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Effect of amphiphilic phosphorous dendrons on the conformation, secondary structure, and zeta potential of albumin and thrombin

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Abstract

Amphiphilic dendrons are highly branched synthetic polymeric soft nanoparticles that possess properties of both dendrimers and micelles. Such properties allow dendrons to become promising delivery systems for targeted delivery. While much attention is focused on the design and biological effect of a drug-delivery system, the nature of its interaction with surrounding tissues—their biocompatibility—is crucial for further optimization. The biocompatibility and biological effects of some amphiphilic phosphorus dendrons on human serum albumin and thrombin are investigated in this report. The results from protein fluorescence, circular dichroism, and zeta potential experiments showed that the dendrons of the first generation have a lesser impact on protein molecules than the dendrons of the second generation and therefore tend to be more biocompatible.

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Acknowledgements

This work was supported by the Polish National Agency for Academic Exchange, grant EUROPARTNER, No. PPI/APM/2018/1/00007/U/001; by the Slovak Research and Development Agency, grant APVV SK-BY-RD-19-0019; by the Belarusian Republican Foundation for Fundamental Research and State Committee of Science and Technology of Belarus, grants B20SLKG-002, B21KORG-001, B21TUB-001, B21RM-045, B21M-001. J.P. M thanks CNRS for financial support. S. M. was granted by FCT-Fundação para a Ciência e a Tecnologia (Base Fund UIDB/00674/2020 and Programmatic Fund UIDP/00674/2020, Portuguese Government Funds) and ARDITI-Agência Regional para o Desenvolvimento da Investigação Tecnologia e Inovação through the project M1420-01-0145-FEDER-000005-CQM + (Madeira 14–20 Program).

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Authors and Affiliations

  1. Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Akademičnaja 27, 220072, Minsk, Belarus

    Maria Terehova & Dzmitry Shcharbin

  2. Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236, Lodz, Poland

    Jakub Magiera, Maksim Ionov & Maria Bryszewska

  3. Laboratoire de Chimie de Coordination du CNRS, 205 route de Narbonne, 31077, Toulouse Cedex 4, France

    Jieru Qiu & Jean-Pierre Majoral

  4. Université Toulouse, 118 route de Narbonne, 31077, Toulouse Cedex 4, France

    Jieru Qiu & Jean-Pierre Majoral

  5. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China

    Xiangyang Shi

  6. CQM-Centro de Química da Madeira, MMRG, Universidade da Madeira, Campus da Penteada, 9020-105, Funchal, Portugal

    Xiangyang Shi & Serge Mignani

  7. CNRS UMR 860, Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologique, PRES Sorbonne Paris Cité, Université Paris Descartes, 45, rue des Saints Peres, 75006, Paris, France

    Serge Mignani

  8. Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina F1, 842 48, Bratislava, Slovakia

    Iveta Waczulikova

Authors
  1. Maria Terehova
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  2. Jakub Magiera
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  3. Jieru Qiu
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  5. Xiangyang Shi
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  6. Serge Mignani
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  7. Maksim Ionov
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  8. Iveta Waczulikova
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  9. Maria Bryszewska
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  10. Dzmitry Shcharbin
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Contributions

Conceptualization was conducted by MT, JM, JQ, JPM, XS, SM, MI, IW, MB, DS; Nanoparticles were prepared by JQ, JPM; Experiments were carried out by MT, JM; Formal analysis and investigation were provided by MT, JM, MI, IW, MB, DS; Original draft was prepared by MT, DS; Review and editing was provided by JPM, MI, IW, DS; Funding was acquised by JPM, SM, IW, MB, DS; Supervision was provided by JPM, XS, SM, MI, IW, MB, DS.

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Correspondence to Maria Terehova.

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Terehova, M., Magiera, J., Qiu, J. et al. Effect of amphiphilic phosphorous dendrons on the conformation, secondary structure, and zeta potential of albumin and thrombin. Polym. Bull. 80, 9181–9193 (2023). https://doi.org/10.1007/s00289-022-04512-8

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  • DOI: https://doi.org/10.1007/s00289-022-04512-8

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