Abstract
Key message
This review contains functional roles of MYB transcription factors in the transcriptional regulation of anthocyanin biosynthesis in horticultural plants. This review describes potential uses of MYB TFs as tools for metabolic engineering for anthocyanin production.
Abstract
Anthocyanins (ranging from red to blue) are controlled by specific branches of the anthocyanin biosynthetic pathway and are mostly visible in ornamentals, fruits, and vegetables. In the present review, we describe which R2R3-MYB transcription factors (TFs) control the transcriptional regulation of anthocyanin structural genes involved in the specific branches of the anthocyanin biosynthetic pathway in various horticultural plants (e.g., ornamentals, fruits, and vegetables). In addition, some MYBs responsible for anthocyanin accumulation in specific tissues are described. Moreover, we highlight the phylogenetic relationships of the MYBs that suppress or promote anthocyanin synthesis in horticultural crops. Enhancement of anthocyanin synthesis via metabolic genetic engineering of anthocyanin MYBs, which is described in the review, is indicative of the potential use of the mentioned anthocyanin-related MYBs as tools for anthocyanin production. Therefore, the MYBs would be suitable for metabolic genetic engineering for improvement of flower colors, fruit quality, and vegetable nutrients.
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References
Aharoni A, Ric De CH, Wein M, Sun ZK, Greco R, Kroon A, Mol JNM, O’Connell AP (2001) The strawberry FaMYB1 transcription factor suppresses anthocyanin and Xavonol accumulation in transgenic tobacco. Plant J 28:319–332
Ai TN, Naing AH, Arun M, Lim SH, Kim CK (2016) Sucrose-induced anthocyanin accumulation in vegetative tissue of petunia plants requires anthocyanin regulatory transcription factors. Plant Sci 252:144–150
Ai TN, Naing AH, Arun M, Jeon SM, Kim CK (2017) Expression of RsMYB1 in petunia enhances anthocyanin production in vegetative and floral tissues. Sci Hortic 214:58–65
Akagi T, Ikegami A, Yonemori K (2010) DkMyb2 wound-induced transcription factor of persimmon (Diospyros kaki Thunb.) contributes to proanthocyanidin regulation. Planta 232:1045–1059
Albert NW, Lewis DH, Zhang H, Irving LJ, Jameson PE, Davies KM (2009) Light-induced vegetative anthocyanin pigmentation in petunia. J Exp Bot 60:2191–2202
Albert NW, Arathoon S, Collette VE, Schwinn KE, Jameson PE, Lewis DH (2010) Activation of anthocyanin synthesis in Cymbidium orchids: variability between known regulators. Plant Cell Tiss Organ Cult 100:355–360
Albert NW, Lewis DH, Zhang H, Schwinn KE, Jameson PE, Davies KM (2011) Members of an R2R3-MYB transcription factor family in petunia are developmentally and environmentally regulated to control complex floral and vegetative pigmentation patterning. Plant J 65:771–784
Albert NW, Davies KM, Lewis DH, Zhang H, Montefiori M, Brendolise C (2014) A conserved network of transcriptional activators and repressors regulates anthocyanin pigmentation in eudicots. Plant Cell 26:962–980
Allan AC, Hellens RP, Laing WA (2008) MYB transcription factors that colour our fruit. Trends Plant Sci 13:99–102
An XH, Tian Y, Chen KQ, Liu XJ, Liu DD, Xie XB (2015) MdMYB9 and MdMYB11 are involved in the regulation of the JA-induced biosynthesis of anthocyanin and proanthocyanidin in apples. Plant Cell Physiol 56:650–662
Bagchi D, Sen CK, Ray SD, Das DK, Bagchi M, Preuss HG (2003) Molecular mechanisms of cardioprotection by a novel grape seed proanthocyanidin extract. Mutat Res 523–524:87–97
Ban Y, Honda C, Hatsuyama Y, Igarashi M, Bessho H, Moriguchi T (2007) Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin. Plant Cell Physiol 48:958–970
