Abstract
Escherichia coli has been a robust host strain for much biological research, in particular, research in metabolic engineering, protein engineering, and heterologous gene expression. In this mini review, to understand bacterial hydrogen production by E. coli, the effect of glucose and glycerol metabolism on hydrogen production is compared, and the current approaches to enhance hydrogen production from glycerol as a substrate are reviewed. In addition, the argument from past to present on the functions of E. coli hydrogenases, hydrogenase 1, hydrogenase 2, hydrogenase 3, and hydrogenase 4 is summarized. Furthermore, based on the literature that the E. coli formate-hydrogen lyase is essential for bacterial hydrogen production via recombinant hydrogenases, research achievements from the past regarding heterologous production of hydrogenase are rethought.
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References
Abdel-Hamid AM, Attwood MM, Guest JR (2001) Pyruvate oxidase contributes to the aerobic growth efficiency of Escherichia coli. Microbiology 147(Pt 6):1483–1498. https://doi.org/10.1099/00221287-147-6-1483
Akhtar MK, Jones PR (2008a) Deletion of iscR stimulates recombinant clostridial Fe-Fe hydrogenase activity and H2-accumulation in Escherichia coli BL21(DE3). Appl Microbiol Biotechnol 78(5):853–862. https://doi.org/10.1007/s00253-008-1377-6
Akhtar MK, Jones PR (2008b) Engineering of a synthetic hydF-hydE-hydG-hydA operon for biohydrogen production. Anal Biochem 373(1):170–172. https://doi.org/10.1016/J.Ab.2007.10.018
Akhtar MK, Jones PR (2009) Construction of a synthetic YdbK-dependent pyruvate:H2 pathway in Escherichia coli BL21(DE3). Metab Eng 11(3):139–147. https://doi.org/10.1016/j.ymben.2009.01.002
Andrews SC, Berks BC, McClay J, Ambler A, Quail MA, Golby P, Guest JR (1997) A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system. Microbiology 143(11):3633–3647. https://doi.org/10.1099/00221287-143-11-3633
Angelides KJ, Akiyama SK, Hammes GG (1979) Subunit stoichiometry and molecular weight of the pyruvate dehydrogenase multienzyme complex from Escherichia coli. Proc Natl Acad Sci U S A 76(7):3279–3283. https://doi.org/10.1073/pnas.76.7.3279
Armaroli N, Balzani V (2011) The hydrogen issue. ChemSusChem 4(21–36):21–36. https://doi.org/10.1002/cssc.201000182
Axley MJ, Grahame DA, Stadtman TC (1990) Escherichia coli formate-hydrogen lyase. Purification and properties of the selenium-dependent formate dehydrogenase component. J Biol Chem 265(30):18213–18218
Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:2006.0008
Bagramyan K, Trchounian A (2003) Structural and functional features of formate hydrogen lyase, an enzyme of mixed-acid fermentation from Escherichia coli. Biochemistry (Mosc) 68(11):1159–1170. https://doi.org/10.1023/B:BIRY.0000009129.18714.a4
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y (1997) The complete genome sequence of Escherichia coli K-12. Science 277(5331):1453–1474. https://doi.org/10.1126/science.277.5331.1453
Bouvet OM, Lenormand P, Carlier JP, Grimont PA (1994) Phenotypic diversity of anaerobic glycerol dissimilation shown by seven enterobacterial species. Res Microbiol 145(2):129–139. https://doi.org/10.1016/0923-2508(94)90006-X
Bouvet OM, Lenormand P, Ageron E, Grimont PA (1995) Taxonomic diversity of anaerobic glycerol dissimilation in the Enterobacteriaceae. Res Microbiol 146(4):279–290. https://doi.org/10.1016/0923-2508(96)81051-5
