Abstract
Metathelazia capsulata (family Pneumospiruridae) is a lungworm parasitizing the bronchi and bronchioles, described in four species of wild carnivores. Very little molecular data are available on this nematode and none on other species of the Pneumospiruridae family. In this work, we describe for the first time the complete mitogenome (mitochondrial genome) of M. capsulata, being the first described of the family Pneumospiruridae. The mitogenome of M. capsulata has 13,659 bp in length, an A + T content of 79.2%. The mitogenome included 12 protein-coding genes (PCGs) (lacking the atp8 gene), 22 tRNA genes, 2 rRNA genes (all the genes are coded by the heavy strand), and an AT-rich region. The PCGs varied in size (232 bp-1645 bp). Only the tRNA-Trp has the standard cloverleaf secondary structure, while the other 21 do not. The AT-rich region, with a 90.5% A + T content and a length of 389 bp, is located between the cox3 and tRNA-Ala genes. Comparison with the mitogenomes of 29 species of Spiruromorpha infraorder, belonging to different families, demonstrates that M. capsulata mitogenome shared the common characteristics of most of them. The phylogeny constructions yielded phylogenies that were in agreement with the obtained previously by using sequences and gene order data of mitogenomes.
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Data availability
All relevant data and materials of the study are included in the manuscript and the supplementary data. The complete mitochondrial genome data of Metathelazia capsulata is available in GenBank database under the accession number OQ865340. Raw sequencing reads used in this study were deposited in the public repository BioProject with accession number PRJNA1030884 (http://www.ncbi.nlm.nih.gov/bioproject/1030884).
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Acknowledgements
This work was supported by the Autonomous Community of the Region of Murcia (Spain), through the Regional Programme for the Promotion of Research (Action Plan 2019) of the Seneca Foundation, Science and Technology Agency of the Region of Murcia [grant number 20952/PI/18]. This work was also supported by the Jaén University (Plan de Apoyo a la Investigación) and by the Andalusian Plan for Scientific Research (research group RNM-924).
Funding
This work was funded by the Autonomous Community of the Region of Murcia (Spain), by the Jaén University (Plan de Apoyo a la Investigación) and by the Andalusian Plan for Scientific Research (research group RNM-924).
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Conceived and designed the experiments (C.M. and A.S.); performed the sample collection and laboratory examination (C.M., I.A. and M.R.R.); performed the molecular analysis (G.A., E.E.M. and A.S.); wrote the manuscript (A.S.); reviewed and corrected the manuscript (C.M., I.A., M.R.R, G.A. and E.E.M.). All authors have read and agreed on the manuscript.
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Aleix-Mata, G., Arcenillas-Hernández, I., de Ybáñez, M.R.R. et al. Complete mitochondrial genome of Metathelazia capsulata (Pneumospiruridae) and comparison with other Spiruromorpha species. Parasitol Res 123, 3 (2024). https://doi.org/10.1007/s00436-023-08035-7
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DOI: https://doi.org/10.1007/s00436-023-08035-7