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Piriformospora indica confers salinity tolerance on tomato (Lycopersicon esculentum Mill.) through amelioration of nutrient accumulation, K+/Na+ homeostasis and water status

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Piriformospora indica confers salt tolerance in tomato seedlings by increasing the uptake of nutrients such as N, P and Ca, improving K+/Na+ homoeostasis by regulating the expression of NHXs, SOS1 and CNGC15 genes, maintaining water status by regulating the expression of aquaporins.

Abstract

Piriformospora indica, an endophytic basidiomycete, has been shown to increase the growth and improve the plants tolerance to stressful conditions, especially salinity, by establishing the arbuscular mycorrhiza-like symbiotic relationship in various plant hosts. In the present research, the effect of NaCl treatment (150 mM) and P. indica inoculation on growth, accumulation of nutrients, the transcription level of genes involved in ionic homeostasis (NHXs, SOS1 and CNGC15) and regulating water status (PIP1;2, PIP2;4, TIP1;1 and TIP2;2) in roots and leaves of tomato seedlings were investigated. The P. indica improved the uptake of N, P, Ca and K, and reduced Na accumulation, and had no significant effect on Cl accumulation in roots and leaves. The endophytic fungus also increased in K+/Na+ ratio in roots and leaves of tomato by regulating the expression of NHX isoforms and upregulating SOS1 and CNGC15 expression. Salinity stress increased the transcription of PIP2;4 gene and reduced the transcription of PIP1;2, TIP1;1 and TIP2;2 genes compared to the control treatment. However, P. indica inoculation upregulated the expression of PIP1;2 and PIP2;4 genes versus non-inoculated plants but did not have a significant effect on TIP1;1 and TIP2;2 expression. These results conclude that the positive effects of P. indica on nutrients accumulation, ionic homeostasis and water status lead to the increased salinity tolerance and the improved plant growth under NaCl treatment.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Islamic Republic of Iran

    Abazar Ghorbani & Seyed Mehdi Razavi

  2. Department of Agronomy, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Science and Natural Resources University, Sari, Iran

    Vali Ollah Ghasemi Omran & Hemmatollah Pirdashti

  3. Microbial Biotechnology Department, College of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran

    Mojtaba Ranjbar

Authors
  1. Abazar Ghorbani
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  2. Vali Ollah Ghasemi Omran
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  3. Seyed Mehdi Razavi
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  4. Hemmatollah Pirdashti
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  5. Mojtaba Ranjbar
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Correspondence to Abazar Ghorbani or Vali Ollah Ghasemi Omran.

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Communicated by Prakash P. Kumar.

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Ghorbani, A., Omran, V.O.G., Razavi, S.M. et al. Piriformospora indica confers salinity tolerance on tomato (Lycopersicon esculentum Mill.) through amelioration of nutrient accumulation, K+/Na+ homeostasis and water status. Plant Cell Rep 38, 1151–1163 (2019). https://doi.org/10.1007/s00299-019-02434-w

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  • DOI: https://doi.org/10.1007/s00299-019-02434-w

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