Ben ZMM, Negre-Zakharov F, Masci T, Ovadis M, Shklarman E (2008) Interlinking showy traits: co-engineering of scent and colour biosynthesis in flowers. Plant Biotechnol 6:403–415
Borevitz JO, Xia YJ, Blount J, Dixon RA, Lamb C (2000) Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell 12:2383–2393
Borovsky Y, Oren-Shamir M, Ovadia R, Jong WD, Paran I (2004) The A locus that controls anthocyanin accumulation in pepper encodes a MYB transcription factor homologous to anthocyanin2 of petunia. Theor Appl Genet 109:23–29
Butelli E, Titta L, Giorgio M, Mock HP, Matros A, Peterek S (2008) Enrichment of tomato fruit with health promoting anthocyanins by expression of select transcription factors. Nat Biotech 26:1301–1308
Butelli E, Licciardello C, Zhang Y, Liu J, Mackay S, Bailey P (2012) Retrotransposons control fruit-specific, cold-dependent accumulation of anthocyanins in blood oranges. Plant Cell 24:1242–1255
Cavallini E, Zenoni S, Finezzo L, Guzzo F, Zamboni A, Avesani L (2014) Functional diversification of grapevine MYB5a and MYB5b in the control of flavonoid biosynthesis in a petunia anthocyanin regulatory mutant. Plant Cell Physiol 55:517–534
Cavallini E, Matus JT, Finezzo L, Zenoni S, Loyola R, Guzzo F (2015) The phenylpropanoid pathway is controlled at different branches by a set of R2R3-MYB C2 repressors in grapevine. Plant Physiol 167:1448–1470
Chagné D, Lin-Wang K, Espley RV, Volz RK, How NM, Rouse S (2013) An ancient duplication of apple MYB transcription factors is responsible for novel red fruit-flesh phenotypes. Plant Physiol 161:225–239
Chiou CY, Yeh KW (2008) Differential expression of MYB gene (OgMYB1) determines color patterning in floral tissue of Oncidium Gower Ramsey. Plant Mol Biol 66:379–388
Chiu LW, Li L (2012) Characterization of the regulatory network of BoMYB2 in controlling anthocyanin biosynthesis in purple cauliflower. Planta 236:1153–1164
Chiu LW, Zhou X, Burke S, Wu X, Prior RL, Li L (2010) The purple cauliflower arises from activation of a MYB transcription factor. Plant Physiol 154:1470–1480
Cui B, Hu Z, Zhang Y, Hu J, Yin W, Feng Y (2016) Anthocyanins and flavonols are responsible for purple color of Lablab purpureus (L.) sweet pods. Plant Physio Biochem 103:183–190
Davies KM, Albert NW, Schwinn KE (2012) From landing lights to mimicry: the molecular regulation of flower colouration and mechanisms for pigmentation patterning. Funct Plant Biol 39:619–638
Deluc L, Barrieu F, Marchive C, Lauvergeat V, Decendit A, Richard T (2006) Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway. Plant Physiol 140:499–511
Elomaa P, Uimari A, Mehto M, Albert VA, Laitinen RAE, Teeri TH (2003) Activation of anthocyanin biosynthesis in Gerbera hybrida (Asteraceae) suggests conserved protein-protein and protein-promoter interactions between the anciently diverged monocots and eudicots. Plant Physiol 133:1831–1842
Espley RV, Hellens RP, Putterill J, Stevenson DE, Kutty-Amma S, Allan AC (2007) Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10. Plant J 49:414–427
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Feng S, Wang Y, Yang S, Xu Y, Chen X (2010) Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10. Planta 232:245–255
Feng K, Xu ZS, Que F, Liu JX, Wang F, Xiong AS (2018) An R2R3MYB transcription factor, OjMYB1, functions in anthocyanin biosynthesis in Oenanthe javanica. Planta 247:301–315
Fraser LG, Seal AG, Montefiori M, McGhie TK, Tsang GK, Datson PM (2013) An R2R3 MYB transcription factor determines red petal colour in an Actinidia (kiwifruit) hybrid population. BMC Genom 14:28
Griesbach RJ, Klein TM (1993) In situ genetic complementation of a flower color mutant in Doritis pulherrima (Orchidaceae). Lindleyana 8:223–226
