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Development of wheat bran hydrolysate as Komagataella phaffii medium for heterologous protein production

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Abstract

Developing a Komagataella phaffii (K. phaffii, named as Pichia pastoris formerly) medium using wheat bran hydrolysate (WBH) is a potential approach for wheat bran utilization and heterologous protein by K. phaffii because K. phaffii is used as protein producer by researchers and engineers widely. In this research, the detoxification process of WBH was optimized to obtain the final procedure as pH adjusting to 10 by calcium hydroxide addition, then, 2.0 g/L active carbon absorption followed by 1 h shaking and 3,600 × g centrifugation for 10 min, finally, 3.75 mmol/L sodium thiosulfate addition for 10 min shaking followed by 3,600 × g centrifugation for 10 min. Recombinant K. phaffii-xynB harboring xylanase B gene from Aspergillus niger ATCC 1015 under alcohol oxidase 1 promoter (PAOX1) was cultivated in detoxified WBH expressing 1059.8 U/mL xylanase B which was 90.9% of that in complex medium from Pichia protocols. These researches built a solid base for detoxified WBH as a low-cost medium of K. phaffii to express heterologous protein, also provided a bright outlet for wheat bran utilization.

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Acknowledgements

Project supported by the National Natural Science Foundation of China (No. 31870045).

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  1. Shanghai Engineering Research Center for Food Rapid Detection, Institute of Food Science and Engineering, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai, 200093, People’s Republic of China

    Ziwei Zhou, Hualan Zhou & Jianguo Zhang

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  1. Ziwei Zhou
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Correspondence to Jianguo Zhang.

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Zhou, Z., Zhou, H. & Zhang, J. Development of wheat bran hydrolysate as Komagataella phaffii medium for heterologous protein production. Bioprocess Biosyst Eng 44, 2645–2654 (2021). https://doi.org/10.1007/s00449-021-02633-4

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