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Reactor performances and microbial communities of biogas reactors: effects of inoculum sources

  • Bioenergy and Biofuels
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Abstract

Anaerobic digestion is a very complex process that is mediated by various microorganisms, and the understanding of the microbial community assembly and its corresponding function is critical in order to better control the anaerobic process. The present study investigated the effect of different inocula on the microbial community assembly in biogas reactors treating cellulose with various inocula, and three parallel biogas reactors with the same inoculum were also operated in order to reveal the reproducibility of both microbial communities and functions of the biogas reactors. The results showed that the biogas production, volatile fatty acid (VFA) concentrations, and pH were different for the biogas reactors with different inocula, and different steady-state microbial community patterns were also obtained in different biogas reactors as reflected by Bray-Curtis similarity matrices and taxonomic classification. It indicated that inoculum played an important role in shaping the microbial communities of biogas reactor in the present study, and the microbial community assembly in biogas reactor did not follow the niche-based ecology theory. Furthermore, it was found that the microbial communities and reactor performances of parallel biogas reactors with the same inoculum were different, which could be explained by the neutral-based ecology theory and stochastic factors should played important roles in the microbial community assembly in the biogas reactors. The Bray-Curtis similarity matrices analysis suggested that inoculum affected more on the microbial community assembly compared to stochastic factors, since the samples with different inocula had lower similarity (10–20 %) compared to the samples from the parallel biogas reactors (30 %).

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References

  • Angelidaki I, Sanders W (2004) Assessment of the anaerobic biodegradability of macropollutants. Reviews in Environ Sci Biotechnol 3(2):117–129. doi:10.1007/s11157-004-2502-3

    Article  CAS  Google Scholar 

  • APHA (1995) Standard methods for the examination of water and wastewater, 19th edn. American Public Health Association, New York, USA

    Google Scholar 

  • Ayarza J, Erijman L (2011) Balance of neutral and deterministic components in the dynamics of activated sludge floc assembly. Microb Ecol 61(3):486–495. doi:10.1007/s00248-010-9762-y

    Article  PubMed  Google Scholar 

  • Baptista JC, Davenport RJ, Donnelly T, Curtis TP (2008) The microbial diversity of laboratory-scale wetlands appears to be randomly assembled. Water Res 42(12):3182–3190. doi:10.1016/j.watres.2008.03.013

    Article  CAS  Google Scholar 

  • Bates ST, Berg-Lyons D, Caporaso JG, Walters WA, Knight R, Fierer N (2011) Examining the global distribution of dominant archaeal populations in soil. ISME J 5(5):908–917. doi:10.1038/ismej.2010.171

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Chase J (2003) Community assembly: when should history matter? Oecologia 136(4):489–498. doi:10.1007/s00442-003-1311-7

    Article  PubMed  Google Scholar 

  • Chave J (2004) Neutral theory and community ecology. Ecol Let 7(3):241–253. doi:10.1111/j.1461-0248.2003.00566.x

    Article  Google Scholar 

  • Cole JR, Wang Q, Cardenas E, Fish J, Chai B, Farris RJ, Kulam-Syed-Mohideen AS, McGarrell DM, Marsh T, Garrity GM, Tiedje JM (2009) The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. Nucl Acids Res 37:D141–D145. doi:10.1093/nar/gkn879

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Dhamodharan K, Kumar V, Kalamdhad AS (2015) Effect of different livestock dungs as inoculum on food waste anaerobic digestion and its kinetics. Bioresour Technol 180(0):237–241. doi:10.1016/j.biortech.2014.12.066

    Article  PubMed  CAS  Google Scholar 

  • Fargione J, Brown C, Tilman D (2003) Community assembly and invasion: an experimental test of neutral versus niche processes. Proc Nat Acad Sci USA 100:8916–8920

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Fernández A, Huang S, Seston S, Xing J, Hickey R, Criddle C, Tiedje J (1999) How stable is stable? Function versus community composition. Appl Environ Microbiol 65(8):3697–3704

    PubMed  PubMed Central  Google Scholar 

  • Fernandez AS, Hashsham SA, Dollhopf SL, Raskin L, Glagoleva O, Dazzo FB, Hickey RF, Criddle CS, Tiedje JM (2000) Flexible community structure correlates with stable community function in methanogenic bioreactor communities perturbed by glucose. Appl Environ Microbiol 66(9):4058–4067. doi:10.1128/aem.66.9.4058-4067.2000

