA, Cardoso M (1975) Germinação de sementes de palmiteiro (Euterpe edulis Mart.). Bragantia 34:23–29. https://doi.org/10.1590/S0006-87051975000100028
Bradford MA (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Brasil (2008) Ministério do Meio Ambiente. Instrução Normativa n° 6, de 23 de setembro de 2008. Lista Oficial das Espécies da Flora Brasileira Ameaçadas de Extinção. Diário Oficial da União, Seção 01, pp 75–83. https://servicos.ibama.gov.br/phocadownload/legislacao/in_06_lista_flora_ameacada_extincao.pdf. Accessed 22 Sept 2021
Brasil (2009) Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Brasília: Mapa/ACS, p 399. https://www.gov.br/agricultura/pt-br/assuntos/insumos-agropecuarios/arquivos-publicacoes-insumos/2946_regras_analise__sementes.pdf. Accessed 22 Sept 2021
Brasil (2014) Ministério do Meio Ambiente. Portaria n.° 443, de 14 de dezembro de 2014. Lista Nacional Oficial de Espécies da Flora Ameaçadas de Extinção. Diário Oficial da União, Seção 01, p 121. http://cncflora.jbrj.gov.br/portal/static/pdf/portaria_mma_443_2014.pdf. Accessed 22 Sept 2021
Buckeridge MS, Tiné MA, Santos HP, Lima DU (2000) Polissacarídeos de reserva de parede celular em sementes, estrutura, metabolismo, funções e aspectos ecológicos. Rev Bras Fisiol Veg 12:137–162
Cabrera-Bosquet L, Sanchez C, Araus JL (2009) How yield relates to ash content, D13C and D18O in maize grown under different water regimes. Ann Bot 104:1207–1216. https://doi.org/10.1093/aob/mcp229
Carpiné D, Dagostin JLA, Mazon E, Barbi RCT, Alves FESB, Chaimsohn FP, Ribani RH (2019) Valorization of Euterpe edulis Mart. agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds. J Food Sci 85:96–104. https://doi.org/10.1111/1750-3841.14978
Chagas G, Salk CF, Vidal EJ, Souza SEXF, Brancalion PHS (2021) Exploiting fruits of a threatened palm to trigger restoration of Brazil’s Atlantic Forest. Restor Ecol. https://doi.org/10.1111/rec.13294
Cursi PR, Cicero SM (2014) Fruit processing and the physiological quality of Euterpe edulis Martius seeds. J Seed Sci 36:134–142. https://doi.org/10.1590/2317-1545v32n2847
Dickson A, Leaf AL, Hosner JF (1960) Quality appraisal of white spruce and white pine seedling stock in nurseries. For Chron 36:10–13. https://doi.org/10.5558/tfc36010-1
Donohue K (2009) Completing the cycle: material effects as the missing link in plant life histories. Philos Trans R Soc Lond B Biol Sci 364:1059–1074. https://doi.org/10.1098/rstb.2008.0291
Donohue K, Casas RR, Burghardt L, Kovach K, Willis CG (2010) Germination, post germination, adaptation, and species ecological ranges. Annu Rev Ecol Evol Syst 41:293–319. https://doi.org/10.1146/annurev-ecolsys-102209-144715
Dorneles LL, Zillikens A, Steiner J, Padilha MTS (2013) Biologia da polinização de Euterpe edulis Martius (Arecaceae) e associação com abelhas sociais (Apidae: Apini) em sistema agroflorestal na Ilha de Santa Catarina. Iheringia 68:47–57
Ferreira CD, Silva-Cardoso IMA, Ferreira JCB, Costa FHS, Scherwinski-Pereira JE (2020) Morphostructural and histochemical dynamics of Euterpe precatoria (Arecaceae) germination. J Plant Res 133:693–713. https://doi.org/10.1007/s10265-020-01219-7
Guerin C, Serret J, Montúfar R, Vaissayre V, Bastos-Siqueira A, Durand-Gasselin T, Tregear J, Morcillo F, Dussert S (2020) Palm seed and fruit lipid composition: phylogenetic and ecological perspectives. Ann Bot 125:157–172. https://doi.org/10.1093/aob/mcz175
Inada KOP, Oliveira AA, Revorêdo TB, Martins ABN, Lacerda ECQ, Freire AS, Braz BF, Santeli RE, Torres AG, Perrone D, Monteiro MC (2015) Screening of the chemical composition and occurring antioxidants in jabuticaba (Myrciaria jaboticaba) and jussara (Euterpe edulis) fruits and their fractions. J Funct Foods 17:422–433. https://doi.org/10.1016/j.jff.2015.06.002
