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Distribution of transient receptor potential cation channel subfamily V member 1-expressing nerve fibers in mouse esophagus

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Abstract

Transient receptor potential cation channel subfamily V member 1 (TRPV1) plays a role in esophageal function. However, the distribution of TRPV1 nerve fibers in the esophagus is currently not well understood. In the present study, we investigated the distribution of TRPV1 and neurotransmitters released from TRPV1 nerve fibers in the mouse lower esophagus. Furthermore, we investigated changes in the presence of TRPV1 in the mouse model of esophagitis. Numerous TRPV1-immunoreactive nerve fibers were seen in both the submucosal layer and myenteric plexus of the lower esophagus and colocalized with calcitonin gene-related peptide (CGRP). TRPV1 colocalized with substance P in axons in the submucosal layer and myenteric plexus. TRPV1 colocalized with neuronal nitric oxide synthase in the myenteric plexus. We observed some colocalization of CGRP with the vesicular acetylcholine (ACh) transporter, packaging of ACh into synaptic vesicles after its synthesis in terminal cytoplasm, in the submucosal layer and myenteric plexus. In the esophagitis model, the number of the TRPV1 nerve fibers did not change, but their immunoreactive intensity increased compared with sham-operated mice. Inhibitory effect of exogenous capsaicin on electrically stimulated twitch contraction significantly increased in esophagitis model compared with the effect in sham-operated mice. Overall, these results suggest that TRPV1 nerve fibers projecting to both the submucosal and muscle layer of the esophagus are extrinsic spinal and vagal afferent neurons. Furthermore, TRPV1 nerve fibers contain CGRP, substance P, nitric oxide, and ACh. Therefore, acid influx-mediated TRPV1 activation may play a role in regulating esophageal relaxation.

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Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and by Taiho Pharmaceutical Co. Ltd, Tokyo, JAPAN.

Conflict of interest

Syunji Horie received a research grant for the present study from Taiho Pharmaceutical Co. Ltd. The other authors have no competing interests and have no conflict of interests.

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Author notes

  1. Takuji Hosoya

    Present address: Research Laboratories 2, Fuji Yakuhin Co., Ltd., Saitama, Japan

Authors and Affiliations

  1. Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Josai International University, 1 Gumyo, Togane, Chiba, 283-8555, Japan

    Kenjiro Matsumoto, Eriko Ishikawa, Kimihito Tashima & Syunji Horie

  2. Department of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8675, Japan

    Takuji Hosoya & Toshihiko Murayama

  3. Division of Pathological Sciences, Department of Pharmacology and Experimental Therapeutics, Kyoto Pharmaceutical University, Misasagi, Ymashina, Kyoto, 607-8414, Japan

    Kenjiro Matsumoto, Kikuko Amagase & Shinichi Kato

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  1. Kenjiro Matsumoto
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Correspondence to Kenjiro Matsumoto.

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Matsumoto, K., Hosoya, T., Ishikawa, E. et al. Distribution of transient receptor potential cation channel subfamily V member 1-expressing nerve fibers in mouse esophagus. Histochem Cell Biol 142, 635–644 (2014). https://doi.org/10.1007/s00418-014-1246-6

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