He Q, Zhang ZF, Zhang LG (2016) Anthocyanin accumulation, antioxidant ability and stability, and a transcriptional analysis of anthocyanin biosynthesis in purple heading Chinese cabbage (Brassica rapa L. ssp. Pekinensis). J Food Chem 64:132–145
Hichri I, Barrieu F, Bogs J, Kappel C, Delrot S, Lauvergeat V (2011) Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway. J Exp Bot 62:2465–2483
Hou DX (2003) Potential mechanisms of cancer chemoprevention by anthocyanins. Curr Mol Med 3:149–159
Hsu CC, Chen YY, Tsai WC, Chen WH, Chen HH (2015) Three R2R3-MYB transcription factors regulate distinct floral pigmentation patterning in Phalaenopsis orchids. Plant Physiol 133:1831–1842
Huang W, Sun W, Lv H, Luo M, Zeng S, Pattanaik S (2013) A R2R3-MYB transcription factor from Epimedium sagittatum regulates the flavonoid biosynthetic pathway. PLoS ONE 8:e70778
Huang YF, Vialet S, Guiraud JL, Torregrosa L, Bertrand Y, Cheynier V (2014) A negative MYB regulator of proanthocyanidin accumulation, identified through expression quantitative locus mapping in the grape berry. New Phytol 201:795–809
Huang W, Khaldun ABM, Lv H, Du L, Zhang C, Wang Y (2016) Isolation and functional characterization of a R2R3-MYB regulator of the anthocyanin biosynthetic pathway from Epimedium sagittatum. Plant Cell Rep 35:883–894
Jia X, Shen J, Liu H, Li F, Ding N, Gao C (2015) Small tandem target mimic-mediated blockage of microRNA858 induces anthocyanin accumulation in tomato. Planta 242:283–293
Jin W, Wang H, Li M, Wang J, Yang Y, Zhang X (2016) The R2R3 MYB transcription factor PavMYB10. 1 involves in anthocyanin biosynthesis and determines fruit skin colour in sweet cherry (Prunus avium L.). Plant Biotechnol J 14:2120–2133
Jung CS, Griffiths HM, De Jong DM, Cheng S, Bodis M (2009) The potato developer (D) locus encodes an R2R3 MYB transcription factor that regulates expression of multiple anthocyanin structural genes in tuber skin. Theor Appl Genet 120:45–57
Kadomura-Ishikawa Y, Miyawaki K, Takahashi A (2015) RNAi-mediated silencing and overexpression of the FaMYB1 gene and its effect on anthocyanin accumulation in strawberry fruit. Biol Plantarum 59:677–685
Kee ES, Naing AH, Lim SH, Han JS, Kim CK (2016) MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars. 3 Biotech 6:79
Kiferle C, Fantini E, Bassolino L, Povero G, Spelt C, Buti S et al (2015) Tomato R2R3-MYB proteins SlANT1 and SlAN2: same protein activity, different roles. PLoS ONE 10:e0136365
Klatsky AL (2002) Alcohol and cardiovascular diseases: a historical overview. Ann N Y Acad Sci 957:7–15
Kobayashi S, Ishimaru M, Hiraoka K, Honda C (2002) Mybrelated genes of the Kyoho (Vitis labruscana) regulate anthocyanin biosynthesis. Planta 215:924–933
Kortstee AJ, Khan SA, Helderman C, Trindade LM, Wu Y, Visser RG (2011) Anthocyanin production as a potential visual selection marker during plant transformation. Transgenic Res 20:1253–1264
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874
Lai B, Li XJ, Hu B, Qin YH, Huang XM, Wang HC (2014) LcMYB1 is a key determinant of differential anthocyanin accumulation among genotypes, tissues, developmental phases and ABA and light stimuli in Litchi chinensis. PLoS ONE 9:e86293
Laitinen RAE, Ainasoja M, Broholm SK, Teeri TH, Elomaa P (2008) Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida. J Exp Bot 59:3691–3703
Li JG, Li HL, Peng SQ (2011) Three r2r3-myb transcription factor genes from capsicum annuum showing differential expression during fruit ripening. Afr J Biotechnol 10:8267–8274
Li L, Ban Z, Li X, Wu M, Wang A, Jiang Y (2012) Differential expression of anthocyanin biosynthetic genes and transcription factor PcMYB10 in pears (Pyruscommunis L.). PLoS ONE 7:e46070
