Abstract
Biotechnological production of vanillin is gaining momentum as the natural synthesis of vanillin that is very expensive. Ferulic acid (FA), a costly compound, is used as the substrate to produce vanillin biotechnologically and the making process is still expensive. Therefore, this study investigated the practical use of an agrobiomass waste, rice bran, and provides the first evidence of a cost-effective production of vanillin within 24 h of incubation using recombinant Pediococcus acidilactici BD16 (fcs +/ech +). Introduction of two genes encoding feruloyl CoA synthetase and enoyl CoA hydratase into the native strain increased vanillin yield to 4.01 g L−1. Bioconversion was monitored through the transformation of phenolic compounds. A hypothetical metabolic pathway of rice bran during the vanillin bioconversion was proposed with the inserted pathway from ferulic acid to vanillin and compared with that of other metabolic engineered strains. These results could be a gateway of using recombinant lactic acid bacteria for industrial production of vanillin from agricultural waste.
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Acknowledgements
Authors acknowledge University Grants Commission, New Delhi, India for providing UGC major research project to Dr. Baljinder Kaur and meritorious scholarship to Mr. Debkumar Chakraborty and Masafumi Noda for providing cloning vector pLES003.
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The authors acknowledge the UGC major research project entitled “Metabolic engineering of a lactic acid bacterial isolate for biotransformation of ferulic acid to vanillin” to Dr. Baljinder Kaur (F.39-271/2010(SR)).
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Chakraborty, D., Selvam, A., Kaur, B. et al. Application of recombinant Pediococcus acidilactici BD16 (fcs +/ech +) for bioconversion of agrowaste to vanillin. Appl Microbiol Biotechnol 101, 5615–5626 (2017). https://doi.org/10.1007/s00253-017-8283-8
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DOI: https://doi.org/10.1007/s00253-017-8283-8