Chaudhary N (2011) Biosynthesis of ethanol and hydrogen by glycerol fermentation using Escherichia coli. Adv Chem Eng Sci 1(3):83–89. https://doi.org/10.4236/aces.2011.13014
Chittibabu G, Nath K, Das D (2006) Feasibility studies on the fermentative hydrogen production by recombinant Escherichia coli BL-21. Process Biochem 41(3):682–688. https://doi.org/10.1016/j.procbio.2005.08.020
Clomburg JM, Gonzalez R (2013) Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals. Trends Biotechnol 31(1):20–28. https://doi.org/10.1016/j.tibtech.2012.10.006
Das D, Veziroglu TN (2001) Hydrogen production by biological processes: a survey of literature. Int J Hydrog Energy 26(1):13–28. https://doi.org/10.1016/S0360-3199(00)00058-6
Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97(12):6640–6645. https://doi.org/10.1073/pnas.120163297
Dharmadi Y, Murarka A, Gonzalez R (2006) Anaerobic fermentation of glycerol by Escherichia coli: a new platform for metabolic engineering. Biotechnol Bioeng 94(5):821–829. https://doi.org/10.1002/bit.21025
Dunn S (2002) Hydrogen futures: toward a sustanable energy system. Int J Hydrog Energy 27(3):235–264. https://doi.org/10.1016/S0360-3199(01)00131-8
Forzi L, Sawers RG (2007) Maturation of [NiFe]-hydrogenases in Escherichia coli. Biometals 20(3–4):565–578. https://doi.org/10.1007/s10534-006-9048-5
Ghosh D, Bisaillon A, Hallenbeck PC (2013) Increasing the metabolic capacity of Escherichia coli for hydrogen production through heterologous expression of the Ralstonia eutropha SH operon. Biotechnol Biofuels 6(1):122. https://doi.org/10.1186/1754-6834-6-122
Gil R, Latorre A (2012) Factors behind junk DNA in bacteria. Genes (Basel) 3(4):634–650. https://doi.org/10.3390/genes3040634
Gonzalez R, Murarka A, Dharmadi Y, Yazdani SS (2008) A new model for the anaerobic fermentation of glycerol in enteric bacteria: trunk and auxiliary pathways in Escherichia coli. Metab Eng 10(5):234–245. https://doi.org/10.1016/j.ymben.2008.05.001
Hansel A, Lindblad P (1998) Toward optimization of cyanobacteria as biotechnologically relevant producers of molecular hydrogen, a clean and renewable energy source. Appl Microbiol Biotechnol 50(2):153–160. https://doi.org/10.1007/s002530051270
Holtman CK, Pawlyk AC, Meadow ND, Pettigrew DW (2001) Reverse genetics of Escherichia coli glycerol kinase allosteric regulation and glucose control of glycerol utilization in vivo. J Bacteriol 183(11):3336–3344. https://doi.org/10.1128/JB.183.11.3336-3344.2001
Hu H, Wood TK (2010) An evolved Escherichia coli strain for producing hydrogen and ethanol from glycerol. Biochem Biophys Res Commun 391(1):1033–1038. https://doi.org/10.1016/j.bbrc.2009.12.013
Jo BH, Cha HJ (2015) Activation of formate hydrogen-lyase via expression of uptake [NiFe]-hydrogenase in Escherichia coli BL21(DE3). Microb Cell Factories 14(1):151. https://doi.org/10.1186/s12934-015-0343-0
Kessel DG (2000) Global warming—facts, assessment, countermeasures. J Pet Sci Eng 26(1-4):157–168. https://doi.org/10.1016/S0920-4105(00)00030-9
Kim D, Han S, Kim S, Shin H (2006) Effect of gas sparging on continuous fermentative hydrogen production. Int J Hydrog Energy 31(15):2158–2169. https://doi.org/10.1016/j.ijhydene.2006.02.012
Kim JYH, Jo BH, Cha HJ (2010) Production of biohydrogen by recombinant expression of [NiFe]-hydrogenase 1 in Escherichia coli. Microb Cell Fact 9:54. https://doi.org/10.1186/1475-2859-9-54
