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Extracellular synthesis of silver nanoparticles using Bacillus megaterium against malarial and dengue vector (Diptera: Culicidae)

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Abstract

Biosynthesis of silver nanoparticles has provoked nowadays and alternative to physical and chemical approaches. In the present study, silver nanoparticles (AgNPs) were synthesized extracellular method using Bacillus megaterium. The AgNPs formations were confirmed initially through color change, and the aliquots were characterized through UV–visible spectrophotometer, followed by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) spectroscopy, and Fourier transform infrared (FTIR) spectra. The surface plasmon resonance band was shown at 430 nm in UV–vis spectrophotometer. The bioreduction was categorized through identifying the compounds responsible for the AgNP synthesis, and the functional group present in B. megaterium cell-free culture was scrutinized using FTIR. The topography and morphology of the particles were determined using SEM. In addition, this biosynthesized AgNPs were found to show higher insecticidal efficacy against vector mosquitoes. The LC50 and LC90 were found to be 0.567, 2.260; 0.90, 4.44; 1.349, 8.269; and 1.640, 9.152 and 0.240, 0.955; 0.331, 1.593; 0.494, 2.811; and 0.700, 4.435 with respect to the first, second, third, and fourth instar larvae of Culex quinquefasciatus and Aedes aegypti. All the calculated χ 2 values are highly significant compared with the tabulated value. Therefore, B. megaterium-synthesized silver nanoparticles would be used as a potent larvicidal agent against Cx. quinquefasciatus and Ae. aegypti.

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Acknowledgments

The authors gratefully acknowledge the management of M.K.M. Group of Colleges, Hodal and Thiagarajar College (Autonomous), Madurai, for providing the facilities to perform the research works in the PG and Research Department of Zoology and Microbiology. Author ANB thanked UGC-MANF, India, for the financial support, and CRME (ICMR), Madurai, kindly supplied eggs and larvae required during our work. We thank the Department of Chemistry, Madras University and Karunya University, Coimbatore, for the instrumental analysis.

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  1. Department of Zoology, M.K.M. Group of Colleges, Hodal, Haryana, India

    A. Najitha Banu

  2. Postgraduate and Research Department of Zoology, Thiagarajar College, Madurai, Tamil Nadu, India

    C. Balasubramanian

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Banu, A.N., Balasubramanian, C. Extracellular synthesis of silver nanoparticles using Bacillus megaterium against malarial and dengue vector (Diptera: Culicidae). Parasitol Res 114, 4069–4079 (2015). https://doi.org/10.1007/s00436-015-4635-4

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