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Enhancing hybrid Liquidambar somatic seedling production using a temporary immersion bioreactor

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Temporary immersion bioreactors can improve hybrid sweetgum somatic embryo conversion and somatic seedling growth rates compared to semisolid medium, significantly improving somatic seedling production efficiency for hybrid sweetgum, an emerging woody biomass crop.

Abstract

Fast-growing hybrid southern hardwood trees should make excellent material for woody biomass production in the Southeastern US, if elite clones can be identified and efficiently propagated. We have enhanced the potential of sweetgum (Liquidambar styraciflua) as a biomass species by generating hybrids between the tree and its Chinese relative, Liquidambar formosana, and propagating the most promising clones via somatic embryogenesis. Some of the hybrid clones have already demonstrated superior biomass productivity compared to elite L. styraciflua trees. However, production of somatic seedlings from these clones remains labor-intensive. Bioreactors, specifically temporary immersion designs, such as the RITA®, have been applied to improve the efficiency of in vitro propagation of a number of woody species. We tested RITA® bioreactors for their potential to improve the production efficiency of high-quality hybrid sweetgum somatic seedlings. In one tested genotype, a RITA® with 50 somatic embryos had about 66% higher conversion frequency (p < 0.05) and produced about 40% more “high-quality” somatic seedlings (p < 0.05) than when somatic embryos were germinated on semisolid medium in GA-7 vessels. In all the genotypes we tested, somatic seedlings produced in RITA® bioreactors had higher survival percentages by the end of acclimatization than somatic seedlings produced on semisolid medium in GA-7 vessels.

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References

  • Albarrán J, Bertrand B, Lartaud M, Etienne H (2005) Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos. Plant Cell Tiss Org Cult 81:27–36

    Article  Google Scholar 

  • Cabasson C, Alvard D, Dambier D et al (1997) Improvement of Citrus somatic embryo development by temporary immersion. Plant Cell Tiss Org Cult 50:33–37. https://doi.org/10.1023/a:1005896725780

    Article  Google Scholar 

  • Chen C (2016) Cost analysis of plant micropropagation of Phalaenopsis. Plant Cell Tiss Org Cult 126:167–175

    Article  Google Scholar 

  • Dai J, Vendrame WA, Merkle SA (2004) Enhancing the productivity of hybrid yellow-poplar and hybrid sweetgum embryogenic cultures. In Vitro Cell Dev Biol Plant 40:376–383

    Article  Google Scholar 

  • Escalant J-V, Teisson C, Côte F (1994) Amplified somatic embryogenesis from male flowers of triploid banana and plantain cultivars (Musa spp.). In Vitro Cell Dev Biol Plant 30:181–186

    Article  Google Scholar 

  • Etienne H, Berthouly M (2002) Temporary immersion systems in plant micropropagation. Plant Cell Tiss Org Cult 69:215–231

    Article  Google Scholar 

  • Etienne H, Lartaud M, Michaux-Ferrière N et al (1997) Improvement of somatic embryogenesis in Hevea brasiliensis (Müll. Arg.) using the temporary immersion technique. In Vitro Cell Dev Biol Plant 33:81–87

    Article  Google Scholar 

  • Etienne-Barry D, Bertrand B, Vasquez N, Etienne H (1999) Direct sowing of Coffea arabica somatic embryos mass-produced in a bioreactor and regeneration of plants. Plant Cell Rep 19:111–117

    Article  CAS  Google Scholar 

  • Gao M, Jiang W, Wei S et al (2015) High-efficiency propagation of Chinese water chestnut [Eleocharis dulcis (Burm.f.) Trin. ex Hensch] using a temporary immersion bioreactor system. Plant Cell Tiss Org Cult 121:761–772

    Article  CAS  Google Scholar 

  • Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 107:161–170

    Article  CAS  Google Scholar 

  • Hlophe N, Moyo M, Staden J, Finnie J (2015) Micropropagation of Zantedeschia aethiopica (L.) spreng.: towards its commercial use in the cut flower industry. Propag Ornam Plants 15:73–78

    Google Scholar 

  • Holtz CT (2014) Enhancing chestnut embryogenic culture productivity and somatic embryo quality by targeting steps in the embryogenesis protocol. M.S. Thesis, University of Georgia, Athens, GA USA

  • Huxter TJ, Thorpe TA, Reid DM (1981) Shoot initiation in light-and dark-grown tobacco callus: the role of ethylene. Physiol Plant 53:319–326

    Article  CAS  Google Scholar 

  • Kitto SL (1997) Commercial micropropagation. HortScience 23:1012–1014

    Article  Google Scholar 

  • Kosky R, Perozo J, Valero N, Peñalver D (2005) Somatic embryo germination of Psidium guajava L. in the RITA® temporary immersion system and on semisolid medium. Liq Cult Syst In Vitro Plant Propag. https://doi.org/10.1007/1-4020-3200-5_14

    Article  Google Scholar 

  • Lal M, Tiwari A, Gupta G (2015) Commercial scale micropropagation of sugarcane: constraints and remedies. Sugar Tech 17:339–347

