Abstract
Key message
Hormone treatment enhanced the content of osmotic substances under high-temperature conditions. The effect of ABA and BR treated separately is better than treated together.
Abstract
To determine the effect of abscisic acid (ABA), brassinolide (BR) and ABA + BR on grape quality under high-temperature stress, various metabolites were analyzed. Compared with the control (CK), DL-tryptophan, d-raffinose, geniposidic acid, dodecanedioic acid and polyphenols were found to be higher after ABA treatment. After BR treatment, amino acids and poricoic acid B were higher than in CK. And carbohydrates and amino acids were up-regulated after ABA + BR treatment. BR and ABA + BR treatment also induced higher endogenous ABA and epibrassinolide contents. In addition, treated grape had higher soluble solid concentrations and soluble sugar content, and delayed the degradation of middle lamella and microfibrils. Antioxidant and heat shock-related genes were examined, which significantly increased in treated grape. The finding of this study suggested that ABA, BR and ABA + BR are very useful for alleviating high-temperature damage by increasing the accumulation of osmotic adjustment substances, and endogenous hormones content.
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Acknowledgements
We would like to express our gratitude to Ruiping Tian from the State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, for helping me analyze GC–MS data.
Funding
This work was supported by the national key research and development program (2019YFD1002500), National Natural Science Foundation of China (31872938, 31872047, 31672131), Xinjiang United fund (CXFZ202016), Natural science research project of colleges and universities in Jiangsu Province (20KJD210001), Jiangsu Natural Science Foundation (BK20201176).
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LJH and DTY treated the samples and conducted data analysis and drafted the manuscript. ZT and ZYP helped data analysis. FJG helped to revise the manuscript. YK helped to draft the manuscript. DTY participated in the design of the study. JHF designed and coordinated this study and revised the manuscript. All authors read and approved the final manuscript.
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Communicated by Zhanyuan Jon Zhang.
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Lv, J., Dong, T., Zhang, Y. et al. Metabolomic profiling of brassinolide and abscisic acid in response to high-temperature stress. Plant Cell Rep 41, 935–946 (2022). https://doi.org/10.1007/s00299-022-02829-2
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DOI: https://doi.org/10.1007/s00299-022-02829-2