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Fotidis IA, Karakashev D, Kotsopoulos TA, Martzopoulos GG, Angelidaki I (2013) Effect of ammonium and acetate on methanogenic pathway and methanogenic community composition. FEMS Microbiol Ecol 83(1):38–48. doi:10.1111/j.1574-6941.2012.01456.x

    Article  PubMed  CAS  Google Scholar 

  • Fotidis IA, Wang H, Fiedel NR, Luo G, Karakashev DB, Angelidaki I (2014) Bioaugmentation as a solution to increase methane production from an ammonia-rich substrate. Environ Sci Technol 48(13):7669–7676. doi:10.1021/es5017075

    Article  PubMed  CAS  Google Scholar 

  • Gu Y, Chen X, Liu Z, Zhou X, Zhang Y (2014) Effect of inoculum sources on the anaerobic digestion of rice straw. Bioresour Technol 158(0):149–155. doi:10.1016/j.biortech.2014.02.011

    Article  PubMed  CAS  Google Scholar 

  • Karakashev D, Batstone DJ, Angelidaki I (2005) Influence of environmental conditions on methanogenic compositions in anaerobic biogas reactors. Appl Environ Microbiol 71(1):331–338. doi:10.1128/aem.71.1.331-338.2005

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Krakat N, Schmidt S, Scherer P (2011) Potential impact of process parameters upon the bacterial diversity in the mesophilic anaerobic digestion of beet silage. Bioresour Technol 102(10):5692–5701. doi:10.1016/j.biortech.2011.02.108

    Article  PubMed  CAS  Google Scholar 

  • Kurtz JC, Devereux R, Barkay T, Jonas RB (1998) Evaluation of sediment slurry microcosms for modeling microbial communities in estuarine sediments. Environ Toxicol Chem 17(7):1274–1281. doi:10.1002/etc.5620170712

    Article  CAS  Google Scholar 

  • Lü F, Hao L, Guan D, Qi Y, Shao L, He P (2013) Synergetic stress of acids and ammonium on the shift in the methanogenic pathways during thermophilic anaerobic digestion of organics. Water Res 47(7):2297–2306. doi:10.1016/j.watres.2013.01.049

    Article  PubMed  CAS  Google Scholar 

  • Lee C, Kim J, Shin SG, Hwang S (2008) Monitoring bacterial and archaeal community shifts in a mesophilic anaerobic batch reactor treating a high-strength organic wastewater. FEMS Microbiol Ecol 65(3):544–554. doi:10.1111/j.1574-6941.2008.00530.x

    Article  PubMed  CAS  Google Scholar 

  • Lopes WS, Leite VD, Prasad S (2004) Influence of inoculum on performance of anaerobic reactors for treating municipal solid waste. Bioresour Technol 94(3):261–266. doi:10.1016/j.biortech.2004.01.006

    Article  PubMed  CAS  Google Scholar 

  • Lu L, Xing DF, Ren NQ (2012) Pyrosequencing reveals highly diverse microbial communities in microbial electrolysis cells involved in enhanced H-2 production from waste activated sludge. Water Res 46(7):2425–2434. doi:10.1016/j.watres.2012.02.005

    Article  PubMed  CAS  Google Scholar 

  • Luo G, Angelidaki I (2014) Analysis of bacterial communities and bacterial pathogens in a biogas plant by the combination of ethidium monoazide, PCR and Ion Torrent sequencing. Water Res 60:156–163. doi:10.1016/j.watres.2014.04.047

    Article  PubMed  CAS  Google Scholar 

  • Luo G, De Francisci D, Kougias P, Laura T, Zhu X, Angelidaki I (2015) New steady-state microbial community compositions and process performances in biogas reactors induced by temperature disturbances. Biotechnol Biofuels 8(1):3

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Luo G, Wang W, Angelidaki I (2013) Anaerobic digestion for simultaneous sewage sludge treatment and CO biomethanation: process performance and microbial ecology. Environ Sci Technol 47(18):10685–10693. doi:10.1021/es401018d

    PubMed  CAS  Google Scholar 

  • Nesbø CL, Kumaraswamy R, Dlutek M, Doolittle WF, Foght J (2010) Searching for mesophilic Thermotogales bacteria: “mesotogas” in the wild. Appl Environ Microbiol 76(14):4896–4900. doi:10.1128/aem.02846-09

