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Overexpression of microRNA828 reduces anthocyanin accumulation in Arabidopsis

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Abstract

Plant microRNAs (miRNAs) are RNAs of 20–22 nucleotides in length with sequence complementarities to specific mRNAs that are targeted for either cleavage or translational repression. They play critical regulatory roles in plant growth and development. In this study, the functionality of miR828 in the regulation of anthocyanin biosynthesis has been investigated. RNA blotting analysis has confirmed the presence of a conserved 22 nucleotide miR828 in both dicot and monocot plants. Moreover, it has revealed that miR828 is constitutively expressed in different tissues of Arabidopsis. Subsequently, a 35S:pre-miR828 construct has been created and transformed into Arabidopsis. Expression analysis has shown that levels of pre-miR828 and mature miR828 transcripts are increased, while levels of MYB75, MYB90, and MYB113 transcripts, encoding MYB transcription factors that positively regulate anthocyanin biosynthesis, are repressed in transgenic plants. As a result, anthocyanin levels are reduced, as are transcription levels of genes that are directly involved in anthocyanin biosynthesis, including PAL, CHS, CHI, F3H, F3′H, DFR, and LDOX. In addition, overexpression of miR828 in Arabidopsis inhibits transcription of yet another MYB factor, MYB82, indicating that MYB82 is likely to be involved in the anthocyanin biosynthesis pathway.

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Abbreviations

PAL:

Phenylalanine ammonialyase

CHI:

Chalcone isomerase

CHS:

Chalcone synthase

F3H:

Flavanone 3-hydroxylase

F3′H:

Flavonoid 3′-hydroxylase

DFR:

Dihydroflavonol reductase

LDOX:

Leucoanthocyandin dioxygenase

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (30971557 to Y.Y), and Chinese Universities Scientific Fund (2011SCU11107).

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Authors and Affiliations

  1. Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, Sichuan, People’s Republic of China

    Fengxi Yang, Yi Yang & Zhibin Liu

  2. West China School of Pharmacy, Sichuan University, Chengdu, 610064, Sichuan, People’s Republic of China

    Jing Cai

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  1. Fengxi Yang
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  2. Jing Cai
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  3. Yi Yang
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Correspondence to Zhibin Liu.

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Yang, F., Cai, J., Yang, Y. et al. Overexpression of microRNA828 reduces anthocyanin accumulation in Arabidopsis . Plant Cell Tiss Organ Cult 115, 159–167 (2013). https://doi.org/10.1007/s11240-013-0349-4

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  • DOI: https://doi.org/10.1007/s11240-013-0349-4

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