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A sensitive biosensor for determination of pathogenic bacteria using aldehyde dehydrogenase signaling system

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Abstract

Aldehyde dehydrogenase (ALDH) was first developed as an enzymatic signaling system of a biosensor for sensitive point-of-care detection of pathogenic bacteria. ALDH and specific aptamers to Salmonella typhimurium (S. typhimurium), as organic components, were embedded in organic-inorganic nanocomposites as a biosensor signal label, integrating the functions of signal amplification and target recognition. The biosensing mechanism is based on the fact that ALDH can catalyze rapid oxidation of acetaldehyde into acetic acid, resulting in pH change with portable pH meter readout. The altered pH exhibited a linear relationship with the logarithm of S. typhimurium from 102 to 108 CFU/mL and detection limit of 46 CFU/mL. Thus, the proposed biosensor has potential application in the diagnosis of pathogenic bacteria.

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Funding

This work was financially supported by the National Key Research and Development Program of China (No. 2016YFD0501001).

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Authors and Affiliations

  1. College of Life Science, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Wenguang Zhang & Zehong Li

  2. Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, 130122, Jilin, China

    Wenguang Zhang, Shengjun Bu, Huasong Bai, Li Ma, Hongguo Wei, Xiu Liu & Jiayu Wan

  3. College of Geo-Exploration Science and Technology, Jilin University, Changchun, 130026, Jilin, China

    Chengyou Ma

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  1. Wenguang Zhang
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  5. Li Ma
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Zhang, W., Bu, S., Bai, H. et al. A sensitive biosensor for determination of pathogenic bacteria using aldehyde dehydrogenase signaling system. Anal Bioanal Chem 412, 7955–7962 (2020). https://doi.org/10.1007/s00216-020-02928-7

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