Li C, Qui J, Yang G, Haung S, Yin J (2016) Isolation and characterization of a R2R3-MYB transcription factor gene related to anthocyanin biosynthesis in the spathes of Anthurium andraeanum (Hort.). Plant Cell Rep 35:2151–2165
Li C, Qui J, Ding L, Huang M, Huang S, Yang G (2017) Anthocyanin biosynthesis regulation of DhMYB2 and DhbHLH1 in Dendrobium hybrids petals. Plant Physiol Biochem 112:335–345
Lila MA (2004) Anthocyanins and human health: an in vitro investigative approach. J Biomed Biotechnol 5:306–313
Lim SH, Song JH, Kim DH, Kim JK, Lee JY, Kim YM (2016) Activation of anthocyanin biosynthesis by expression of the radish R2R3-MYB transcription factor gene RsMYB1. Plant Cell Rep 35:641–653
Lin-Wang K, Bolitho K, Grafton K, Kortstee A, Karunairetnam S, McGhie TK (2010) An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae. BMC Plant Biol 10:50
Liu X, Xiang L, Yin X, Grierson D, Li F, Chen K (2015) The identification of a MYB transcription factor controlling anthocyanin biosynthesis regulation in chrysanthemum flowers. Sci Hortic 194:278–285
Liu Y, Lin-Wang K, Espley RV, Wang L, Yang H, Yu B (2016) Functional diversification of the potato R2R3 MYB anthocyanin activators AN1, MYBA1, and MYB113 and their interaction with basic helix-loop-helix cofactors. J Exp Bot 67:2159–2176
Lowry DB, Sheng CC, Lasky JR, Willis JH (2012) Five anthocyanin polymorphisms are associated with an R2R3-MYB cluster in Mimulus guttatus (Phrymaceae). Am J Bot 99:82–91
Ma H, Pooler M, Griesbach R (2008) Ratio of Myc and Myb transcription factors regulates anthocaynin production in orchid flowers. J Am Soc Hortic Sci 133:133–138
Martin C, Prescott A, Mackay S, Bartlett J, Vrljlandt E (1991) Control of anthocyanin biosynthesis in flowers of Antirrbinum majus. Plant J 1:37–49
Mathews H, Clendennen SK, Caldwell CG, Liu XL, Connors K, Matheis N (2003) Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis, modification, and transport. Plant Cell 15:1689–1703
Medina-Puche L, Cumplido-Laso G, Amil-Ruiz F, Hoffmann T, Ring L, Rodríguez-Franco (2014) MYB10 plays a major role in the regulation of flavonoid/phenylpropanoid metabolism during ripening of Fragaria × ananassa fruits. J Exp Bot 65:401–417
Meng X, Wang JR, Wang GD, Liang XQ, Li XD, Meng QW (2015) An R2R3-MYB gene, LeAN2, positively regulated the thermo-tolerance in transgenic tomato. J Plant Physiol 175:1–8
Mol J, Grotewold E, Koes R (1998) How genes paint flowers and seeds. Trends Plant Sci 3:212–217
Morita Y, Saitoh M, Hoshino A, Nitasaka E, Iida S (2006) Isolation of cDNAs for R2R3-MYB, bHLH and WDR transcriptional regulators and Identification of c and ca mutations conferring white flowers in the Japanese morning glory. Plant Cell Physiol 47:457–470
Moyano E, Martinez-Garcia JF, Martin C (1996) Apparent redundancy in myb gene function provides gearing for the control of flavonoid biosynthesis in Antirrhinum flowers. Plant Cell 8:1519–1532
Naing AH, Ai TN, Jeon SM, Park KI, Lim KM, Kim CK (2015) Expression of RsMYB1 in chrysanthemum regulates key anthocyanin biosynthetic genes. Elect J of Biotechnol 18:359–364
Nakatsuka T, Haruta KS, Pitaksutheepong C, Abe Y, Kakizaki Y, Yamamoto K (2008) Identification and characterization of R2R3-MYB and bHLH transcription factors regulating anthocyanin biosynthesis in gentian flowers. Plant Cell Physiol 49:1818–1829
Nakatsuka A, Yamagishi M, Nakano M, Tasaki K, Kobayashi N (2009) Light-induced expression of basic helix–loop–helix genes involved in anthocyanin biosynthesis in flowers and leaves of Asiatic hybrid lily. Sci Hortic 121:84–91
Nishijima T, Morita Y, Sasaki K, Nakayama M, Yamaguchi H, Ohtsubo N (2013) A torenia (Torenia fournieri Lind. ex Fourn.) novel mutant ‘flecked’ produces variegated flowers by insertion of a DNA transposon into an R2R3-MYB gene. J Japanese Soc Horticult Sci 82:39–50
