Abstract
Purpose
There is controversy about the effect of the rs1799983 nitric oxide synthase (NOS3) genetic variant on hypertension and blood pressure (BP) levels. The aims of the current study were to examine whether rs1799983 affects BP levels and to identify potential interactions between this polymorphism and other non-genetic risk factors.
Methods
A total of 705 subjects were examined for anthropometric and body composition measurements, BP, dietary habits and physical activity. Oral epithelial cells were collected for the identification of rs1799983 using Luminex® 100/200TM System.
Results
After adjusted for covariates, TT genotype showed a 2.30-fold higher predisposition of hypertension than GG genotype subjects. According to BP levels, for each risk allele diastolic blood pressure (DBP) increased in 1.99 mmHg. Significant interactions between rs1799983 and saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) were found. Moreover, an interaction with body weight status was observed. Among overweight individuals, T allele carriers showed higher DBP than GG genotype.
Conclusion
The present study evidenced that rs1799983 NOS3 polymorphism could be associated with hypertension and DBP among Southern Europeans, being this association influenced by dietary fat (SFA and MUFA) and body mass index.
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Acknowledgments
We thank all the seekers of the Nutrigenetic Service who voluntarily offered their data. The authors are grateful to the five nutritionists for data collection and to Amaia Ibañez for excellent technical assistance. The authors thank the Linea Especial (University of Navarra; LE/97) and to the Spanish Ministry of Economy and Competitiveness (AGL2013-45554-R project) for financial support, CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn)/RETICS schedules (Instituto de Salud Carlos III, Madrid, Spain) for assistance in this study and the support from CINFA (Olloki, Spain) concerning the genetic tools and general logistic. The predoctoral research grant to Leticia Goñi from the Asociación de Amigos Universidad de Navarra is gratefully acknowledged.
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The Research Ethics Committee of the University of Navarra provided confirmation of fulfillment of the ethical standards affecting this research (Ref. 2410/2014). Therefore, the survey was in accordance with the principles of the 1964 Declaration of Helsinki and its later amendments.
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Goni, L., Cuervo, M., Milagro, F.I. et al. Influence of fat intake and BMI on the association of rs1799983 NOS3 polymorphism with blood pressure levels in an Iberian population. Eur J Nutr 56, 1589–1596 (2017). https://doi.org/10.1007/s00394-016-1203-3
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DOI: https://doi.org/10.1007/s00394-016-1203-3