Abstract
An experiment of pakchoi after conventional fertilization was conducted with Chlorella emersonii inoculation. The yield, nitrogen use efficiency, and rhizosphere microbial community of pakchoi were investigated, and the relationship among them was also determined. The results showed that C. emersonii inoculation with half dose increased the fresh weight and nitrogen use efficiency of pakchoi by 6.5% and 28.1%, respectively. Rhizosphere microbial community structure after C. emersonii inoculation was distinctly affected, which reflected the higher bacterial alpha diversity and lower fungal alpha diversity, and had positive and negative correlations with pakchoi performance characteristics, respectively. The dominant phyla showed that C. emersonii inoculation significantly increased the abundance of Proteobacteria and decreased the abundance of Actinobacteria for bacteria, however, all fungal abundances also decreased. At a higher classification resolution, C. emersonii inoculation enriched the indigenous Sphingomonas and introduced the nonindigenous Pseudofulvimonas involved in the nitrogen cycle, which might be responsible for the improvement of pakchoi yield by enhancing nitrogen use efficiency. The higher bacterial alpha diversity with enriched Sphingomonas and Pseudofulvimonas might provide a driving force for the greater pakchoi yield and nitrogen use efficiency under the half dose of C. emersonii inoculation compared with the total dose. This study suggested that C. emersonii inoculation improved the yield and nitrogen use efficiency of pakchoi through the amendment of beneficial rhizosphere microflora, especially those related to the nitrogen cycle, and confirmed the positive effectiveness of conventional fertilization regimes together with C. emersonii inoculation on fertilization efficiency and agricultural production.
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Funding
This study was supported by the National Key Research and Development Program of China (Grant No. 2021YFD1901100), State Key Laboratory of Integrative Sustainable Dryland Agriculture (in preparation), Shanxi Agricultural University (Grant No. 202105D121008-3-6), Six New Project of Agriculture Department of Shanxi Province (unnumbered), National Natural Science Foundation of China (Grant No. 31902394), Key Research and Development Planning Project of Shanxi Province (Grant No. 201803D31063), Science and Technology Innovation Planning Project of Shanxi Agricultural University (Grant No. 2018YJ16), Outstanding Doctor to Work in Shanxi Province Research Project (Grant No. SXYBKY2019036), Scientific and Technologial Innovation Programs of Higher Education Institutions in Shanxi (Grant No. 2021L119).
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Chen Ma: Conceptualization, Data curation, Formal analysis, Investigation, Visualization, Writing; Jingjie Zhang: Conceptualization; Zhengyi Liu & Song Qin: Methodology; Runzhi Li: Data curation; Hongli Cui: Conceptualization, Methodology, Data curation.
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Ma, C., Zhang, J., Liu, Z. et al. Chlorella emersonii Inoculation Improves Pakchoi Yield and Nitrogen Use Efficiency Through Amending the Rhizosphere Microbial Community. J Plant Growth Regul (2024). https://doi.org/10.1007/s00344-024-11327-6
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DOI: https://doi.org/10.1007/s00344-024-11327-6