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Loop-Mediated Isothermal Amplification for the Event-Specific Detection of Wheat B73-6-1

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Abstract

To develop effective alternatives for detecting genetically modified organisms, we constructed one loop-mediated isothermal amplification (LAMP) event-specific detection method for wheat B73-6-1 in this study. This event-specific LAMP assay can be performed within 38 min without any PCR equipment and the LAMP products can be directly observed by the naked eye instead of conventional gel electrophoresis analysis. The limits of detection of these established visual LAMP assays were about six copies of haploid wheat-genomic DNA. Furthermore, the high specificity of LAMP assays was determined. High specificity and sensitivity, cost-effectiveness, and low requirement of equipment of the developed event-specific LAMP assay of B73-6-1 allow this method to be useful in transgenic wheat samples analysis, especially in highly processed products.

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Acknowledgments

This work was supported by General Administration of Quality Supervision, Inspection and Quarantine of P. R. C. (2011B286k), the National Natural Science Foundation of China (J1210069/J0106) and Heilongjiang Bureau Program of China (2013HK001).

Conflict of Interest

Yang Cheng declares that she has no conflict of interest. Minghui Zhang declares that she has no conflict of interest. Kun Hu declares that she has no conflict of interest. Fangda Sun declares that he has no conflict of interest. Ran Tao declares that she has no conflict of interest. Xuejun Gao declares that he has no conflict of interest. Fengxia Luan declares that she has no conflict of interest. This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Heilongjiang Entry–Exit Inspection and Quarantine Bureau, Harbin, People’s Republic of China

    Yang Cheng, Kun Hu & Fengxia Luan

  2. Detecting Center of Agricultural Genetically Modified Organism, Research Center of Life Science and Biotechnique, Northeast Agricultural University, Harbin, People’s Republic of China

    Minghui Zhang, Fangda Sun & Xuejun Gao

  3. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, People’s Republic of China

    Ran Tao

Authors
  1. Yang Cheng
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  2. Minghui Zhang
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  3. Kun Hu
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  4. Fangda Sun
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  5. Ran Tao
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  6. Xuejun Gao
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  7. Fengxia Luan
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Corresponding author

Correspondence to Fengxia Luan.

Additional information

Yang Cheng and Minghui Zhang contributed equally to this work.

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Cheng, Y., Zhang, M., Hu, K. et al. Loop-Mediated Isothermal Amplification for the Event-Specific Detection of Wheat B73-6-1. Food Anal. Methods 7, 500–505 (2014). https://doi.org/10.1007/s12161-013-9718-1

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  • DOI: https://doi.org/10.1007/s12161-013-9718-1

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