Abstract
Metal-tolerance proteins (MTPs) are divalent cation transporters and play fundamental roles in plant metal tolerance and ion homeostasis. Despite that, a systematic investigation of MTPs in Pear is still lacking. In this study, 18 MTPs were identified from the pear genome database and renamed as PbrMTP1–18. They were phylogenetically divided into four clusters (Zn-cation diffusion facilitator proteins [CDFs], cation efflux domain, CzcD, and FieF) and further subdivided into six groups (G1, G5, G6, G7, G8, and G09). Characterization analysis revealed that most MTPs were plasma-membrane-located hydrophobic proteins. Motif and exon/intron analysis showed that members in the same group contained similar conserved motifs and gene structures. Moreover, eight pairs of segmental-like duplication events were found in the pear genome. Gene ontology (GO) and cis-elements revealed that PbrMTP genes are highly responsive to stresses and participate in developmental activities via hormonal regulation. Tissue-specific expression analysis disclosed that PbrMTPs could be involved in organ development. Comprehensive expression of PbrMTPs in 5 pear cultivars at different fruit developmental stages unveiled their crucial role. The PbrMTP04 was highly expressed at all the fruit developmental stages and could be involved in pear fruit setting and ripening. Our study, for the first time, highlighted the role of MTP genes during fruit developmental stages. We believe our work would provide an impetus for future pear breeding programs.
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Acknowledgements
This work was supported by the Open Project Program of the State Key Laboratory of Crop Biology (Grant No. 2021KF06) and the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (Grant No. 2021L101).
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Riuru Xu: Writing-original draft, Data analysis, Formal analysis. Ahmad Ali: Data analysis, Investigation. Ying Li: Writing-review & editing. Xiao Zhang: Data analysis. Li Su: Investigation, Data analysis. Rahat Sharif: Writing-review & editing. Xinxin Feng and Baopeng Ding: Conceived and designed the experiments.
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Xu, R., Ali, A., Li, Y. et al. Transcriptome-Wide Analysis Revealed the Influential Role of PbrMTP (Metal Tolerance Protein) in the Growth and Fruit Development of Chinese White Pear. J Plant Growth Regul (2023). https://doi.org/10.1007/s00344-023-11107-8
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DOI: https://doi.org/10.1007/s00344-023-11107-8