Kitagawa M, Ara T, Arifuzzaman M, Ioka-Nakamichi T, Inamoto E, Toyonaga H, Mori H (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res 12(5):291–299. https://doi.org/10.1093/dnares/dsi012
Lee SY, Lee HJ, Park JM, Lee JH, Park JS, Shin HS, Kim YH, Min J (2010) Bacterial hydrogen production in recombinant Escherichia coli harboring a HupSL hydrogenase isolated from Rhodobacter sphaeroides under anaerobic dark culture. Int J Hydrogen Energy 35(3):1112–1116. https://doi.org/10.1016/j.ijhydene.2009.11.068
Lim JH, Jung GY (2017) A simple method to control glycolytic flux for the design of an optimal cell factory. Biotechnol Biofuels 10(1):160. https://doi.org/10.1186/s13068-017-0847-4
Ma K, Maeda T, You H, Shirai Y (2014) Open fermentative production of L-lactic acid with high optical purity by thermophilic Bacillus coagulans using excess sludge as nutrient. Bioresour Technol 151:28–35. https://doi.org/10.1016/j.biortech.2013.10.022
Maeda T, Wood TK (2008) Formate detection by potassium permanganate for enhanced hydrogen production in Escherichia coli. Int J Hydrog Energy 33(9):2409–2412. https://doi.org/10.1016/j.ijhydene.2008.02.054
Maeda T, Sanchez-Torres V, Wood TK (2007a) Enhanced hydrogen production from glucose by metabolically engineered Escherichia coli. Appl Microbiol Biotechnol 77(4):879–890. https://doi.org/10.1007/s00253-007-1217-0
Maeda T, Sanchez-Torres V, Wood TK (2007b) Escherichia coli hydrogenase 3 is a reversible enzyme possessing hydrogen uptake and synthesis activities. Appl Microbiol Biotechnol 76(5):1035–1042. https://doi.org/10.1007/s00253-007-1086-6
Maeda T, Vardar G, Self WT, Wood TK (2007c) Inhibition of hydrogen uptake in Escherichia coli by expressing the hydrogenase from the cyanobacterium Synechocystis sp. PCC 6803. BMC Biotechnol 7(1):25. https://doi.org/10.1186/1472-6750-7-25
Maeda T, Sanchez-Torres V, Wood TK (2008a) Metabolic engineering to enhance bacterial hydrogen production. Microb Biotechnol 1(1):30–39. https://doi.org/10.1111/j.1751-7915.2007.00003.x
Maeda T, Sanchez-Torres V, Wood TK (2008b) Protein engineering of hydrogenase 3 to enhance hydrogen production. Appl Microbiol Biotechnol 79(1):77–86. https://doi.org/10.1007/s00253-008-1416-3
Maeda T, Yoshimura T, Shimazu T, Shirai Y, Ogawa HI (2009) Enhanced production of lactic acid with reducing excess sludge by lactate fermentation. J Hazard Mater 168(2–3):656–663. https://doi.org/10.1016/j.jhazmat.2009.02.067
Maeda T, Sanchez-Torres V, Wood TK (2012) Hydrogen production by recombinant Escherichia coli strains. Microb Biotechnol 5(2):214–225. https://doi.org/10.1111/j.1751-7915.2011.00282.x
Maier JA, Ragozin S, Jeltsch A (2015) Identification, cloning and heterologous expression of active [NiFe]-hydrogenase 2 from Citrobacter sp. SG in Escherichia coli. J Biotechnol 199:1–8. https://doi.org/10.1016/j.jbiotec.2015.01.025
Mazumdar S, Clomburg JM, Gonzalez R (2010) Escherichia coli strains engineered for homofermentative production of D-lactic acid from glycerol. Appl Environ Microbiol 76(13):4327–4336. https://doi.org/10.1128/AEM.00664-10
McDowall JS, Hjersing MC, Palmer T, Sargent F (2015) Dissection and engineering of the Escherichia coli formate hydrogenlyase complex. FEBS Lett 589(20 Pt B):3141–3147. https://doi.org/10.1016/j.febslet.2015.08.043