Labouriau LG (1983) A germinação das sementes. Secretaria Geral da Organização dos Estados Americanos, Washington, p 174
Lai LM, Tian Y, Wang YJ, Zhao XC, Jiang LH, Baskin JM, Baskin CC, Zheng Y (2015) Distribution of three congeneric shrub species along an aridity gradient is related to seed germination and seedling emergence. AoB Plants 7:plv071. https://doi.org/10.1093/aobpla/plv071
Li R, Chen LJ, Wu YP, Zhang R, Baskin CC, Baskin JM, Hu X (2017) Effects of cultivar and maternal environment on seed quality in Vicia sativa. Front Plant Sci 8:1–9. https://doi.org/10.3389/fpls.2017.01411
Maguire JD (1962) Speeds of germination-aid selection and evaluation for seedling emergence and vigor. Crop Sci 2:176–177. https://doi.org/10.2135/cropsci1962.0011183X000200020033x
Mantovani A, Morellato P (2000) Fenologia da floração, frutificação, mudança foliar e aspectos da biologia floral. In: Reis A, Reis MS (eds) Euterpe edulis Martius: biologia, conservação e manejo sustentado. Herbário Barbosa Rodrigues, Itajaí, pp 23–38
Martins CC, Nakagawa J, Alves Bovi ML (2000) Desiccation tolerance of four seed lots from Euterpe edulis Mart. Seed Sci Technol 28:101–113
Mazzottini-dos-Santos HC, Ribeiro LM, Oliveira DMT (2017) Roles of the haustorium and endosperm during the development of seedlings of Acrocomia aculeata (Arecaceae): dynamics of reserve mobilization and accumulation. Protoplasma 254:1563–1578. https://doi.org/10.1007/s00709-016-1048-x
Montoya SG, Motoike SY, Kuki KN, Couto AD (2016) Fruit development, growth, and stored reserves in macauba palm (Acrocomia aculeata), an alternative bioenergy crop. Planta 244:927–938. https://doi.org/10.1007/s00425-016-2558-7
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nascimento JRS, Mélo Neto DF, Coutinho IC, Domont GB, Nogueira FCS, Campos FAP (2020) Proteome dynamics of the cotyledonary haustorium and endosperm in the course of germination of Euterpe oleracea seeds. Plant Sci 298:110569. https://doi.org/10.1016/j.plantsci.2020.110569
Nonogaki H, Bassel GW, Bewley JD (2010) Germination—still a mystery. Plant Sci 179:574–581. https://doi.org/10.1016/j.plantsci.2010.02.010
Panza V, Láinez V, Maldonado S (2004) Seed structure and histochemistry in the palm Euterpe edulis. Bot J Linn Soc 145:445–453. https://doi.org/10.1111/j.1095-8339.2004.00293.x
Pinol MT, Palazón J (1993) Fisiologia y bioquimica vegetal, 1st edn. McGraw Hill, Madrid, p 581
Queiroz MH (1986) Botão germinativo do palmiteiro como indicador de germinação. J Seed Sci 8:55–59
R Core Team (2020) A language and environment for statistical computing. Vienna: R Foundation for Statistical Computing, 2017. Disponível em: https://www.rproject.org/. Acesso em: 10 fev. 2020
Ribeiro LO, Pereira RNC, Tonon RV, Cabral LMC, Santiago MCPA, Vicente AA, Teixeira JAC, Matta VM, Freitas SP (2018) Antioxidant compounds recovery from juçara residue by thermal assisted extraction. Plant Foods Hum Nutr 73:68–73. https://doi.org/10.1007/s11130-017-0651-0
Schulz M, Borges GDSC, Gonzaga LV, Seraglio SKT, Olivo IS, Azevedo MS, Nehring P, Gois JS, Almeida TS, Vitali L, Spudeit DA, Micke GA, Borges DLG, Fett R (2015) Chemical composition, bioactive compounds and antioxidant capacity of juçara fruit (Euterpe edulis Martius) during ripening. Food Res Int 77:125–131. https://doi.org/10.1016/j.foodres.2015.08.006
