Abstract
Ruminal microbiota (RM) were co-inoculated with anaerobic sludge (AS) at different ratios to study the digestion of rice straw in batch experiments. The CH4 yield reached 273.64 mL/g volatile solid (VS) at a co-inoculum ratio of 1:1. The xylanase and cellulase activities were 198.88–212.88 and 24.51–29.08 U/mL in co-inoculated samples, respectively, and were significantly different compared to the results for single inoculum (p < 0.05). Higher ratios of AS enhanced acetoclastic methanogenesis, and propionate accumulation could be the main reason for the longer lag phase observed in samples with a higher RM ratio. The microbial compositions were clearly altered after digestion. Fibrobacter, Ruminococcus and Butyrivibrio from the rumen did not settle in the co-inoculated system, whereas Clostridiales members became the main polysaccharide degraders. Microbial interactions involving hydrolytic bacteria and acetoclastic methanogens in the residue were considered to be significant for hydrolysis activities and methane production. Syntrophy involving propionate oxidizers with associated methanogens occurred in the liquid phase. Our findings provide a better understanding of the anaerobic digestion of rice straw that is driven by specific microbial populations.
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Acknowledgments
This study was funded by National Natural Science Foundation of China [grant numbers 21506076, 51678279, 21276114], the National Science and Technological Support of China [grant number 2014BAC25B01] and the Fundamental Research Funds for the Central Universities [grant number JUSRP11435].
The authors were also grateful to Enzyme Engineering Technology Center of Jiangsu for the help in rumen collection of this work.
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Deng, Y., Huang, Z., Zhao, M. et al. Effects of co-inoculating rice straw with ruminal microbiota and anaerobic sludge: digestion performance and spatial distribution of microbial communities. Appl Microbiol Biotechnol 101, 5937–5948 (2017). https://doi.org/10.1007/s00253-017-8332-3
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DOI: https://doi.org/10.1007/s00253-017-8332-3