    Article  CAS  Google Scholar 

  • Loyola-Vargas VM, Avilez-Montalvo JR, Avilés-Montalvo RN et al (2016) Somatic embryogenesis in Coffea spp. In: Loyola-Vargas VM, Ochoa-Alejo N (eds) Somatic embryogenesis: fundamental aspects and applications. Springer International Publishing, Cham, pp 241–266

    Chapter  Google Scholar 

  • Ma J-H, Yao J-L, Cohen D, Morris B (1998) Ethylene inhibitors enhance in vitro root formation from apple shoot cultures. Plant Cell Rep 17:211–214

    Article  CAS  Google Scholar 

  • Mallón R, Covelo P, Vieitez AM (2012) Improving secondary embryogenesis in Quercus robur: application of temporary immersion for mass propagation. Trees 26:731–741. https://doi.org/10.1007/s00468-011-0639-6

    Article  Google Scholar 

  • Mamun N, Egertsdotter U, Aidun C (2015) Bioreactor technology for clonal propagation of plants and metabolite production. Front Biol 10:177–193

    Article  CAS  Google Scholar 

  • McAlister B, Finnie J, Watt MP, Blakeway F (2005) Use of the temporary immersion bioreactor system (RITA®) for production of commercial Eucalyptus clones in Mondi Forests (SA). Plant Cell Tissue Org Cult 81:347–358

    Article  Google Scholar 

  • Merkle S (2018) The ups and downs of developing hybrid sweetgum varieties for the US bioenergy and pulp and paper industries: a 20-year case study. In: Bonga JM, Park YS, Trontin JF (eds) Proceedings of the 5th international conference of the IUFRO Unit 20902 on “Clonal Trees in the Bioeconomy Age: Opportunities and Challenges”, Coimbra, pp 225–229

  • Merkle S, Cunningham M (2011) Southern hardwood varietal forestry: a new approach to short-rotation woody crops for biomass energy. J For 109:7–14

    Google Scholar 

  • Merkle SA, Cunningham M (2016) Application of hybrid breeding and somatic embryogenesis to develop sweetgum varieties for the bioenergy and pulp and paper industries. In: Adams J (ed) 33rd Southern forest tree improvement conference, Hot Springs, AR pp 55–57

  • Merkle SA, Montello PM, Kormanik T, Le H (2010) Propagation of novel hybrid sweetgum phenotypes for ornamental use via somatic embryogenesis. Propag Ornam Plants 10:220–226

    Google Scholar 

  • Michoux F, Ahmad N, Hennig A et al (2013) Production of leafy biomass using temporary immersion bioreactors: an alternative platform to express proteins in transplastomic plants with drastic phenotypes. Planta 237:903–908

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Nasri A, Baklouti E, Romdhane AB, Maalej M, Schumacher HM, Drira N, Fki L (2019) Large-scale propagation of Myrobolan (Prunus cerasifera) in RITA® bioreactors and ISSR-based assessment of genetic conformity. Sci Hort 245:144–153

    Article  CAS  Google Scholar 

  • Quiala E, Cañal MJ, Meijón M et al (2012) Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments. Plant Cell Tissue Org Cult 109:223–234

    Article  CAS  Google Scholar 

  • Sommer HE, Brown CL (1980) Embryogenesis in tissue cultures of sweetgum. For Sci 26(2):257–260

    Google Scholar 

  • Vendrame WA, Holliday CP, Merkle SA (2001) Clonal propagation of hybrid sweetgum (Liquidambar styraciflua × L. formosana) by somatic embryogenesis. Plant Cell Rep 20:691–695

    Article  CAS  Google Scholar 

  • Vidal N, Sanchez C (2019) Use of bioreactor systems in the propagation of forest trees. Eng Life Sci 19:896–915

    Article  CAS  Google Scholar 

  • Vidal N, Blanco B, Cuenca B (2015) A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut. Plant Cell Tissue Org Cult 123:229–243

    Article  CAS  Google Scholar 

  • Witham FH, Blaydes DF, Devlin RM (1971) Experiments in plant physiology. Van Nostrand Reinhold Co., New York

    Google Scholar 

  • Wright J, Cunningham M (2008) Sweetgum plantations for sawtimber, energy, pulp, and other uses. For Landowner 67(3):26–28

    Google Scholar 

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Acknowledgements

The research reported here was supported by ArborGen Inc. The authors would like to thank Paul Montello for his work on hybrid sweetgum embryogenic culture initiation and maintenance, and Christine Holtz and Lisheng Kong for instruction on RITA® operation.

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Authors and Affiliations

  1. Ministry of Education Key Laboratory for Earth System Modeling, Department of Earth System Science, Tsinghua University, Beijing, 100084, China

    Siran Lu

  2. Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA

    Siran Lu & Scott A. Merkle

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  1. Siran Lu
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  2. Scott A. Merkle
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Correspondence to Scott A. Merkle.

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Lu, S., Merkle, S.A. Enhancing hybrid Liquidambar somatic seedling production using a temporary immersion bioreactor. Trees 35, 503–512 (2021). https://doi.org/10.1007/s00468-020-02052-0

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