Menon NK, Robbins J, Wendt JC, Shanmugam KT, Przybyla AE (1991) Mutational analysis and characterization of the Escherichia coli hya operon, which encodes [NiFe] hydrogenase 1. J Bacteriol 173(15):4851–4861. https://doi.org/10.1128/jb.173.15.4851-4861.1991
Menon NK, Chatelus CY, Dervartanian M, Wendt JC, Shanmugam KT, Peck HD Jr, Przybyla AE (1994) Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2. J Bacteriol 176(14):4416–4423. https://doi.org/10.1128/jb.176.14.4416-4423.1994
Mirzoyan S, Romero-Pareja PM, Coello MD, Trchounian A, Trchounian K (2017) Evidence for hydrogenase-4 catalyzed biohydrogen production in Escherichia coli. Int J Hydrog Energy 42(34):21697–21703. https://doi.org/10.1016/j.ijhydene.2017.07.126
Mishra J, Khurana S, Kumar N, Ghosh AK, Das D (2004) Molecular cloning, characterization, and overexpression of a novel [Fe]-hydrogenase isolated from a high rate of hydrogen producing Enterobacter cloacae IIT-BT 08. Biochem Biophys Res Commun 324(2):679–685. https://doi.org/10.1016/j.bbrc.2004.09.108
Mizuno O, Dinsdale R, Hawkes FR, Howkes DL, Noike T (2000) Enhancement of hydrogen production from glucose by nitrogen gas sparging. Bioresour Technol 73(1):59–65. https://doi.org/10.1016/S0960-8524(99)00130-3
Mohd Yasin NH, Fukuzaki M, Maeda T, Miyazaki T, Che Maail CMH, Ariffin H, Wood TK (2013) Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically-engineered Escherichia coli strain. Int J Hydrog Energy 38(25):10277–10283. https://doi.org/10.1016/j.ijhydene.2013.06.065
Mohd Yasin NH, Maeda T, Hu A, Yu C-P, Wood TK (2015) CO2 sequestration by methanogens in activated sludge for methane production. Appl Energy 142:426–434. https://doi.org/10.1016/j.apenergy.2014.12.069
Mohd Yasin NH, Ikegami A, Wood TK, Yu C-P, Haruyama T, Takriff MS, Maeda T (2017) Oceans as bioenergy pools for methane production using activated methanogens in waste sewage sludge. Appl Energy 202:399–407. https://doi.org/10.1016/j.apenergy.2017.05.171
Mohd Yusoff MZ, Maeda T, Sanchez-Torres V, Ogawa HI, Shirai Y, Hassan MA, Wood TK (2012) Uncharacterized Escherichia coli proteins YdjA and YhjY are related to biohydrogen production. Int J Hydrog Energy 37(23):17778–17787. https://doi.org/10.1016/j.ijhydene.2012.08.115
Mohd Yusoff MZ, Hashiguchi Y, Maeda T, Wood TK (2013) Four products from Escherichia coli pseudogenes increase hydrogen production. Biochem Biophys Res Commun 439(4):576–579. https://doi.org/10.1016/j.bbrc.2013.09.016
Murarka A, Dharmadi Y, Yazdani SS, Gonzalez R (2008) Fermentative utilization of glycerol by Escherichia coli and its implications for the production of fuels and chemicals. Appl Environ Microbiol 74(4):1124–1135. https://doi.org/10.1128/AEM.02192-07
Olajuyin AM, Yang M, Liu Y, Mu T, Tian J, Adaramoye OA, Xing J (2016) Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli. Bioresour Technol 214:653–659. https://doi.org/10.1016/j.biortech.2016.04.117
Penfold DW, Macaskie LE (2004) Production of H2 from sucrose by Escherichia coli strains carrying the pUR400 plasmid, which encodes invertase activity. Biotechnol Lett 26(24):1879–1883. https://doi.org/10.1007/s10529-004-6035-1
Peterson JR, Cole JA, Luthey-Schulten Z (2017) Parametric studies of metabolic cooperativity in Escherichia coli colonies: strain and geometric confinement effects. PLoS One 12(8):e0182570. https://doi.org/10.1371/journal.pone.0182570
