Abstract
Key message
Over expression of MsSPL12 improved alfalfa salt tolerance by reducing Na+ accumulation and increasing antioxidant enzyme activity and regulating down-stream gene expression.
Abstract
Improvement of salt tolerance is one of the major goals in alfalfa breeding. Here, we demonstrated that MsSPL12, an alfalfa transcription factor gene highly expressed in the stem cells, plays a positive role in alfalfa salt tolerance. MsSPL12 is localized in the nucleus and shows transcriptional activity in the presence of its C-terminus. To investigate MsSPL12 function in plant response to salt stress, we generated transgenic plants overexpressing either MsSPL12 or a chimeric MsSPL12-SRDX gene that represses the function of MsSPL12 by using the Chimeric REpressor gene-Silencing Technology (CRES-T), and observed that overexpression of MsSPL12 increased the salt tolerance of alfalfa transgenic plants associated with an increase in K+/Na+ ratio and relative water content (RWC) under salt stress treatment, but a reduction in electrolyte leakage (EL), reactive oxygen species (ROS), malondialdehyde (MDA), and proline (Pro) compared to wild type (WT) plants. However, transgenic plants overexpressing MsSPL12-SRDX showed an inhibited plant growth and a reduced salt tolerance. RNA-sequencing and quantitative real-time PCR analyses revealed that MsSPL12 affected the expression of plant abiotic resistance-related genes in multiple physiological pathways. The potential MsSPL12-mediated regulatory pathways based on the differentially expressed genes between the MsSPL12 overexpression transgenics and WT controls were predicted. In summary, our study proves that MsSPL12 is a positive regulator in alfalfa salt tolerance and can be used as a new candidate for manipulation to develop forage crops with enhanced salt tolerance.
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Data availability
The datasets generated during the current study are available from the corresponding author upon reasonable request. The RNA sequencing data for this research have been deposited in the NCBI Sequence Read Archive under accession code PRJNA997792.
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We thank Dr. Hong Luo of Clemson University for his help in proofreading the manuscript.
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The work was supported by the National Natural Science Foundation of China (31971755).
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W.Z and S.L conceived and designed the research. S.L, Y.L and J.Y, conducted the experiments. S.L analyzed data and drafted the manuscript, W.Z revised the manuscript. All authors read and approved the manuscript.
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Lin, S., Yang, J., Liu, Y. et al. MsSPL12 is a positive regulator in alfalfa (Medicago sativa L.) salt tolerance. Plant Cell Rep 43, 101 (2024). https://doi.org/10.1007/s00299-024-03175-1
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DOI: https://doi.org/10.1007/s00299-024-03175-1