Niu SS, Xu CJ, Zhang WS, Zhang B, Li X, Lin-Wang K (2009) Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor. Planta 6:101–112
Palapol Y, Ketsa S, Lin-Wang K, Ferguson IB, Allan AC (2009) A MYB transcription factor regulates anthocyanin biosynthesis in mangosteen (Garcinia mangostana L.) fruit during ripening. Planta 229:1323–1334
Park NI, Xu H, Li X, Jang IH, Park S, Ahn GH (2011) Anthocyanin accumulation and expression of anthocyanin biosynthetic genes in radish (Raphanus sativus). J Agri Food Chem 59:6034–6039
Pérez-Díaz JR, Pérez-Díaz J, Madrid-Espinoza J, González-Villanueva E, Moreno Y, Ruiz-Lara S (2015) New member of the R2R3-MYB transcription factors family in grapevine suppresses the anthocyanin accumulation in the flowers of transgenic tobacco. Plant Mol Biol 90:63–76
Pervaiz S (2003) Resveratrol: from grapevines to mammalian biology. FASEB J 17:1975–1985
Pierantoni L, Dondini L, Franceschi PD, Musacchi S, Winkel BSJ, Sansavini S (2010) Mapping of an anthocyanin-regulating MYB transcription factor and its expression in red and green pear, Pyrus communis. Plant Physiol Bioch 48:1020–1026
Quattrocchio F, Wing JF, Leppen HTC, Mol JNM, Koes RE (1993) Regulatory genes controlling anthocyanin pigmentation are functionally conserved among plant species and have distinct sets of target genes. Plant Cell 5:1497–1512
Quattrocchio F, Wing JF, van der Woude K, Mol JNM, Koes R (1998) Analysis of bHLH and MYB–domain proteins: Species–specific regulatory differences are caused by divergent evolution of target anthocyanin genes. Plant J 13:475–488
Rahim MA, Busatto N, Trainotti L (2014) Regulation of anthocyanin biosynthesis in peach fruits. Planta 240:913–929
Ramsay NA, Glover BJ (2005) MYB-bHLH-WD40 protein complex and the evolution of cellular diversity. Trends Plant Sci 10:63–70
Ravaglia D, Espley RV, Henry-Kirk RA, Andreotti C, Ziosi V, Hellens RP (2013) Transcriptional regulation of flavonoid biosynthesis in nectarine (Prunus persica) by a set of R2R3 MYB transcription factors. BMC Plant Biol 13:68
Salvatierra A, Pimentel P, Moya-León MA, Herrera R (2013) Increased accumulation of anthocyanins in Fragaria chiloensis fruits by transient suppression of FcMYB1 gene. Phytochemistry 90:25–36
Schwinn K, Venail J, Shang Y, Mackay S, Alm V, Butelli E (2006) A small family of MYB-regulatory genes controls floral pigmentation intensity and patterning in the genus Antirrhinum. Plant Cell 18:831–851
Schwinn KE, Boase MR, Bradley JM, Lewis DH, Deroles SC, Martin CR (2014) MYB and bHLH transcription factor transgenes increase anthocyanin pigmentation in petunia and lisianthus plants, and the petunia phenotypes are strongly enhanced under field conditions. Front Plant Sci 5:603
Schwinn KE, Ngo H, Kenel F, Brummell DA, Albert NW, McCallum JA (2016) The onion (Allium cepa L.) R2R3-MYB gene MYB1 regulates anthocyanin biosynthesis. Front Plant Sci 7:1865
Shang Y, Venail J, Mackay S, Bailey PC, Schwinn KE, Jameson PE (2011) The molecular basis for venation patterning of pigmentation and its effect on pollinator attraction in flowers of Antirrhinum. New Phytol 189:602–615
Shen X, Zhao K, Liu L, Zhang K, Yuan H, Liao X (2014) A role for PacMYBA in ABA-regulated anthocyanin biosynthesis in red-colored sweet cherry cv. Hong Deng (Prunus avium L.). Plant Cell Physio 55:862–880
Sooriyapathirana S, Khan A, Sebolt A, Wang D, Bushakra J, Lin-Wang K (2010) QTL analysis and candidate gene mapping for skin and flesh colour in sweet cherry fruit (Prunus avium L.). Tree Genet Genomes 6:821–832
Spelt C, Quattrocchio F, Mol JNM, Koes R (2000) Anthocyanin1 of petunia encodes a basic helix-loop-helix protein that directly activates transcription of structural anthocyanin genes. Plant Cell 12:1619–1631