Pinske C, Jaroschinsky M, Linek S, Kelly CL, Sargent F, Sawers RG (2015) Physiology and bioenergetics of [NiFe]-hydrogenase 2-catalyzed H2-consuming and H2-producing reactions in Escherichia coli. J Bacteriol 197(2):296–306. https://doi.org/10.1128/JB.02335-14
Quastel JH, Stephenson M (1925) Further observations on the anaerobic growth of bacteria. Biochem J 19(4):660–666. https://doi.org/10.1042/bj0190660
Rossmann R, Sawers G, Böck A (1991) Mechanism of regulation of the formate-hydrogenlyase pathway by oxygen, nitrate, and pH: definition of the formate regulon. Mol Microbiol 5(11):2807–2814. https://doi.org/10.1111/j.1365-2958.1991.tb01989.x
Sanchez-Torres V, Maeda T, Wood TK (2009) Protein engineering of the transcriptional activator FhlA to enhance hydrogen production in Escherichia coli. Appl Environ Microbiol 75(17):5639–5646. https://doi.org/10.1128/Aem.00638-09
Sanchez-Torres V, Mohd Yusoff MZ, Nakao C, Maeda T, Ogawa HI, Wood TK (2013) Influence of Escherichia coli hydrogenases on hydrogen fermentation from glycerol. Int J Hydrog Energy 38(10):3905–3912. https://doi.org/10.1016/j.ijhydene.2013.01.031
Sawers G (1994) The hydrogenases and formate dehydrogenases of Escherichia coli. Antonie Van Leeuwenhoek 66(1–3):57–88. https://doi.org/10.1007/BF00871633
Schlensog V, Lutz S, Böck A (1994) Purification and DNA-binding properties of FHLA, the transcriptional activator of the formate hydrogenlyase system from Escherichia coli. J Biol Chem 269(30):19590–19596
Self WT, Hasona A, Shanmugam KT (2004) Expression and regulation of a silent operon, hyf, coding for hydrogenase 4 isoenzyme in Escherichia coli. J Bacteriol 186(2):580–587. https://doi.org/10.1128/JB.186.2.580-587.2004
Seol E, Sekar BS, Raj SM, Park S (2016) Co-production of hydrogen and ethanol from glucose by modification of glycolytic pathways in Escherichia coli—from Embden-Meyerhof-Parnas pathway to pentose phosphate pathway. Biotechnol J 11(2):249–256. https://doi.org/10.1002/biot.201400829
Shams Yazdani S, Gonzalez R (2008) Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products. Metab Eng 10(6):340–351. https://doi.org/10.1016/j.ymben.2008.08.005
Suppmann B, Sawers G (1994) Isolation and characterization of hypophosphite-resistant mutants of Escherichia coli: identification of the FocA protein, encoded by the pfl operon, as a putative formate transporter. Mol Microbiol 11(5):965–982. https://doi.org/10.1111/j.1365-2958.1994.tb00375.x
Taifor AF, Zakaria MR, Mohd Yusoff MZ, Maeda T, Hassan MA, Shirai Y (2017) Elucidating substrate utilization in biohydrogen production from palm oil mill effluent by Escherichia coli. Int J Hydrog Energy 42(9):5812–5819. https://doi.org/10.1016/j.ijhydene.2016.11.188
Tran KT, Maeda T, Wood TK (2014) Metabolic engineering of Escherichia coli to enhance hydrogen production from glycerol. Appl Microbiol Biotechnol 98(10):4757–4770. https://doi.org/10.1007/s00253-014-5600-3
Tran KT, Maeda T, Sanchez-Torres V, Wood TK (2015) Beneficial knockouts in Escherichia coli for producing hydrogen from glycerol. Appl Microbiol Biotechnol 99(6):2573–2581. https://doi.org/10.1007/s00253-014-6338-7
Trchounian K, Trchounian A (2009) Hydrogenase 2 is most and hydrogenase 1 is less responsible for H2 production by Escherichia coli under glycerol fermentation at neutral and slightly alkaline pH. Int J Hydrog Energy 34(21):8839–8845. https://doi.org/10.1016/j.ijhydene.2009.08.056
