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Potential forensic use of a 33 X-InDel panel in the Argentinean population

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Abstract

Polymorphic genetic markers located on the X chromosome might become a complement in particular forensic identification when the biological kinship are deficient. We analyzed forensic statistical parameters of 33 X-chromosome InDel polymorphisms in a sample of 320 individuals from Argentina. The X-chromosome InDel polymorphism (X-InDel) panel was amplified in a single multiplex PCR reaction. Hardy-Weinberg Equilibrium was determined in the female cohort, whereas the male cohort was used to calculate linkage disequilibrium (LD) tested by an extension of Fisher’s exact test, D’, and Chi-square values. Regarding LD, 15 markers were organized and grouped into six blocks containing two or three linked loci each, namely block I (MID356-MID357), block II (MID448804-MID3703-MID218), block III (MID3705-MID3706-MID304737), block IV (MID197147-MID3754), block V (MID3664-MID284601-MID103547), and block VI (MID3763-MID3728). The haplotype diversity was higher than 0.99 in all cases. Blocks III and VI showed the highest match probability in the studied population, whereas block II showed the lowest. The accumulated power of discrimination was 99.9999991 % in women and 99.9992925 % in men. The mean exclusion chance in trios and duos were 99.9891736 and 99.6099391 %, respectively. Since 15 markers are associated as haplotypic blocks, for a conservative treatment of the data, statistical evaluation should consider their haplotypic frequencies and the remaining 18 markers can be evaluated as independent loci.

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Acknowledgments

Daniel Corach and Mariela Caputo are members of Carrera del Investigador Cientifico CONICET (National Scientific and Technical Research Council). This work was supported, in part, by grants PIP 112-200801-02836 (CONICET) and 20020100100744 (UBACyT) to Daniel Corach. The funding agencies had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Department of Forensic Genetics and DNA Fingerprinting Service, School of Pharmacy and Biochemistry, Universidad de Buenos Aires, Junin 956, 7mo piso, C1113AAD, CABA, Buenos Aires, Argentina

    Mariela Caputo & D. Corach

  2. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina

    Mariela Caputo & D. Corach

  3. Laboratorio de Genética Humana e Médica, Departamento de Patologia, Universidade Federal do Pará, Belém, Pará, Brazil

    M. A. Amador & S. Santos

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  1. Mariela Caputo
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  2. M. A. Amador
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  3. S. Santos
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  4. D. Corach
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Correspondence to Mariela Caputo.

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Figure S1

InDel markers localization on the X-chromosome. Localization data are based on Freitas et al. 2010. Linkage blocks are indicated in blue. (GIF 26 kb)

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Table S1

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Caputo, M., Amador, M.A., Santos, S. et al. Potential forensic use of a 33 X-InDel panel in the Argentinean population. Int J Legal Med 131, 107–112 (2017). https://doi.org/10.1007/s00414-016-1399-z

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