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Nguyen T-AD, Han SJ, Kim JP, Kim MS, Oh YK, Sim SJ (2008) Hydrogen production by the hyperthermophilic eubacterium, Thermotoga neapolitana, using cellulose pretreated by ionic liquid. Int J Hydrogen Energ 33(19):5161–5168. doi:10.1016/j.ijhydene.2008.05.019

    Article  CAS  Google Scholar 

  • Nielsen HB, Mladenovska Z, Westermann P, Ahring BK (2004) Comparison of two-stage thermophilic (68 °C/55 °C) anaerobic digestion with one-stage thermophilic (55 °C) digestion of cattle manure. Biotechnol Bioeng 86(3):291–300. doi:10.1002/bit.20037

    Article  PubMed  CAS  Google Scholar 

  • Pagaling E, Strathdee F, Spears BM, Cates ME, Allen RJ, Free A (2014) Community history affects the predictability of microbial ecosystem development. ISME J 8(1):19–30. doi:10.1038/ismej.2013.150

    Article  PubMed  PubMed Central  Google Scholar 

  • Regueiro L, Spirito CM, Usack JG, Hospodsky D, Werner JJ, Angenent LT (2015) Comparing the inhibitory thresholds of dairy manure co-digesters after prolonged acclimation periods: Part 2—correlations between microbiomes and environment. Water Res. doi:10.1016/j.watres.2015.05.046

  • Rincón B, Borja R, González JM, Portillo MC, Sáiz-Jiménez C (2008) Influence of organic loading rate and hydraulic retention time on the performance, stability and microbial communities of one-stage anaerobic digestion of two-phase olive mill solid residue. Biochem Eng J 40(2):253–261. doi:10.1016/j.bej.2007.12.019

    Article  CAS  Google Scholar 

  • Riviere D, Desvignes V, Pelletier E, Chaussonnerie S, Guermazi S, Weissenbach J (2009) Towards the definition of a core of microorganisms involved in anaerobic digestion of sludge. ISME J 3:700–714

    Article  PubMed  Google Scholar 

  • Schluter A, Bekel T, Diaz NN, Dondrup M, Eichenlaub R, Gartemann KH, Krahn I, Krause L, Kromeke H, Kruse O, Mussgnug JH, Neuweger H, Niehaus K, Puhler A, Runte KJ, Szczepanowski R, Tauch A, Tilker A, Viehover P, Goesmann A (2008) The metagenome of a biogas-producing microbial community of a production-scale biogas plant fermenter analysed by the 454-pyrosequencing technology. J Biotechnol 136(1–2):77–90. doi:10.1016/j.jbiotec.2008.05.008

    Article  PubMed  CAS  Google Scholar 

  • Sundberg C, Al-Soud WA, Larsson M, Alm E, Yekta SS, Svensson BH, Sørensen SJ, Karlsson A (2013) 454 pyrosequencing analyses of bacterial and archaeal richness in 21 full-scale biogas digesters. FEMS Microbiol Ecol 85(3):612–626. doi:10.1111/1574-6941.12148

    Article  PubMed  CAS  Google Scholar 

  • Werner JJ, Knights D, Garcia ML, Scalfone NB, Smith S, Yarasheski K, Cummings TA, Beers AR, Knight R, Angenent LT (2011) Bacterial community structures are unique and resilient in full-scale bioenergy systems. Proc Nat Acad Sci USA 108(10):4158–4163. doi:10.1073/pnas.1015676108

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhou JZ, Liu WZ, Deng Y, Jiang YH, Xue K, He ZL, Van Nostrand JD, Wu LY, Yang YF, Wang AJ (2013) Stochastic assembly leads to alternative communities with distinct functions in a bioreactor microbial community. Mbio 4(2). doi:10.1128/mBio.00584-12

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Acknowledgments

This study was funded by the Yangfan project from Science and Technology Commission of Shanghai Municipality (14YF1400400), National Natural Science Foundation of China (51408133), and SRF for ROCS, SEM.

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Authors and Affiliations

  1. School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai, 201418, China

    Sheng Han & Yafeng Liu

  2. Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai, 200433, China

    Yafeng Liu, Shicheng Zhang & Gang Luo

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  1. Sheng Han
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Correspondence to Gang Luo.

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Han, S., Liu, Y., Zhang, S. et al. Reactor performances and microbial communities of biogas reactors: effects of inoculum sources. Appl Microbiol Biotechnol 100, 987–995 (2016). https://doi.org/10.1007/s00253-015-7062-7

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