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Adventitious Rooting and Browning are Differentially Controlled by Auxin in Rooting-Recalcitrant Elegia capensis (Burm. f.) Schelpe

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Abstract

Industrial propagation of horticultural species involves adventitious root (AR) induction, which is difficult to establish in rooting-recalcitrant species. One of these recalcitrant species is Elegia capensis (Burm. f.) Schelpe. This monocotyledonous species was used to investigate the correlation between tissue browning and AR formation. To evaluate the root-inducing potential of various auxin treatments, a test system using stem segments of in vitro grown Elegia shoots was developed. In addition to exogenous auxin treatments, the effect of more sucrose in the medium and the effect of different light quality were evaluated. The auxins IAA, IBA, NAA, and 2,4-D did not induce roots under standard light conditions, which confirmed the strong rooting-recalcitrance of E. capensis. However, in the dark, IBA successfully induced roots in 20–40 % of the segments, depending on the auxin concentration applied. Excised stem segments showed browning upon incubation with auxins. Browning was dependent on auxin type and concentration, yet it did not correlate with the capacity to form roots because AR formation was observed on brown stem segments. We therefore conclude that browning does not cause rooting-recalcitrance in E. capensis.

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Acknowledgments

This research was supported by the Agency for Innovation by Science and Technology in Flanders (IWT 83231) and a fellowship from Ghent University (BOF 01J11408). Plants were supplied by Oprins Plant NV. (Rijkevorsel, Belgium). We thank Valerie Verschoote for technical assistance.

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  1. Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000, Ghent, Belgium

    Inge Verstraeten & Danny Geelen

  2. Department of Plant Systems Biology, VIB (Flanders Institute for Biotechnology), Technologiepark 927, 9052, Ghent, Belgium

    Inge Verstraeten

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  1. Inge Verstraeten
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  2. Danny Geelen
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Correspondence to Danny Geelen.

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Verstraeten, I., Geelen, D. Adventitious Rooting and Browning are Differentially Controlled by Auxin in Rooting-Recalcitrant Elegia capensis (Burm. f.) Schelpe. J Plant Growth Regul 34, 475–484 (2015). https://doi.org/10.1007/s00344-015-9482-0

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