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Cellulases immobilization on chitosan-coated magnetic nanoparticles: application for Agave Atrovirens lignocellulosic biomass hydrolysis

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Abstract

In the present study, Trichoderma reesei cellulase was covalently immobilized on chitosan-coated magnetic nanoparticles using glutaraldehyde as a coupling agent. The average diameter of magnetic nanoparticles before and after enzyme immobilization was about 8 and 10 nm, respectively. The immobilized enzyme retained about 37 % of its initial activity, and also showed better thermal and storage stability than free enzyme. Immobilized cellulase retained about 80 % of its activity after 15 cycles of carboxymethylcellulose hydrolysis and was easily separated with the application of an external magnetic field. However, in this reaction, K m was increased eight times. The immobilized enzyme was able to hydrolyze lignocellulosic material from Agave atrovirens leaves with yield close to the amount detected with free enzyme and it was re-used in vegetal material conversion up to four cycles with 50 % of activity decrease. This provides an opportunity to reduce the enzyme consumption during lignocellulosic material saccharification for bioethanol production.

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Acknowledgments

The work was financially supported by Grant (PDCPN2013-01) No. 213844 (CONACYT). We thank Mexican Council of Science and Technology (CONACYT) for Masters Scholarship and for the financial support under the program “Cátedras-CONACYT, 2015”, Grant No. 729.

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Authors and Affiliations

  1. Nanobioscience Group and Food Research Department, Chemistry Faculty, Universidad Autónoma de Coahuila, Blvd. V. Carranza e Ing. J. Cárdenas, 25280, Saltillo, Coahuila, Mexico

    Jaquelina Sánchez-Ramírez, José L. Martínez-Hernández, Patricia Segura-Ceniceros, Cristóbal N. Aguilar & Anna Ilyina

  2. Research Center of Applied Chemistry (CIQA), Blvd. Enrique Reyna Hermosillo 140, 25250, Saltillo, Coahuila, Mexico

    Guillermo López & Hened Saade

  3. Animal Nutrition Department, Universidad Autónoma Agraria Antonio Narro, Calzada Antonio Narro, Buenavista, 25000, Saltillo, Coahuila, Mexico

    Miguel A. Medina-Morales

  4. CONACYT-Universidad Autónoma de Coahuila, Blvd. V. Carranza e Ing. J. Cárdenas, 25280, Saltillo, Coahuila, Mexico

    Rodolfo Ramos-González

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  1. Jaquelina Sánchez-Ramírez
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  2. José L. Martínez-Hernández
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  3. Patricia Segura-Ceniceros
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  6. Miguel A. Medina-Morales
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  8. Cristóbal N. Aguilar
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  9. Anna Ilyina
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Correspondence to Anna Ilyina.

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Sánchez-Ramírez, J., Martínez-Hernández, J.L., Segura-Ceniceros, P. et al. Cellulases immobilization on chitosan-coated magnetic nanoparticles: application for Agave Atrovirens lignocellulosic biomass hydrolysis. Bioprocess Biosyst Eng 40, 9–22 (2017). https://doi.org/10.1007/s00449-016-1670-1

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