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Establishment of a loop-mediated isothermal amplification (LAMP) detection method for genetically modified maize MON88017

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Abstract

In this study, we developed a visual and rapid assay for the detection of MON88017 maize using the LAMP method. The LAMP method was specific for MON88017 event and takes only 40 min and the LAMP assay sensitivity is about 40 copies, which is the same level as that of conventional PCR method. LAMP amplicons can directly be detected by naked-eye inspection after adding SYBR Green I. In summary, the LAMP method is visual, faster, and more sensitive and does not need special equipment compared to the traditional PCR technique, which makes it a very higher efficiency approach for field tests and fast screening of GMO crops, especially for on-site, large-scale testing purposes in the field.

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Acknowledgments

This study was supported by Natural Science Foundation of Heilongjiang Province of China (No. C2015003), National Natural Science Foundation of China (No. J1210069), Doctor Scientific Research Initial Foundation of Northeast Agricultural University (No. 2012RCB19) and the Discipline Team Construction Project of Northeast Agricultural University.

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Authors and Affiliations

  1. Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, Heilongjiang, China

    Zhen Zhen, Minghui Zhang, Yanbo Yu, Xuejun Gao, Yanming Zhu, Yu Yan & Renren Zhang

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  1. Zhen Zhen
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  2. Minghui Zhang
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  3. Yanbo Yu
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  4. Xuejun Gao
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  5. Yanming Zhu
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  6. Yu Yan
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Correspondence to Minghui Zhang.

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Zhen, Z., Zhang, M., Yu, Y. et al. Establishment of a loop-mediated isothermal amplification (LAMP) detection method for genetically modified maize MON88017. Eur Food Res Technol 242, 1787–1793 (2016). https://doi.org/10.1007/s00217-016-2678-0

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  • DOI: https://doi.org/10.1007/s00217-016-2678-0

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