Trchounian K, Trchounian A (2014a) Different role of focA and focB encoding formate channels for hydrogen production by Escherichia coli during glucose or glycerol fermentation. Int J Hydrog Energy 39(36):20987–20991. https://doi.org/10.1016/j.ijhydene.2014.10.074
Trchounian K, Trchounian A (2014b) Hydrogen producing activity by Escherichia coli hydrogenase 4 (hyf) depends on glucose concentration. Int J Hydrog Energy 39(30):16914–16918. https://doi.org/10.1016/j.ijhydene.2014.08.059
Trchounian K, Trchounian A (2015) Hydrogen production from glycerol by Escherichia coli and other bacteria: an overview and perspectives. Appl Ener 156:174–184. https://doi.org/10.1016/j.apenergy.2015.07.009
Trchounian K, Sanchez-Torres V, Wood TK, Trchounian A (2011) Escherichia coli hydrogenase activity and H2 production under glycerol fermentation at a low pH. Int J Hydrog Energy 36(7):4323–4331. https://doi.org/10.1016/j.ijhydene.2010.12.128
Trchounian K, Pinske C, Sawers RG, Trchounian A (2012) Characterization of Escherichia coli [NiFe]-hydrogenase distribution during fermentative growth at different pHs. Cell Biochem Biophys 62(3):433–440. https://doi.org/10.1007/s12013-011-9325-y
Trchounian K, Blbulyan S, Trchounian A (2013a) Hydrogenase activity and proton-motive force generation by Escherichia coli during glycerol fermentation. J Bioenerg Biomembr 45(3):253–260. https://doi.org/10.1007/s10863-012-9498-0
Trchounian K, Soboh B, Sawers RG, Trchounian A (2013b) Contribution of hydrogenase 2 to stationary phase H2 production by Escherichia coli during fermentation of glycerol. Cell Biochem Biophys 66(1):103–108. https://doi.org/10.1007/s12013-012-9458-7
Trchounian K, Sawers RG, Trchounian A (2017) Improving biohydrogen productivity by microbial dark- and photo- fermentations: novel data and future approaches. Renew Sust Energ Rev 80:1201–1216. https://doi.org/10.1016/j.rser.2017.05.149
Vardar-Schara G, Maeda T, Wood TK (2008) Metabolically-engineered bacteria for producing hydrogen via fermentation. Microb Biotechnol 1(2):107–125. https://doi.org/10.1111/j.1751-7915.2007.00009.x
Wells MA, Mercer J, Mott RA, Pereira-Medrano AG, Burja AM, Radianingtyas H, Wright PC (2011) Engineering a non-native hydrogen production pathway into Escherichia coli via a cyanobacterial [NiFe] hydrogenase. Metab Eng. https://doi.org/10.1016/j.ymben.2011.01.004
Yazdani SS, Gonzalez R (2007) Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Curr Opin Biotechnol 18(3):213–219. https://doi.org/10.1016/j.copbio.2007.05.002
Yoshida A, Nishimura T, Kawaguchi H, Inui M, Yukawa H (2006) Enhanced hydrogen production from glucose using ldh- and frd-inactivated Escherichia coli strains. Appl Microbiol Biotechnol 73(1):67–72. https://doi.org/10.1007/s00253-006-0456-9
Zhao X, Xing DF, Zhang L, Ren NQ (2010) Characterization and overexpression of a [FeFe]-hydrogenase gene of a novel hydrogen-producing bacterium Ethanoligenens harbinense. Int J Hydrog Energy 35(18):9598–9602. https://doi.org/10.1016/j.ijhydene.2010.06.098
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Maeda, T., Tran, K.T., Yamasaki, R. et al. Current state and perspectives in hydrogen production by Escherichia coli: roles of hydrogenases in glucose or glycerol metabolism. Appl Microbiol Biotechnol 102, 2041–2050 (2018). https://doi.org/10.1007/s00253-018-8752-8
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DOI: https://doi.org/10.1007/s00253-018-8752-8