Starkevič P, Paukštytė J, Kazanavičiūtė V, Denkovskienė E, Stanys V, Bendokas V (2015) Expression and anthocyanin biosynthesis-modulating potential of sweet cherry (Prunus avium L.) MYB10 and bHLH genes. PLoS ONE 10:e0126991
Sun SS, Gugger PF, Wang QF, Chen JM (2016) Identification of a R2R3-MYB gene regulating anthocyanin biosynthesis and relationships between its variation and flower color difference in lotus (Nelumbo Adans.). PeerJ 4:e2369
Suzuki K, Suzuki T, Nakatsuka T, Dohra H, Yamagishi M, Matsuyama K (2016) RNA-seq-based evaluation of bicolor tepal pigmentation in Asiatic hybrid lilies (Lilium spp.). BMC Genom 17:611
Takos AM, Jaffé FW, Jacob SR, Bogs J, Robinson SP, Walker AR (2006) Light-induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples. Plant Physiol 142:1216–1232
Telias A, Lin-Wang K, Stevenson DE, Cooney JM, Hellens RP, Allan AC (2011) Apple skin patterning is associated with differential expression of MYB10. BMC Plant Biol 11:93
Terrier N, Torregrosa L, Ageorges A, Vialet S, Verriès C, Cheynier V (2009) Ectopic expression of VvMybPA2 promotes proanthocyanidin biosynthesis in grapevine and suggests additional targets in the pathway. Plant Physiol 149:1028–1041
This P, Lacombe T, Cadle-Davidson M, Owens CL (2007) Wine grape (Vitis vinifera L.) color associates with allelic variation in the domestication gene VvmybA1. Theor Appl Genet 114:723–730
Tian J, Peng Z, Zhang J, Song TT, Wan HH, Zhang ML (2015) McMYB10 regulates coloration via activating McF3′H and later structural genes in ever-red leaf crabapple. Plant Biotechnol J 13:948–961
Vimolmangkang S, Han Y, Wei G, Korban SS (2013) An apple MYB transcription factor, MdMYB3, is involved in regulation of anthocyanin biosynthesis and flower development. BMC Plant Biol 13:176
Walker AR, Elizabeth L, Jochen B, Debra AJ, Mc D, Mark RT (2007) White grapes arose through the mutation of two similar and adjacent regulatory genes. Plant J 49:772–785
Wu XM, Lim SH, Yang WC (2003) Characterization, expression and phylogenetic study of R2R3-MYB genes in orchid. Plant Mol Biol 51:959–972
Xie QL, Hu ZL, Zhang YJ, Tian SB, Wang ZJ, Zhao ZP (2014) Accumulation and molecular regulation of anthocyanin in purple tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee). J Agric Food Chem 62:7813–7821
Xu ZS, Feng K, Que F, Wang F, Xiong AS (2017) A MYB transcription factor, DcMYB6, is involved in regulating anthocyanin biosynthesis in purple carrot taproots. Sci Rep 7:4532
Yamagishi M (2011) Oriental hybrid lily Sorbonne homologue of LhMYB12 regulates anthocyanin biosyntheses in flower tepals and tepal spots. Mol Breed 28:381–389
Yamagishi M (2016) A novel R2R3-MYB transcription factor regulates light-mediated floral and vegetative anthocyanin pigmentation patterns in Lilium regale. Mol Breed 36:1–14
Yamagishi M, Shimoyamada Y, Nakatsuka T, Masuda K (2010) Two R2R3-MYB genes, homologs of Petunia AN2, regulate anthocyanin biosyntheses in flower tepals, tepal spots and leaves of Asiatic hybrid lily. Plant Cell Physiol 51:463–474
Yamagishi M, Yoshida Y, Nakayama M (2012) The transcription factor LhMYB12 determines anthocyanin pigmentation in the tepals of Asiatic hybrid lilies (Lilium spp.) and regulates pigment quantity. Mol Breed 30:913–925
Yamagishi M, Toda S, Tasaki K (2014) The novel allele of the LhMYB12 gene is involved in splatter-type spot formation on the flower tepals of Asiatic hybrid lilies (Lilium spp.). New Phytol 201:1009–1020
Yoshida K, Ma D, Constabel CP (2015) The MYB182 protein downregulates proanthocyanidin and anthocyanin biosynthesis in poplar by repressing both structural and regulatory flavonoid genes. Plant Physiol 167:693–710
Yuan Y, Chiu LW, Li L (2009) Transcriptional regulation of anthocyanin biosynthesis in red cabbage. Planta 230:1141–1153