Schulz M, Borges GDSC, Gonzaga LV, Costa ACO, Fett R (2016) Juçara fruit (Euterpe edulis Mart.): sustainable exploitation of a source of bioactive compounds. Food Res Int 89:14–26. https://doi.org/10.1016/j.foodres.2016.07.027
Schulz M, Biluca FC, Gonzaga LV, Borges GDSC, Vitali L, Micke GA, Gois JS, Almeida TS, Borges DL, Miller PR, Costa AC, Fett R (2017) Bioaccessibility of bioactive compounds and antioxidant potential of juçara fruits (Euterpe edulis Martius) subjected to in vitro gastrointestinal digestion. Food Chem 228:447–454. https://doi.org/10.1016/j.foodchem.2017.02.038
Schulz M, Brugnerotto P, Seraglio SKT, Gonzaga LV, Borges GSC, Costa ACO, Fett R (2021) Aliphatic organic acids and sugars in seven edible ripening stages of juçara fruit (Euterpe edulis Martius). J Food Compos Anal 95:103683. https://doi.org/10.1016/j.jfca.2020.103683
Schwember AR, Bradford KJ (2010) Quantitative trait loci associated withlongevity of lettuce seeds under conventional and controlled deterioration storage conditions. J Exp Bot 61:4423–4436. https://doi.org/10.1093/jxb/erq248
Silva JZ, Reis MS (2018) Fenologia reprodutiva e produção de frutos em Euterpe edulis (Martius). Cienc Florest 28:295–309. https://doi.org/10.5902/1980509831655
Soler-Guilhen JH, Bernardes CO, Marçal TS, Oliveira WBS, Ferreira MFS, Ferreira A (2020) Euterpe edulis seed germination parameters and genotype selection. Acta Sci Agron 42:e42461. https://doi.org/10.4025/actasciagron.v42i1.42461
Soriano D, Huante P, Gamboa-Debuen A, Orozco-Segovi A (2014) Effects of burial and storage on germination and seed reserves of 18 tree species in a tropical deciduous forest in Mexico. Oecologia 174:33–44. https://doi.org/10.1007/s00442-013-2753-1
Souza AC, Prevedello JA (2020) The importance of protected areas for overexploited plants: Evidence from a biodiversity hotspot. Biol Cons 243:108482. https://doi.org/10.1016/j.biocon.2020.108482
Swain T, Hills WE (1959) The phenolic constituents of Prunus domestica. I: quantitative analysis of phenolics constituents. J Sci Food Agric 19:63–68. https://doi.org/10.1002/jsfa.2740100110
Trevisan ACD, Fantini AC, Schmitt-Filho AL, Farley J (2015) Market for Amazonian açaí (Euterpe oleraceae) stimulates pulp production from Atlantic Forest juçara berries (Euterpe edulis). Agroecol Sustain Food Syst 39:762–781. https://doi.org/10.1080/21683565.2015.1025461
Yemm EW, Willis AJ (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57:508–514. https://doi.org/10.1042/bj0570508
Zhao M, Zhang H, Yan H, Qiu L, Baskin CC (2018) Mobilization and role of starch, protein, and fat reserves during seed germination of six wild grassland species. Front Plant Sci 9:1–11. https://doi.org/10.3389/fpls.2018.002347
Acknowledgements
We would like to thank the Fundação de Amparo à Pesquisa e Inovação do Espírito Santo (FAPES) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), for their financial support, and Editage (www.editage.com) for English language editing.
Funding
This study was funded in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—(Finance code 001), Fundação de Amparo à Pesquisa e Inovação do Espírito Santo (FAPES) (EDITAL FAPES/CNPq N° 05/2017—PRONEm—PROGRAMA DE APOIO A NÚCLEOS EMERGENTES. Agreement registered with SICONV under the n° 794009/2013 and the Process FAPES n° 72660945).
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de Mello, T., Rosa, T.L.M., Simões, I.M. et al. Reserve mobilization and in vitro germination of Euterpe edulis (Martius) seeds at different maturation stages. Trees 36, 415–426 (2022). https://doi.org/10.1007/s00468-021-02216-6
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DOI: https://doi.org/10.1007/s00468-021-02216-6