Yuan YW, Sagawa JM, Frost L, Vela JP, Bradshaw HD (2014) Transcriptional control of floral anthocyanin pigmentation in monkeyflowers (Mimulus). New Phytol 204:1013–1027
Yuan YW, Rebocho AB, Sagawa JM, Stanley LE, Bradshaw HD (2016) Competition between anthocyanin and flavonol biosynthesis produces spatial pattern variation of floral pigments between Mimulus species. Proc Natl Acad Sci USA 113:2448–2453
Zhang YJ, Hu ZL, Chu GH, Huang C, Tian SB, Zhao ZP (2014) Anthocyanin accumulation and molecular analysis of anthocyanin biosynthesis-associated genes in eggplant (Solanum melongena L.). J Agric Food Chem 62:2906–2912
Zhang Y, Hu Z, Zhu M, Zhu Z, Wang Z, Tian S (2015a) Anthocyanin accumulation and molecular analysis of correlated genes in Purple Kohlrabi (Brassica oleracea var. gongylodes L.). J Agric Food Chem 63:4160–4169
Zhang Z, Li DW, Jin JH, Yin YX, Zhang HX, Chai WG (2015b) VIGS approach reveals the modulation of anthocyanin biosynthetic genes by CaMYB in chili pepper leaves. Front Plant Sci 6:500
Zhang Y, Chu G, Hu Z, Gao Q, Cui B, Tian S (2016) Genetically engineered anthocyanin pathway for high health-promoting pigment production in eggplant. Mol Breed 36:54
Zhang Q, Hao R, Xu Z, Yang W, Wang J, Cheng T (2017) Isolation and functional characterization of a R2R3-MYB regulator of Prunus mume anthocyanin biosynthetic pathway. Plant Cell Tiss Organ Cult 131:417–429
Zhou Y, Zhou H, Lin-Wang K, Vimolmangkang S, Espley RV, Wang L (2014) Transcriptome analysis and transient transformation suggest an ancient duplicated MYB transcription factor as a candidate gene for leaf red coloration in peach. BMC Plant Biol 14:388
Zhou H, Lin-Wang K, Wang H, Gu C, Dare AP, Espley RV (2015) Molecular genetics of blood-fleshed peach reveals activation of anthocyanin biosynthesis by NAC transcription factors. Plant J 82:105–121
Zhou H, Peng Q, Zhao J, Owiti A, Ren F, Liao L (2016) Multiple R2R3-MYB transcription tactors involved in the regulation of anthocyanin accumulation in peach flower. Front Plant Sci 7:1557
Zhu L, Shan H, Chen S, Jiang J, Gu C, Zhou G (2013) The heterologous expression of the chrysanthemum R2R3-MYB transcription factor CmMYB1 alters lignin composition and represses flavonoid synthesis in Arabidopsis thaliana. PLoS ONE 8:e65680
Zimmermann IM, Heim MA, Weisshaar B, Uhrig JF (2004) Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins. Plant J 40:22–34
Zuluaga DL, Gonzali S, Loreti E, Pucciariello C, Degl’Innocenti ED, Guidi L (2008) Arabidopsis thaliana MYB75/PAP1 transcription factor induces anthocyanin production in transgenic tomato plants. Funct Plant Biol 35:606–618
Zvi MMB, Shklarman E, Masci T, Kalev H, Debener T, Shafir S (2012) PAP1 transcription factor enhances production of phenylpropanoid and terpenoid scent compounds in rose flowers. New Phytol 195:334–335
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This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01368505)” Rural Development Administration, Republic of Korea. We sincerely thank to Dr. Khaing Thandar Soe (Seoul National University, South Korea) for her kind assistance in collection of difficult-to-access articles.
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AHN collected the literature and wrote the manuscript; CKK advised and assisted with the writing of the manuscript.
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Naing, A.H., Kim, C.K. Roles of R2R3-MYB transcription factors in transcriptional regulation of anthocyanin biosynthesis in horticultural plants. Plant Mol Biol 98, 1–18 (2018). https://doi.org/10.1007/s11103-018-0771-4
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DOI: https://doi.org/10.1007/s11103-018-0771-4